Preparation of CSF-Arrested Xenopus Egg Extracts

Preparation of Margaritas

Buffers and Reagents	fresh limes: approximately 40 depending on size and juiciness
(for 20-25 people)	1 liter Triple Sec
	1.5 - 1.75 liters Tequila
	7 lbs. Crushed ice "crystal clear"
	Salt
Equipment	Electric juicer, juice press, or eager rotation students
	4 liter beaker
	1 liter graduated cylinder
	Sterile pipette (5 or 10 ml)
	2 plates
	Glass or plastic cups
Protocol	1) Slice limes in half and squeeze out the juice. Measure 1 liter in graduated cylinder. Discard rinds.
	2) Combine in 4 liter receptacle: 1 part lime juice 1 part triple sec 1.5 parts tequila (note: if your tequila is high quality you may want to use a higher proportion; up to 2 parts.)
	3) Mix well with sterile pipette.
	4) Salt glass rims by turning upside down and placing on a plate of lime juice, then on a plate of salt. Omit salt for persons with high blood pressure or excessive age. Fill glasses with ice.
	5) Pour margaritas into glasses.
	6) Drink and enjoy.
	7) Do not drive home for at least 2 hours.