Welcome to MCB 150L : : The objective of this course is to provide an introduction to experimental design and basic techniques commonly used in immunology research laboratories. The course is intended for upper-division undergraduate students majoring in immunology and those majoring in other biological sciences who plan to use immunological techniques in independent-study projects in the research laboratories or in employment.

The module on immunochemistry and cell culture provides experience in preparation of cell suspensions from lymphoid organs of mice, an assay for detection of activated T cells, production of monoclonal antibodies, enzyme-linked immunosorbent assay, immunoprecipitation, SDS-PAGE, western blot and staining of cells for flow cytometry.

The molecular module provides experience in isolation of DNA, Southern blotting, restriction mapping, subcloning, and DNA sequencing. Emphasis will be placed on experimental design and the interpretation of data.

A working knowledge of basic immunology is required; either previous completion of an upper-division course in immunology or concurrent enrollment in MCB 150 is sufficient. The laboratory work will include making and working with cell suspensions from spleens and thymuses of mice that have been euthanized with CO2 by the staff immediately before the laboratory period. It is not possible to immunize cell lines; cells from normal or immunized mice must be used for experiments that involve production of antibody responses in tissue culture. Therefore, this course is not suitable for students who object to the use of animals in teaching and/or research.

Class : :

Monday & Wednesday 1:00-5:30 PM
4054 VLSB
4 Units


Announcements : :

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