2018 to Present

Gibeaux R, Heald R. (2018) The use of Cell-Free Xenopus Extracts to Investigate Cytoplasmic Events. Cold Spring Harbor Protoc. [epub ahead of print]. PMID: 2990587

Heald R. (2018) A lab co-op helps young faculty members to thrive. Nature 556, 409. PMID: 29686370

Kitaoka M, Heald R, Gibeaux R. (2018) Spindle assembly in egg extracts of the Marsabit clawed frog, Xenopus borealis. Cytoskeleton (Hoboken) 75(6), 244-257. PMID: 29573195

Heald R and Gibeaux R. (2018) Subcellular scaling: does size matter for cell division? Curr Opin Cell Biol 52, 88-95. PMID: 29501026

Good MC, Heald R. (2018) Preparation of cellular extracts from Xenopus eggs and embryos. Cold Spring Harb Protoc. PMID: 29437998

Gibeaux R, Acker R, Kitaoka M, Georgiou G, van Kruijsbergen I, Ford B, Marcotte EM, Nomura DK, Kwon T, Veenstra GJC, Heald R. (2018) Paternal chromosome loss and metabolic crisis contribute to hybrid inviability in Xenopus. Nature 553, 337-341. PMID 29320479

2017

Elurbe DM, Paranjpe SS, Georgiou G, van Kruijsbergen I, Bogdanovic O, Gibeaux R, Heald R, Lister R, Huynen MA, van Heeringen SJ, Veenstra GJC. (2017) Regulatory remodeling in the allo-tetraploid frog Xenopus laevis. Genome Biol 18, 1335-7. PMID: 29065907. PMCID: PMC5655803 PDF

Sun M, Heald R. (2017) A DNA crosslinker collects mitotic chromosomes. Dev Cell 42, 440-442. PMID 28898675

2016

Session AM et al. (2016) Genome evolution in the allotetraploid frog Xenopus laevis. Nature 538, 336-43. PMID: 27762356. PMCID: PMC5313049 PDF

Grenfell AW, Strzelecka M, Heald R. (2016) Transcription brings the complex(ity) to the centromere. Cell Cycle 16 235-236. PMID: 27736298. PMCID: PMC5345185 PDF

Grenfell AW, Heald R, Strzelecka M. (2016) Mitotic noncoding RNA processing promotes kinetochore and spindle assembly in Xenopus. J Cell Biol 214, 133-41. PMID: 27402954. PMCID: PMC4949451PDF

Grenfell AW, Strzelecka M, Crowder ME, Helmke KJ, Schlaitz AL, Heald R. (2016) A versatile multivariate image analysis pipeline reveals features of Xenopus extract spindles. J Cell Biol 213, 127-36. PMID: 27044897. PMCID: PMC4828689 PDF

Janke C, Borisy G, Heald R, Howard J, Musacchio A, Nogales E. (2016) Viewpoint: Microtubules: 50 years on from the discovery of tubulin. Nat Rev Mol Cell Biol 17, 322-8. PMID: 27103327. PMCID: PMC5116387 PDF

2015

Heald R, Khodjakov A (2015) Thirty years of search and capture: The complex simplicity of mitotic spindle assembly. J Cell Biol 211, 1103-11. PMID: 26668328. PMCID: PMC4687881 PDF

Levy DL and Heald R. (2015) Biological scaling problems and solutions in Amphibians. Cold Spring Harb Perspect Biol 8(1):a019166. PMID: 26261280. PMCID: PMC4691792 PDF

Lane AB, Strzelecka M, Ettinger A, Grenfell AW, Wittmann T, Heald R. (2015) Enzymatically generated CRISPR libraries for genome labeling and screening. Dev Cell> 34, 373-8. PMID: 26212133. PMCID: PMC4536113 PDF

Crowder ME, Strzelecka M, Wilbur JD, Good MC, von Dassow G, Heald R. (2015) A comparative analysis of spindle morphometrics across metazoans. Curr Biol 25, 1542-50. PMID: 26004761. PMCID: PMC4464779 PDF

Miller KE, Heald R. (2015) Glutamylation of Nap1 modulates histone H1 dynamics and chromosome condensation in Xenopus. J Cell Biol 209, 211-20. PMID: 25897082. PMCID: PMC4411273 PDF

