Cell Biology and Biochemistry

2013-present

Good MC, Vahey MD, Skandarajah A, Fletcher DA, Heald R. Cytoplasmic volume modulates spindle size during embryogenesis. Science 2013 Nov 15;342(6160):856-60. PubMed PMID:24233724. PDF

Schlaitz AL, Thompson J, Wong CC, Yates JR 3rd, Heald R. REEP3/4 ensure endoplasmic reticulum clearance from metaphase chromatin and proper nuclear envelope architecture. Dev. Cell 2013 Aug 12;26(3):315-23. PubMed PMID: 23911198l; PubMed Central PMCID: PMC3745822.PDF

Wilbur JD and Heald R. Cryptic No longer: Arrays of CLASP1 TOG Domains. Structure 2013 Jun 4;21(6):869-70. PubMed PMID: 23747108. PDF

Bird SL, Heald R, Weis K. RanGTP and CLASP1 cooperate to position the mitotic spindle. Mol. Biol. Cell  Aug;24(16):2506-14. PubMed PMID: 23783028; PubMed Central PMCID:PMC3744954. PDF

Helmke KJ, Heald R, Wilbur JD. Interplay between spindle architecture and function. Int'l Rev.of Cell and Mol. Biol. 2013. 306:83-125. PubMed PMID: 24016524.PDF

Wilbur JD, Heald R. Mitotic spindle scaling during Xenopus development by kif2a and importin α. Elife. 2013; 2:e00290. doi: 10.7554/eLife.00290. Epub 2013 Feb 19. PubMed PMID: 23425906; PubMed Central PMCID: PMC3576809.PDF

Whitehead E, Heald R, Wilbur JD. N-terminal phosphorylation of p60 katanin directly regulated microtubule severing. J Mol. Biol. 2013 Jan 23; 425(2):214-21. PubMed PMID:23178168; PubMed Central PMCID: PMC3540178. PDF

2012

Xiao B, Freedman BS, Miller KE, Jeald R, Marko JF. Histone H1 compact DNA under force and during chromatin assembly. Mol. Biol. Cell. 2012 Dec;23(24):4864-71. PubMed PMID: 23097493; PubMed Central PMCID: PMC3521694.PDF

Riggs B, Berhman ZJ, Heald R. Altering membrane topology with Sar1 does not impair spindle assembly in Xenopus egg extracts. Cytoskeleton (Hoboken). 2012 Aug;69(8):591-9. PubMed PMID: 22605651. PDF

Patel K, Nogales E, Heald R. Multiple domains of human CLASP contribute to
microtubule dynamics and organization in vitro and in Xenopus egg extracts.
Cytoskeleton (Hoboken). 2012 Mar; 69(3):155-65. PubMed PMID: 22278908; PubMed Central PMCID: PMC3315288. PDF

2011

Loughlin R, Wilbur JD, McNally FJ, Nédélec FJ, Heald R. Katanin contributes to
interspecies spindle length scaling in Xenopus. Cell. 2011 Dec 9;147(6):1397-407.
PubMed PMID: 22153081; PubMed Central PMCID: PMC3240848. PDF

Halpin D, Kalab P, Wang J, Weis K, Heald R. Mitotic spindle assembly around
RCC1-coated beads in Xenopus egg extracts. PLoS Biol. 2011 Dec;9(12):e1001225.
Epub 2011 Dec 27. PubMed PMID: 22215983; PubMed Central PMCID: PMC3246454. PDF

Kieserman EK, Heald R. Mitotic chromosome size scaling in Xenopus. Cell Cycle.
2011 Nov 15;10(22):3863-70. Epub 2011 Nov 15. PubMed PMID: 22071695; PubMed
Central PMCID: PMC3266116. PDF

Soderholm JF, Bird SL, Kalab P, Sampathkumar Y, Hasegawa K, Uehara-Bingen M,
Weis K, Heald R. Importazole, a small molecule inhibitor of the transport
receptor importin-β. ACS Chem Biol. 2011 Jul 15;6(7):700-8. Epub 2011 Apr 21.
PubMed PMID: 21469738; PubMed Central PMCID: PMC3137676. PDF

