We are using Xenopus egg extracts to dissect the molecular mechanisms behind chromatin-dependent microtubule polymerization and organization. We are evaluating the roles of RNA, candidate chromatin factors and downstream microtubule effectors, including microtubule-associated proteins and motors, in spindle assembly, and developing pure component assays directed towards molecular reconstitution.
Organelle Scaling and Size Control
We are using several systems to understand how intracellular structures adapt to changes in cell size across species and within an organsim over development. Specifically, we are interested in understanding the physical and biochemical mechanisms that regulate spindle and chromosome size control and scaling.
We are using various wildtype and hybrid frog species to understand how size is controlled at the organismal, cellular, and genomic level.