2014

Strzelecka M, Heald R. (2014) RUVs drive chromosome decondensation after mitosis. Dev Cell 31, 259-60. PMID: 25453825 PDF

Helmke KJ, Heald R. (2014) TPX2 levels modulate meiotic spindle size and architecture in Xenopus egg extracts. J Cell Biol 206, 385-93. PMID: 25070954. PMCID: PMC4121975 PDF

Heald R, Cohen-Fix O. (2014) Morphology and function of membrane-bound organelles. Curr Opin Cell Biol 26, 79-86. PMID: 24529249. PMCID: PMC3927147 PDF

2013

Good MC, Vahey MD, Skandarajah A, Fletcher DA, Heald R. (2013) Cytoplasmic volume modulates spindle size during embryogenesis. Science 342, 856-60. PMID: 24233724. PMCID: PMC4094345 PDF

Schlaitz A-L, Thompson J, Wong CCL, Yates JR, Heald R. (2013) REEP3/4 ensure endoplasmic reticulum clearance from metaphase chromatin and proper nuclear envelope architecture. Dev Cell 26, 316-323. PMID: 23911198. PMCID: PMC3745822 PDF

Wilbur JD, Heald R. (2013) Cryptic no longer: arrays of CLASP1 TOG domains. Structure 21, 869-70. PMID: 23747108 PDF

Bird SL, Heald R, Weis K. (2013) RanGTP and CLASP1 cooperate to position the mitotic spindle. Mol Biol Cell 24, 2506-14. PMID: 23783028. PMCID: PMC3744954 PDF

Helmke KJ, Heald R, Wilbur JD. (2013) Interplay between spindle architecture and function. Int Rev Cell Mol Biol 306, 83-125. PMID: 24016524

Wilbur J, Heald R. (2013) Mitotic spindle scaling during Xenopus development by kif2a and importin α. eLife 2:e00290. PMID: 23425906. PMCID: PMC3576809 PDF

Whitehead E, Heald R, Wilbur J. (2013) N-terminal phosphorylation of p60 katanin directly regulates microtubule severing. J Mol BiolJ Mol Biol 425, 214-221. PMID: 23178168. PMCID: PMC3540178 PDF

2012

Xiao B, Freedman BS, Miller KE, Heald R, Marko JF (2012) Histone H1 compacts DNA under force and during chromatin assembly. Mol Biol Cell 23, 4864-71. PMID: 23097493. PMCID: PMC3521692 PDF

Levy DL, Heald R. (2012) Mechanisms of intracellular scaling. Annu Rev Cell Dev Biol 28, 113-35. PMID: 22804576

Riggs B, Bergman ZJ, Heald R. (2012). Altering membrane topology with Sar1 does not impair spindle assembly in Xenopus egg extracts. Cytoskeleton 69, 591-9. PMID: 22605651. PMCID: PMC3661297 PDF

Patel K, Nogales E, Heald R. (2012) Multiple domains of human CLASP contribute to microtubule dynamics and organization in vitro and in Xenopus egg extracts. Cytoskeleton 69, 155-165. PMID: 22278908. PMCID: PMC3315288 PDF

2011

Loughlin R, Wilbur JD, McNally F, Nedelec F, Heald R. (2011) Katanin contributes to interspecies spindle length scaling in Xenopus. Cell 147, 1937- 1407. PMID: 22153081. PMCID: PMC3240848 PDF

Halpin D, Kalab P, Wang J, Weis K, Heald R. (2011) Spindle assembly around RCC1 coated beads in Xenopus egg extracts. PLoS Biol 9, e1001225. PMID: 22215983. PMCID: PMC3246454 PDF

Kieserman EK, Heald R. (2011) Mitotic chromosome size scaling in Xenopus. Cell Cycle 10, 3863-70. PMID: 22071695. PMCID: PMC3266116 PDF

Soderholm JF, Bird S, Kalab P, Sampathkumar Y, Hasegawa K, Uehara-Bingen M, Weis K, Heald R. (2011) Importazole, a small molecule inhibitor of the transport receptor receptor importin-beta. ACS Chem Biol 6, 700-708. PMID: 21469738. PMCID: PMC3137676 PDF

