Optical control of dopamine receptors using a photoswitchable tethered inverse agonist

Donthamsetti et al., 2017, JACS

Restoration of patterned vision with an engineered photoactivatable G-protein coupled receptor

Berry et al., 2017, Nat. Comm.

Precise modulation of neuronal activity with synthetic photoswitchable ligands

Kienzler & Isacoff, 2017, Curr. Opin. Neurobiol.

Sequential steps of CRAC channel activation

Palty, Fu & Isacoff, 2017, Cell Rep.

Synapses in the spotlight with synthetic optogenetics

Berlin & Isacoff, 2017, EMBO Rep.

A new mechanism of voltage-dependent gating exposed by Kv10.1 channels interrupted between voltage sensor and pore

Tomczak et al., 2017, J. Gen. Physiol.

Dual optical control and mechanistic insights into photoswitchable group II and III metabotropic glutamate receptors

Levitz et al., 2017, PNAS

Measuring behavioral individuality in the acoustic startle behavior in zebrafish

Pantoja et al., 2017, Bio Protoc.

Input-specific plasticity and homeostasis at the Drosophila larval neuromuscular junction

Newman et al., 2017, Neuron

Mechanism of assembly and cooperativity of homomeric and heteromeric metabotropic glutamate receptors

Levitz et al., 2016, Neuron

Neuromodulatory regulation of behavioral individuality in zebrafish

Pantoja et al., 2016, Neuron

A family of photoswitchable NMDA receptors

Berlin et al., 2016, Elife

Caenorhabditis elegans paraoxonase-like proteins control the functional expression of DEG/ENaC mechanosensory proteins

Chen et al., 2016, Mol. Biol. Cell

Heterodimerization within the TREK channel subfamily produces a diverse family of highly regulated potassium channels

Levitz et al., 2016, PNAS

Allosteric substrate switching in a voltage-sensing lipid phosphatase

Grimm & Isacoff, 2016, Nat. Chem. Biol.

A toolkit for orthogonal and in vivo optical manipulation of ionotropic glutamate receptors

Levitz et al., 2016, Front. Mol. Neurosci.

Cooperative binding of stromal interaction molecule 1 (STIM1) to the N and C termini of calcium release-activated calcium modulator 1 (Orai1)

Palty & Isacoff, 2015, J. Biol. Chem.

Optical control of neuronal activity using a light-operated GIRK channel opener (LOGO)

Barber et al., 2015, Chem. Sci.

Orthogonal optical control of a G protein-coupled receptor with a SNAP-tethered photochromic ligand

Broichhagen et al., 2015, ACS Cent. Sci.

Optogenetic vision restoration using rhodopsin for enhanced sensitivity

Gaub et al., 2015, Mol. Ther.

Conformational dynamics of a class C G-protein-coupled receptor

Vafabakhsh, Levitz & Isacoff, 2015, Nature

Subunit composition of a DEG/ENaC mechanosensory channel of Caenorhabditis elegans

Chen et al., 2015, PNAS

Photoactivatable genetically encoded calcium indicators for targeted neuronal imaging

Berlin et al., 2015, Nat. Methods

Critical role for Orai C-terminal domain and TM4 in CRAC channel gating

Palty, Stanley & Isacoff, 2015, Cell Res.

A specialized molecular motion opens the Hv1 voltage-gated proton channel

Mony, Berger & Isacoff, 2015, Nat. Struct. Mol. Biol.

Two-photon brightness of azobenzene photoswitches designed for glutamate receptor optogenetics

Carroll et al., 2015, PNAS

Controlling ionotropic and metabotropic glutamate receptors with light: principles and potential

Reiner, Levitz & Isacoff, 2015, Curr. Opin. Pharmacol.

A spinal opsin controls early neural activity and drives a behavioral light response

Friedmann et al., 2015, Curr. Biol.

Restoration of visual function by expression of a light-gated mammalian ion channel in retinal ganglion cells or ON-bipolar cells

Gaub et al., 2014, PNAS

Fluorescent labeling for patch-clamp fluorometry (PCF) measurements of real-time protein motion in ion channels

Berger & Isacoff, 2014, Methods Mol. Biol.

Phospholipase D2 specifically regulates TREK potassium channels via direct interaction and local production of phosphatidic acid

Comoglio et al., 2014, PNAS

Stoichiometry and specific assembly of Best ion channels

Bharill et al., 2014, PNAS

Photoswitching of cell surface receptors using tethered ligands

Reiner & Isacoff, 2014, Methods Mol. Biol.

Evoked and spontaneous transmission favored by distinct sets of synapses

Peled, Newman & Isacoff, 2014, Curr. Biol.

Tethered ligands reveal glutamate receptor desensitization depends on subunit occupancy

Reiner & Isacoff, 2014, Nat. Chem. Biol.