Fu H, Freedman BS, Lim CT, Heald R, Yan J. Atomic force microscope imaging of
chromatin assembled in Xenopus laevis egg extract. Chromosoma. 2011
Jun;120(3):245-54. doi: 10.1007/s00412-010-0307-4. Epub 2011 Jan 11. PubMed PMID:
21369955. PDF

Ridley A, Heald R. Cell structure and dynamics. Curr Opin Cell Biol. 2011
Feb;23(1):1-3. Epub 2010 Dec 27. PubMed PMID: 21190823.

2010

Loughlin R, Heald R, Nédélec F. A computational model predicts Xenopus meiotic
spindle organization. J Cell Biol. 2010 Dec 27;191(7):1239-49. Epub 2010 Dec 20.
PubMed PMID: 21173114; PubMed Central PMCID: PMC3010074. PDF

Levy DL, Heald R. Nuclear size is regulated by importin α and Ntf2 in Xenopus.
Cell. 2010 Oct 15;143(2):288-98. PubMed PMID: 20946986; PubMed Central PMCID:
PMC2966892. PDF

Freedman BS, Miller KE, Heald R. Xenopus egg extracts increase dynamics of
histone H1 on sperm chromatin. PLoS One. 2010 Sep 29;5(9). pii: e13111. PubMed
PMID: 20927327; PubMed Central PMCID: PMC2947519. PDF

Heald R. Rebecca Heald. Curr Biol. 2010 Jun 22;20(12):R503-4. PubMed PMID:
20627848.

Freedman BS, Heald R. Functional comparison of H1 histones in Xenopus reveals
isoform-specific regulation by Cdk1 and RanGTP. Curr Biol. 2010 Jun
8;20(11):1048-52. Epub 2010 May 13. PubMed PMID: 20471264; PubMed Central PMCID:
PMC2902237. PDF

2009

Srsen V, Fant X, Heald R, Rabouille C, Merdes A. Centrosome proteins form an
insoluble perinuclear matrix during muscle cell differentiation. BMC Cell Biol.
2009 Apr 21;10:28. PubMed PMID: 19383121; PubMed Central PMCID: PMC2676252.

2008

Heald R. Rebecca Heald: passionate about cycles. Interview by Caitlin
Sedwick. J Cell Biol. 2008 Nov 17;183(4):570-1. PubMed PMID: 19015313; PubMed
Central PMCID: PMC2582880.

Loughlin R, Riggs B, Heald R. SnapShot: motor proteins in spindle assembly.
Cell. 2008 Aug 8;134(3):548-548.e1. PubMed PMID: 18692476.

Kalab P, Heald R. The RanGTP gradient - a GPS for the mitotic spindle. J Cell
Sci. 2008 May 15;121(Pt 10):1577-86. PubMed PMID: 18469014.

Andersen CB, Wan Y, Chang JW, Riggs B, Lee C, Liu Y, Sessa F, Villa F,
Kwiatkowski N, Suzuki M, Nallan L, Heald R, Musacchio A, Gray NS. Discovery of
selective aminothiazole aurora kinase inhibitors. ACS Chem Biol. 2008 Mar
20;3(3):180-92. Epub 2008 Feb 29. PubMed PMID: 18307303.

Walczak CE, Heald R. Mechanisms of mitotic spindle assembly and function. Int
Rev Cytol. 2008;265:111-58. Review. PubMed PMID: 18275887.

2007

Blower MD, Feric E, Weis K, Heald R. Genome-wide analysis demonstrates
conserved localization of messenger RNAs to mitotic microtubules. J Cell Biol.
2007 Dec 31;179(7):1365-73. PubMed PMID: 18166649; PubMed Central PMCID:
PMC2373496.

Heald R. Brinkley-fest of mitosis. Dev Cell. 2007 Aug;13(2):168-76. PubMed
PMID: 17681129.