Fu H, Freedman BS, Lim CT, Heald R, Yan J. (2011) Atomic force microscope imaging of chromatin assembled in Xenopus egg extract. Chromosoma 120, 245-254. PMID: 21369955. PMCID: PMC3464096 PDF

Ridley A, Heald R. Cell Structure and Dynamics. (2011) Curr Opin Cell Biol 23, 1-3. PMID: 21190823

2010

Loughlin R, Heald R, Nedelec F. (2010) A computational model predicts Xenopus meiotic spindle organization. J Cell Biol 191, 1239-1249. PMID: 21173114. PMCID: PMC3010074 PDF

Levy DL, Heald R. (2010) Nuclear size is regulated by importin alpha and NTF2 in Xenopus. Cell 143, 288-298. PMID: 20946986. PMCID: PMC2966892PDF

Freedman BS, Miller KE, Heald R. (2010) Xenopus egg extracts increase dynamics of histone H1 on sperm chromatin. PLoS One 5(9). pii: e13111. PMID: 20927327. PMCID: PMC2947519PDF

Freedman BS, Heald R. (2010) Functional comparison of H1 histones in Xenopus reveals isoform-specific regulation by Cdk1 and RanGTP. Curr Biol 20, 1048-1052. PMID: 20471264. PMCID: PMC2902237 PDF

2009

Heald R, Walczak CE. (2009) Mitotic spindle assembly mechanisms. Chapter of book entitled "The Kinetochore: From Molecular Discoveries to Cancer Therapy", edited by Peter DeWulf and William Earnshaw. Springer Science.

Srsen V, Fant X, Heald R, Rabouille C, Merdes A. (2009) Centrosome proteins form and insoluble pericentriolar matrix during muscle cell differentiation. BMC Cell Biol 10:28. PMID: 19383121. PMCID: PMC2676252 PDF

2008

Heald R. (2008) Rebecca Heald: passionate about cycles. Interview by Caitlin Sedwick. J Cell Biol 570-1. PMID: 19015313. PMCID: PMC2582880 PDF

Loughlin R, Riggs B, Heald R. (2008) Snapshot: motor proteins in spindle assembly. Cell 134, 548. PMID: 18692476 PDF

Kalab P, Heald R. (2008) The RanGTP gradient – A GPS for the mitotic spindle. J. Cell Sci 121, 1577-1586. PMID: 18469014 PDF

Andersen CB, Wan Y, Chang, JW, Riggs B, Lee C, Liu Y, Sessa F, Villa F, Kwiatkowski N, Suzuki M, Nallan L, Heald R, Musacchio A, Gray NS. (2008) Discovery of selective aminothiozole aurora kinase inhibitors. ACS Chem Biol 3, 180-192. PMID: 18307303

Walczak CE, Heald R. (2008) Mechanisms of mitotic spindle assembly and function. Int Rev Cytol 265, 111-158. PMID: 18275887

2007

Blower MD, Feric E, Weis K, Heald R. (2007) Genome-wide analysis demonstrates conserved localization of mRNAs to mitotic microtubules. J Cell Biol 179, 1365-1373. PMID: 18166649. PMCID: PMC2373496 PDF

Heald R. (2007) Brinkley-fest of mitosis. Dev CellDev Cell 13, 168-176. PMID: 17681129 PDF

Brown KS, Blower MD, Maresca TJ, Grammer TC, Harland RM, Heald R. (2007) Xenopus tropicalis extracts provide insight into scaling of the mitotic spindle. J Cell Biol 176, 765-770. PMID: 17339377. PMCID: PMC2064050 PDF

Yan J, Maresca T, Skoko D, Adams CD, Xiao B, Christensen M, Heald R, Marko JF. (2007) Micromanipulation studies of chromatin fibers in Xenopus egg extracts reveal ATP-dependent nucleosome assembly dynamics. Mol Biol Cell 18, 464-474. PMID: 17108322. PMCID: PMC1783770 PDF

2006

Kenney RD, Heald R. (2006) Essential roles for cohesin in kinetochore and spindle function in Xenopus egg extracts. J Cell Sci 119, 5057-5066. PMID: 17158911 PDF

Kaláb P, Pralle A, Isacoff EY, Heald R, Weis K. (2006) Analysis of a RanGTP-regulated gradient essential for mitosis in somatic cells. Nature 440, 607-701. PMID: 16572176