A red-shifted, fast-relaxing azobenzene photoswitch for visible light control of an ionotropic glutamate receptor

Kienzler et al., 2013, JACS

Measuring membrane voltage with fluorescent proteins

Patti & Isacoff, 2013, CSH Protocols

Rapid feedback regulation of synaptic efficacy during high-frequency activity at the Drosophila larval neuromuscular junction

Kauwe & Isacoff, 2013, PNAS

AMPA receptor/TARP stoichiometry visualized by single-molecule subunit counting

Hastie et al., 2013, PNAS

Optical control of metabotropic glutamate receptors

Levitz et al., 2013, Nat. Neurosci.

Molecular mechanisms of the assembly of an acid-sensing receptor ion channel complex

Yu et al., 2012, Nat. Comms.

Optical control of endogenous proteins with a photoswitchable conditional subunit reveals a role for TREK1 in GABA(B) signaling

Sandoz et al., 2012, Neuron

A glutamate switch controls voltage-sensitive phosphatase function

Liu et al., 2012, Nat. Struct. Mol. Biol.

Assembly stoichiometry of the GluK2/GluK5 kainate receptor complex

Reiner, Arant & Isacoff, 2012, Cell Rep.

Emergence of patterned activity in the developing zebrafish spinal cord

Warp et al., 2012, Curr. Biol.

The pore of the voltage-gated proton channel

Berger & Isacoff, 2011, Neuron

Optical quantal analysis of synaptic transmission in wild-type and rab3 mutant Drosophila motor axons

Caporale et al., 2011, Mol. Ther.

Specializations of a pheromonal glomerulus in the Drosophila olfactory system

Agarwal & Isacoff, 2011, J. Neurophys.

A modern ionotropic glutamate receptor with a K(+) selectivity signature sequence

Janovjak, Sandoz & Isacoff, 2011, Nat. Comm.

Optical quantal analysis of synaptic transmission in wild-type and rab3 mutant Drosophila motor axons

Peled & Isacoff, 2011, Nat. Neurosci.

Optical probing of a dynamic membrane interaction that regulates the TREK1 channel

Sandoz, Bell & Isacoff, 2011, PNAS

Stoichiometry of the KCNQ1-KCNE1 ion channel complex

Nakajo et al., 2010, PNAS

Filtering of visual information in the tectum by an identified neural circuit

Del Bene et al., 2010, Science

Multiple C-terminal tail Ca2+/CaMs regulate Cav1.2 function but do not mediate channel dimerization

Kim et al., 2010, EMBOJ

Alternative splicing of neuroligin regulates the rate of presynaptic differentiation

Hanson, Dean & Isacoff, 2010, J. Neurosci.

A light-gated, potassium-selective glutamate receptor for the optical inhibition of neuronal activity

Janovjak et al., 2010, Nat. Neurosci.

Electrochemical coupling in the voltage-dependent phosphatase Ci-VSP

Kohout et al., 2010, Nat. Chem. Biol.

Green fluorescent proteins (GFPs) for measuring voltage

Siegel & Isacoff, 2010, CSH Protocols

Optical control of neuronal activity

Szobota & Isacoff, 2010, Annu. Rev. Biophys.

The opening of the two pores of Hv1 voltage-gated proton channel is tuned by cooperativity

Tombola et al., 2009, Nat. Struct. Mol. Biol.

Optogenetic dissection of a behavioural module in the vertebrate spinal cord

Wyart et al., 2009, Nature

Nanosculpting reversed wavelength sensitivity into a photoswitchable iGluR

Numano et al., 2009, PNAS

Rules of engagement for NMDA receptor subunits

Ulbrich & Isacoff, 2008, PNAS

Colloid-guided assembly of oriented 3D neuronal networks

Pautot, Wyart & Isacoff, 2008, Nat. Methods

The voltage-gated proton channel Hv1 has two pores, each controlled by one voltage sensor

Tombola, Ulbrich & Isacoff, 2008, Neuron

Subunit organization and functional transitions in Ci-VSP

Kohout et al., 2007, Nat. Struct. Mol. Biol.

All optical interface for parallel, remote, and spatiotemporal control of neuronal activity

Wang et al., 2007, Nano Letters

Drosophila huntingtin-interacting protein 14 is a presynaptic protein required for photoreceptor synaptic transmission and expression of the palmitoylated proteins synaptosome-associated protein 25 and cysteine string protein

Stowers & Isacoff, 2007, J. Neurosci.

Closing in on the resting state of the Shaker K(+) channel

Pathak et al., 2007, Neuron

Mechanisms of photoswitch conjugation and light activation of an ionotropic glutamate receptor

Gorostiza et al., 2007, PNAS

Remote control of neuronal activity with a light-gated glutamate receptor

Szobota et al., 2007, Neuron

Molecular imaging of hydrogen peroxide produced for cell signaling

Miller et al., 2007, Nat. Chem. Biol.

