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Dernburg Lab

University of California, Berkeley

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Research Interests

Our group investigates chromosome organization and dynamics. We focus on meiosis, the specialized cell division process that gives rise to reproductive cells such as sperm, eggs, pollen, and spores. Meiotic errors underlie many human birth defects such as Down Syndrome, and also contribute to human infertility, especially in older women. Successful meiosis requires a unique series of chromosome interactions: each chromosome must pair with its homologous partner, and these paired chromosomes then exchange genetic information through homologous recombination. Crossover recombination gives rise to genetic diversity, and also creates physical links between chromosomes that enable them to segregate away from each other. We investigate these mechanisms using the nematode Caenorhabditis elegans as our primary model organism. This experimental system has enormous experimental advantages, including rapid and powerful genetics, robust genome editing, outstanding cytology, and the opportunity to directly observe meiosis through in vivo imaging. We are also studying the evolution and plasticity of meiosis by comparing these events in C. elegans to other nematodes, such as Pristionchus pacificus.

Chromosome architecture and dynamics

During meiosis chromosomes undergo remarkable and dynamic reorganization. A hallmark of meiotic entry is the reorganization of each replicated chromosome into a linear array of loops anchored to a central axis, which regulates many aspects of meiosis. We have characterized the molecular organization and function of chromosome axes through diverse biochemical and structural approaches, including crystallography (in collaboration with the laboratory of Kevin Corbett, UCSD/LICR) and superresolution microscopy (with Michal Wojcik and Ke Xu, UC Berkeley). Meiotic chromosomes also interact with molecular motors in the cytoplasm via a "LINC" complex, which enables them to move rapidly along the nuclear surface. By directly imaging these movements in living animals, we learned how they accelerate chromosomes' ability to find their partners and regulate their interactions with other chromosomes. In many organisms this movement is mediated by telomeres, the special repetitive DNA sequences at the ends of chromosomes, but in C. elegans this role has been acquired by "pairing centers," broad regions near one end of each chromosome that span hundreds of kilobases. We defined the molecular requirements for pairing center function and continue to investigate their roles in meiotic dynamics and regulation.

Phase separation and crossover signaling

A longstanding mystery about meiosis is how each pair of chromosomes undergoes at least one crossover, while the total number of crossovers is usually quite low. Indeed, in C. elegans, as in many other species, one and only one crossover occurs between each pair of homologs. Recent evidence has indicated that the synaptonemal complex (SC), a special polymer that assembles between paired chromosomes, plays an important role in this crossover control. Through live imaging in C. elegans, we discovered that the SC behaves as a unique, liquid crystalline compartment. We are exploring how this unusual material self-assembles through phase separation, and how it regulates meiotic recombination. We have identified biochemical signals and a conformational switch within this compartment that regulate crossover formation. Insights into this compartmentalized signaling mechanism are illuminating how cells make a variety of switch-like, spatially localized decisions.


Meiosis underlies the evolution of eukaryotes, and is also shaped by evolution, showing fascinating variation in the details of its execution. For example, in many organisms the process of meiotic homolog pairing depends on recombination machinery, but C. elegans is among the known exceptions to this rule. To better understand how evolution can rewire meiotic regulation, we are developing another nematode known as Pristionchus pacificus, an emerging satellite model organism, for molecular analysis of meiosis. By comparing how this process works in two different nematodes (C. elegans and P. pacificus), we are uncovering core aspects of meiotic regulation that are shared across eukaryotes, and exploring how novel mechanisms such as recombination-independent pairing have emerged in specific lineages.

Tool development

New insights in biology are driven by novel technologies. In particular, advances in fluorescence microscopy, DNA sequencing, genome editing, and mass spectrometry have enabled our lab to answer longstanding questions. Future advances will continue to influence our research directions. While our research group focuses on hypothesis-driven projects rather than methods development, we also work to expand our experimental toolbox through collaboration and innovation. For example, we developed methods for long-term in vivo imaging of adult C. elegans, which has enabled us to probe the dynamics of chromosome movement and synaptonemal complex assembly. We also adapted the auxin-inducible degradation (AID) system for use in C. elegans. This method enables rapid, robust protein depletion in response to an inexpensive, nontoxic small molecule, and also makes it possible to create more complex strains than we could using conventional loss-of-function alleles. We welcome potential researchers who are interested in applying cutting-edge tools to the study of chromosome organization.

Lab Members

I am studying the mechanisms of chromosome pairing and synapsis during meiosis in Pristionchus pacificus. By comparing this process to C. elegans, I am illuminating the evolution of sexual reproduction across eukaryotic lineages. Renzo AdilardiRenzo Adilardi
Postdoctoral Researcher
I am investigating the role of NPP-21, a nuclear pore protein, and the causes and consequences of its reorganization during meiosis. Shyanne AmoyoShyanne Amoyo
Using the satellite model nematode Pristionchus pacificus, I’m exploring how meiotic recombination is achieved, how it is coupled to homolog synapsis, and how these pathways are rewired during evolution. Regina BohnRegina Bohn
My work is probing how germ cells switch from proliferation to meiotic entry, and how homolog chromosomes recognize each other during meiosis. To address these questions I am developing new methods to map chromatin and chromosome structure in the C. elegans germline. Gina CaldasGina Caldas
Damon Runyon Postdoctoral Fellow
Pew Latin American Fellow
I am interested in developing the Auxin Inducible Degradation (AID) system for Pristionchus pacificus through a variety of the available nematode genome editing tools, including CRISPR cas9 and the MiniMos system. Sabrina LinSabrina Lin
My goal is to understand how the interactions between chromosomes, the nucleoskeleton, and the cytoskeleton contribute to homologous synapsis during meiosis. Chenshu LiuChenshu Liu
Life Sciences Research Foundation Fellow
One of my primary aims is to keep the gears of the lab well oiled to enable our lab members to focus on the science. Hoang PhamHoang Pham
Lab Manager
Using live imaging techniques and analysis, I am investigating the signals that regulate meiotic recombination. Weston StaufferWeston Stauffer
Graduate Student
I am studying how cell cycle signals and the DNA damage response regulate chromosome architecture and meiotic recombination. Zhouliang YuZhouliang Yu
Postdoctoral Researcher
I am defining the molecular composition of the synaptonemal complex using proximity-labeling methods, and working to reconstitute this dynamic material in vitro. Fan WuFan Wu
Postdoctoral Researcher
By investigating the molecular mechanisms underlying crossover regulation, I hope to learn how these pathways contribute to chromosome mis-segregation and aneuploidy. Liangyu ZhangLiangyu Zhang
Postdoctoral Researcher

Selected Publications

Interested in joining us?

If you are interested in joining our research group, please write directly to Abby Dernburg. We welcome highly motivated postdoctoral scholars with expertise in cell biology, genome biology, biophysics, and affiliated fields. Most of our Ph.D. students come through the UC Berkeley graduate program in Molecular and Cell Biology, or the Biophysics Graduate Group. We encourage interested undergraduates to apply to the Undergraduate Research Apprenticeship Program, although we do occasionally take students through direct applications.