Brown KS, Blower MD, Maresca TJ, Grammer TC, Harland RM, Heald R. Xenopus
tropicalis egg extracts provide insight into scaling of the mitotic spindle. J
Cell Biol. 2007 Mar 12;176(6):765-70. Epub 2007 Mar 5. PubMed PMID: 17339377;
PubMed Central PMCID: PMC2064050.

Yan J, Maresca TJ, Skoko D, Adams CD, Xiao B, Christensen MO, Heald R, Marko
JF. Micromanipulation studies of chromatin fibers in Xenopus egg extracts reveal
ATP-dependent chromatin assembly dynamics. Mol Biol Cell. 2007 Feb;18(2):464-74.
Epub 2006 Nov 15. PubMed PMID: 17108322; PubMed Central PMCID: PMC1783770.

2006

Kenney RD, Heald R. Essential roles for cohesin in kinetochore and spindle
function in Xenopus egg extracts. J Cell Sci. 2006 Dec 15;119(Pt 24):5057-66.
PubMed PMID: 17158911.

Kaláb P, Pralle A, Isacoff EY, Heald R, Weis K. Analysis of a
RanGTP-regulated gradient in mitotic somatic cells. Nature. 2006 Mar
30;440(7084):697-701. PubMed PMID: 16572176.

Maresca TJ, Heald R. The long and the short of it: linker histone H1 is
required for metaphase chromosome compaction. Cell Cycle. 2006 Mar;5(6):589-91.
Epub 2006 Mar 15. Review. PubMed PMID: 16582611.

Soderholm J, Uehara-Bingen M, Weis K, Heald R. Challenges facing the
biologist doing chemical genetics. Nat Chem Biol. 2006 Feb;2(2):55-8. PubMed
PMID: 16421579.

Heald R. Cell biology. Serving up a plate of chromosomes. Science. 2006 Jan
20;311(5759):343-4. PubMed PMID: 16424328.

Hannak E, Heald R. Xorbit/CLASP links dynamic microtubules to chromosomes in
the Xenopus meiotic spindle. J Cell Biol. 2006 Jan 2;172(1):19-25. PubMed PMID:
16390996; PubMed Central PMCID: PMC2063525.

Budde PP, Desai A, Heald R. Analysis of microtubule polymerization in vitro
and during the cell cycle in Xenopus egg extracts. Methods. 2006 Jan;38(1):29-34.
PubMed PMID: 16343934.

Maresca TJ, Heald R. Methods for studying spindle assembly and chromosome
condensation in Xenopus egg extracts. Methods Mol Biol. 2006;322:459-74. Review.
PubMed PMID: 16739744.

Hannak E, Heald R. Investigating mitotic spindle assembly and function in
vitro using Xenopus laevis egg extracts. Nat Protoc. 2006;1(5):2305-14. PubMed
PMID: 17406472.

2005

Maresca TJ, Niederstrasser H, Weis K, Heald R. Xnf7 contributes to spindle
integrity through its microtubule-bundling activity. Curr Biol. 2005 Oct
11;15(19):1755-61. PubMed PMID: 16213823.

Maresca TJ, Heald R. Chromosome congression: another fine mesh we've gotten
into. Dev Cell. 2005 Sep;9(3):314-5. Review. PubMed PMID: 16139222.

Maresca TJ, Freedman BS, Heald R. Histone H1 is essential for mitotic
chromosome architecture and segregation in Xenopus laevis egg extracts. J Cell
Biol. 2005 Jun 20;169(6):859-69. PubMed PMID: 15967810; PubMed Central PMCID:
PMC2171634.

Blower MD, Nachury M, Heald R, Weis K. A Rae1-containing ribonucleoprotein
complex is required for mitotic spindle assembly. Cell. 2005 Apr
22;121(2):223-34. PubMed PMID: 15851029.

Soderholm J, Heald R. Scratch n' screen for inhibitors of cell migration.
Chem Biol. 2005 Mar;12(3):263-5. Review. PubMed PMID: 15797209.

2004

Banks JD, Heald R. Adenomatous polyposis coli associates with the
microtubule-destabilizing protein XMCAK. Curr Biol. 2004 Nov 23;14(22):2033-8.
PubMed PMID: 15556866.