Maresca TJ, Heald R. (2006) The long and the short of it: linker histone H1 is required for metaphase chromosome compaction. Cell Cycle 5, 589-91. PMID: 16582611

Soderholm J, Uehara-Bingen M, Weis K, Heald R. (2006) Challenges facing the biologist doing chemical genetics. Nat Chem Biol 2, 55-58. PMID: 16421579

Heald R. (2006) Cell biology: Serving up a plate of chromosomes. Science 311, 343-344. PMID: 16424328

Hannak E, Heald R. (2006) Xorbit/CLASP links dynamic microtubules to chromosomes in the Xenopus meiotic spindle. J Cell Biol 172, 19-25. PMID: 16390996. PMCID: PMC2063525 PDF

Budde PP, Desai A, Heald R. (2006) Analysis of microtubule polymerization in vitro and during the cell cycle in Xenopus egg extracts. Methods 38, 29-34. PMID: 16343934

Maresca TJ, Heald R. (2006) Methods for studying spindle assembly and chromosome condensation in Xenopus egg extracts Methods Mol Biol 322, 459-474. PMID: 16739744

Hannak E, Heald R. (2006) Investigating mitotic spindle assembly in vitro using Xenopus egg extracts. Nature Protocols 1, 2305-2314. PMID: 17406472

2005

Maresca TJ, Niederstrasser H, Weis K, Heald R. (2005) Xnf7 contributes to spindle integrity through its microtubule bundling activity. Curr Biol 15, 1755-61. PMID: 16213823 PDF

Maresca TJ, Heald R. (2005) Chromosome congression: another fine mesh we’ve gotten into. Dev Cell 9, 314-315. PMID: 16139222 PDF

Maresca TJ, Freedman BS, Heald R. (2005) Histone H1 is essential for mitotic chromosome architecture and segregation in Xenopus egg extracts. J Cell Biol 169, 859-869. PMID: 15967810. PMCID: PMC2171634 PDF

Blower MD, Nachury M, Heald R, Weis K. (2005). A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly. Cell 121, 223-234. PMID: 15851029 PDF

Soderholm J, Heald R. (2005) Scratch n' screen for inhibitors of cell migration. Chem Biol 12, 263-265. PMID: 15797209 PDF

Antonio C, Heald R, Vernos I. (2005). In vitro assays for mitotic spindle assembly and function. Cell Biology: A laboratory Handbook. J. Celis, ed. Vol. 2, Chapter 48, 379-386

Van Hooser A, Yuh P, Heald R. (2005) The perichromosomal layer. Chromosoma 114, 377-388. PMID: 16136320

2004

Banks JD, Heald R. (2004) Adenomatous polyposis coli associates with the microtubule-destabilizing protein XMCAK. Curr Biol 14(22), 2033-8. PMID: 15556866 PDF

Gadde S, Heald R. (2004) Mechanisms and molecules of the mitotic spindle. Curr Biol 14, R797-805. PMID: 15380094 PDF

Deehan R, Heald R. (2004) Centromere glue provides spindle cue. Cell 118, 529-30. PMID: 15339655 PDF

Skop AR, Liu H, Yates 3rd JR, Meyer BJ, Heald R. (2004) Dissection of the mammalian midbody proteome reveals conserved cytokinesis mechanisms. Science 305, 61-66. PMID: 15166316 . PMCID: PMC3679889 PDF

Krauss SW, Lee G, Chasis J, Mohandas N, Heald R. (2004) Two protein 4.1 domains essential for mitotic spindle and aster microtubule dynamics and organization in vitro. J Biol Chem 279, 25791-25798. PMID: 15102852 PDF

Heald R. (2004) Burning the spindle at both ends. Nature 427, 300-301. PMID: 14737150

Wignall SM, Gray NS, Chang Y-T, Juarez L, Jacob R, Burlingame A, Schultz PG, Heald R. (2004) Identification of a novel protein regulating microtubule stability through a chemical approach. Chem Biol 11, 135-46. PMID: 15113003 PDF

2003

Wignall SM, Heald R. (2003) The role of chromosome architecture in spindle assembly and anaphase: the condensed version. Cell Cycle 2, 590-1. PMID: 14512775