Subunit counting in membrane-bound proteins

Ulbrich & Isacoff, 2007, Nat. Methods

Reversibly caged glutamate: a photochromic agonist of ionotropic glutamate receptors

Volgraf et al., 2007, JACS

The twisted ion-permeation pathway of a resting voltage-sensing domain

Tombola et al., 2007, Nature

A selective turn-on fluorescent sensor for imaging copper in living cells

Zeng et al., 2006, JACS

Allosteric control of an ionotropic glutamate receptor with an optical switch

Volgraf et al., 2006, Nat. Chem. Biol.

Molecular handles for the mechanical manipulation of single-membrane proteins in living cells

Gorostiza et al., 2005, IEEE Trans. Nanobioscience

Boronate-based fluorescent probes for imaging cellular hydrogen peroxide

Miller et al., 2005, JACS

Heterogeneity in synaptic transmission along a Drosophila larval motor axon

Guerrero et al., 2005, Nat Neurosci

In vivo performance of genetically encoded indicators of neural activity in flies

Reiff et al., 2005, J. Neurosci.

Voltage-sensing arginines in a potassium channel permeate and occlude cation-selective pores

Tombola, Pathak & Isacoff, 2005, Neuron

A fluorescent probe designed for studying protein conformational change

Cohen et al., 2005, PNAS

The cooperative voltage sensor motion that gates a potassium channel

Pathak et al., 2005, J. Gen. Physiol.

A selective, cell-permeable optical probe for hydrogen peroxide in living cells

Chang et al., 2004, JACS

Light-activated ion channels for remote control of neuronal firing

Banghart et al., 2004, Nat. Neurosci.

The orientation and molecular movement of a K(+) channel voltage-sensing domain

Gandhi et al., 2003, Neuron

Protein surface recognition by rational design: nanomolar ligands for potassium channels

Gradl et al., 2003, JACS

Neurexin mediates the assembly of presynaptic terminals

Dean et al., 2003, Nat. Neurosci.

Tuning FlaSh: Redesign of the Dynamics, Voltage Range, and Color of the Genetically Encoded Optical Sensor of Membrane Potential

Guerrero et al., 2002, Biophysical Journal

Structural rearrangements in single ion channels detected optically in living cells

Sonnleitner et al., 2002, PNAS

Conformational switch between slow and fast gating modes: allosteric regulation of voltage sensor mobility in the EAG K+ channel

Schonherr et al., 2002, Neuron

Molecular coupling of S4 to a K(+) channel's slow inactivation gate

Loots & Isacoff, 2000, J. Gen. Physiol.

Reconstructing voltage sensor-pore interaction from a fluorescence scan of a voltage-gated K+ channel

Gandhi, Loots & Isacoff, 2000, Neuron

Green fluorescent protein-based sensors for detecting signal transduction and monitoring ion channel function

Siegel & Isacoff, 2000, Methods Enzymol.

Independence and cooperativity in rearrangements of a potassium channel voltage sensor by single subunit fluorescence

Mannuzzu & Isacoff, 2000, J. Gen. Physiol.

Spectroscopic mapping of voltage sensor movement in the Shaker potassium channel

Glauner et al., 1999, Nature

Watching a synapse grow: noninvasive confocal imaging of synaptic growth in Drosophila

Zito et al., 1999, Neuron

Protein rearrangements underlying slow inactivation of the Shaker K+ channel

Loots & Isacoff, 1998, J. Gen. Physiol.

Three transmembrane conformations and sequence-dependent displacement of the S4 domain in shaker K+ channel gating

Baker et al., 1998, Neuron

Synaptic clustering of Fasciclin II and Shaker: essential targeting sequences and role of Dlg

Zito et al., 1997, Neuron

A genetically encoded optical probe of membrane voltage

Siegel & Isacoff, 1997, Neuron

Hydrophobic mutations alter the movement of Mg2+ in the pore of voltage-gated potassium channels

Harris & Isacoff, 1996, Biophysical Journal

Transmembrane movement of the Shaker K+ channel S4

Larsson et al., 1996, Neuron

Direct physical measure of conformational rearrangement underlying potassium channel gating

Mannuzzu, Moronne & Isacoff, 1996, Science

Putative receptor for the cytoplasmic inactivation gate in the Shaker K+ channel

Isacoff, Jan & Jan, 1991, Nature

Evidence for the formation of heteromultimeric potassium channels in Xenopus oocytes

Isacoff, Jan & Jan, 1990, Nature

Burst-patterned stimulation promotes nicotinic transmission in isolated perfused rat sympathetic ganglia

Birks & Isacoff, 1988, The Journal of Physiology