Gadde S, Heald R. Mechanisms and molecules of the mitotic spindle. Curr Biol.
2004 Sep 21;14(18):R797-805. Review. PubMed PMID: 15380094.

Deehan R, Heald R. Centromere glue provides spindle cue. Cell. 2004 Sep
3;118(5):529-30. Review. PubMed PMID: 15339655.

Skop AR, Liu H, Yates J 3rd, Meyer BJ, Heald R. Dissection of the mammalian
midbody proteome reveals conserved cytokinesis mechanisms. Science. 2004 Jul
2;305(5680):61-6. Epub 2004 May 27. PubMed PMID: 15166316.

Krauss SW, Lee G, Chasis JA, Mohandas N, Heald R. Two protein 4.1 domains
essential for mitotic spindle and aster microtubule dynamics and organization in
vitro. J Biol Chem. 2004 Jun 25;279(26):27591-8. Epub 2004 Apr 21. PubMed PMID:
15102852.

Heald RW. Cell division: burning the spindle at both ends. Nature. 2004 Jan
22;427(6972):300-1. PubMed PMID: 14737150.

Wignall SM, Gray NS, Chang YT, Juarez L, Jacob R, Burlingame A, Schultz PG,
Heald R. Identification of a novel protein regulating microtubule stability
through a chemical approach. Chem Biol. 2004 Jan;11(1):135-46. PubMed PMID:
15113003.

2003

Wignall SM, Heald R. The role of chromosome architecture in spindle assembly
and anaphase: the condensed version. Cell Cycle. 2003 Nov-Dec;2(6):590-1. PubMed
PMID: 14512775.

Krauss SW, Chen C, Penman S, Heald R. Nuclear actin and protein 4.1:
essential interactions during nuclear assembly in vitro. Proc Natl Acad Sci U S
A. 2003 Sep 16;100(19):10752-7. Epub 2003 Sep 5. PubMed PMID: 12960380; PubMed
Central PMCID: PMC196875.

Wignall SM, Deehan R, Maresca TJ, Heald R. The condensin complex is required
for proper spindle assembly and chromosome segregation in Xenopus egg extracts. J
Cell Biol. 2003 Jun 23;161(6):1041-51. PubMed PMID: 12821643; PubMed Central
PMCID: PMC2172990.

Budde PP, Heald R. Centrosomes and kinetochores, who needs 'Em? The role of
noncentromeric chromatin in spindle assembly. Curr Top Dev Biol. 2003;56:85-113.
Review. PubMed PMID: 14584727.

2002

Krauss SW, Heald R, Lee G, Nunomura W, Gimm JA, Mohandas N, Chasis JA. Two
distinct domains of protein 4.1 critical for assembly of functional nuclei in
vitro. J Biol Chem. 2002 Nov 15;277(46):44339-46. Epub 2002 Aug 8. PubMed PMID:
12171917.

Kalab P, Weis K, Heald R. Visualization of a Ran-GTP gradient in interphase
and mitotic Xenopus egg extracts. Science. 2002 Mar 29;295(5564):2452-6. PubMed
PMID: 11923538.

Heald R, Nogales E. Microtubule dynamics. J Cell Sci. 2002 Jan 1;115(Pt
1):3-4. PubMed PMID: 11801717.

2001

Chang YT, Wignall SM, Rosania GR, Gray NS, Hanson SR, Su AI, Merlie J Jr,
Moon HS, Sangankar SB, Perez O, Heald R, Schultz PG. Synthesis and biological
evaluation of myoseverin derivatives: microtubule assembly inhibitors. J Med
Chem. 2001 Dec 20;44(26):4497-500. PubMed PMID: 11741468.

Van Hooser AA, Heald R. Kinetochore function: the complications of becoming
attached. Curr Biol. 2001 Oct 30;11(21):R855-7. Review. PubMed PMID: 11696340.

Merlie J Jr, Heald R. Mitotic spindle assembly in vitro. Curr Protoc Cell
Biol. 2001 May;Chapter 11:Unit 11.13. PubMed PMID: 18228304.