Krauss SW, Chen C, Penman S, Heald R. (2003) Nuclear actin and protein 4.1: essential interactions during nuclear assembly in vitro. Proc Natl Acad Sci USA 100, 10752-57. PMID: 12960380. PMCID: PMC196875 PDF

Wignall SM, Deehan R, Maresca TJ, Heald R. (2003) The condensin complex is required for proper spindle assembly and chromosome segregation in Xenopus extracts. J Cell Biol 161, 1041-51. PMID: 12821643. PMCID: PMC2172990 PDF

Budde PP, Heald R. (2003) Centrosomes and kinetochores, who needs 'em? The role of noncentromeric chromatin in spindle assembly. Curr Top Dev Biol 56, 85-113. PMID: 14584727

2002

Krauss SW, Heald R, Lee G, Nunomura W, Gimm JA, Mohandas N, Chasis JA. (2002) Two distinct domains of protein 4.1 critical for assembly of functional nuclei in vitro. J Biol Chem 277, 44339-46. PMID: 12171917 PDF

Kalab P, Weis K, Heald R. (2002) Visualization of a RanGTP gradient in interphase and mitotic Xenopus egg extracts. Science 295, 2452-56. PMID: 11923538 PDF

Heald R, Nogales E. (2002) Microtubule dynamics. J Cell Sci 115, 3-4. PMID: 11801717 PDF

2001

Chang YT, Wignall SM, Rosania GR, Gray NS, Hanson SR, Su AI, Merlie J Jr, Moon HS, Sangankar SB, Perez O, Heald R, Schultz PG. (2001) Synthesis and biological evaluation of myoseverin derivatives: microtubule assembly inhibitors. J Med Chem. 44, 4497-4500. PMID: 11741468.

Van Hooser A, Heald R. (2001) Kinetochore function: The complications of becoming attached. Curr Biol 11, R855-R857. PMID: 11696340 PDF

Merlie J Jr, Heald R. (2001) Mitotic spindle assembly in vitro. Curr Protoc Cell Biol Chapter 11, Unit 11.13. PMID: 18228304

Budde PP, Kumagai A, Dunphy WG, Heald R. (2001) Regulation of Op18 during spindle assembly in Xenopus egg extracts. J Cell Biol 153, 149-57. PMID: 11285281. PMCID: PMC2185528 PDF

Banks JD, Heald R. (2001) Chromosome movement: Dynein-out at the kinetochore. Curr Biol 11, R128-R131. PMID: 11250166 PDF

Nachury MV, Maresca TJ, Salmon WC, Waterman-Storer CM, Heald R, Weis K. (2001) Importin-beta is a mitotic target of the small GTPase Ran in spindle assembly. Cell 104, 95-106. PMID: 11163243 PDF

Wignall S, Heald R. (2001) Methods for the study of centrosome-independent spindle assembly in Xenopus egg extracts. Meth Cell Biol. 67, 241-256. PMID: 11550472

2000

Heald R. (2000) Motor function in the mitotic spindle. Cell 102, 399-402. PMID: 10966101 PDF

Merdes A, Heald R, Samejima K, Earnshaw WC, Cleveland DW. (2000) Formation of spindle poles by dynein/dynactin-dependent transport of NuMA. J Cell Biol 149, 851-62. PMID: 10811826. PMCID: PMC2174573 PDF

Heald R. (2000) A dynamic duo of microtubule modulators. Nature Cell Biol 2, E11-E12. PMID: 10620815

Heald R, Weis K. (2000) Spindles get the Ran around. Trends Cell Biol 10, 1-4. PMID: 10603469 PDF

1999

Rosania G, Merlie J, Gray N, Chang Y-T, Schultz PG, Heald R. (1999) A cyclin-dependent kinase inhibitor inducing cancer cell differentiation: Biochemical identification using Xenopus egg extracts. Proc Natl Acad Sci USA 96, 4797-4802. PMID: 10220373. PMCID: PMC21771 PDF

Heald R, Walczak CE. (1999) Microtubule-based motor function in mitosis. Curr Opin Struct Biol 9, 268-274. PMID: 10322211

1998

Walczak CE, Vernos I, Mitchison TJ, Karsenti E, Heald R. (1998) A model for the proposed roles of different microtubule-based motor proteins in establishing spindle bipolarity. Curr Biol 8, 903-913. PMID: 9707401 PDF