Budde PP, Kumagai A, Dunphy WG, Heald R. Regulation of Op18 during spindle
assembly in Xenopus egg extracts. J Cell Biol. 2001 Apr 2;153(1):149-58. PubMed
PMID: 11285281; PubMed Central PMCID: PMC2185528.

Banks JD, Heald R. Chromosome movement: dynein-out at the kinetochore. Curr
Biol. 2001 Feb 20;11(4):R128-31. Review. PubMed PMID: 11250166.

Nachury MV, Maresca TJ, Salmon WC, Waterman-Storer CM, Heald R, Weis K.
Importin beta is a mitotic target of the small GTPase Ran in spindle assembly.
Cell. 2001 Jan 12;104(1):95-106. PubMed PMID: 11163243.

Wignall SM, Heald R. Methods for the study of centrosome-independent spindle
assembly in Xenopus extracts. Methods Cell Biol. 2001;67:241-56. PubMed PMID:
11550472.

2000

Heald R. Motor function in the mitotic spindle. Cell. 2000 Aug
18;102(4):399-402. Review. PubMed PMID: 10966101.

Merdes A, Heald R, Samejima K, Earnshaw WC, Cleveland DW. Formation of
spindle poles by dynein/dynactin-dependent transport of NuMA. J Cell Biol. 2000
May 15;149(4):851-62. PubMed PMID: 10811826; PubMed Central PMCID: PMC2174573.

Heald R. A dynamic duo of microtubule modulators. Nat Cell Biol. 2000
Jan;2(1):E11-2. PubMed PMID: 10620815.

Heald R, Weis K. Spindles get the ran around. Trends Cell Biol. 2000
Jan;10(1):1-4. PubMed PMID: 10603469.

1998-1999

Rosania GR, Merlie J Jr, Gray N, Chang YT, Schultz PG, Heald R. A
cyclin-dependent kinase inhibitor inducing cancer cell differentiation:
biochemical identification using Xenopus egg extracts. Proc Natl Acad Sci U S A.
1999 Apr 27;96(9):4797-802. PubMed PMID: 10220373; PubMed Central PMCID:
PMC21771.

Heald R, Walczak CE. Microtubule-based motor function in mitosis. Curr Opin
Struct Biol. 1999 Apr;9(2):268-74. Review. PubMed PMID: 10322211.

Walczak CE, Vernos I, Mitchison TJ, Karsenti E, Heald R. A model for the
proposed roles of different microtubule-based motor proteins in establishing
spindle bipolarity. Curr Biol. 1998 Jul 30-Aug 13;8(16):903-13. PubMed PMID:
9707401.

Pre-1998

Heald R, Tournebize R, Habermann A, Karsenti E, Hyman A. Spindle assembly in
Xenopus egg extracts: respective roles of centrosomes and microtubule
self-organization. J Cell Biol. 1997 Aug 11;138(3):615-28. PubMed PMID: 9245790;
PubMed Central PMCID: PMC2141625.

Tournebize R, Heald R, Hyman A. Role of chromosomes in assembly of meiotic
and mitotic spindles. Prog Cell Cycle Res. 1997;3:271-84. Review. PubMed PMID:
9552422.

Heald R, Tournebize R, Blank T, Sandaltzopoulos R, Becker P, Hyman A,
Karsenti E. Self-organization of microtubules into bipolar spindles around
artificial chromosomes in Xenopus egg extracts. Nature. 1996 Aug
1;382(6590):420-5. PubMed PMID: 8684481.

Heald R, McLoughlin M, McKeon F. Human wee1 maintains mitotic timing by
protecting the nucleus from cytoplasmically activated Cdc2 kinase. Cell. 1993 Aug
13;74(3):463-74. PubMed PMID: 8348613.

Heald R, McKeon F. Mutations of phosphorylation sites in lamin A that prevent
nuclear lamina disassembly in mitosis. Cell. 1990 May 18;61(4):579-89. PubMed
PMID: 2344612.