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Week 1A : Cell Responses, Dose
Responses, Binding
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Dyson S, Gurdon JB. The
interpretation of position in a morphogen gradient as revealed by
occupancy of activin receptors. Cell. 1998 May 15;93(4):557-68.
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Ferrell JE Jr. Tripping
the switch fantastic: how a protein kinase cascade can convert graded
inputs into switch-like outputs. Trends Biochem Sci. 1996
Dec;21(12):460-6.
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Week 1B : Kinase Pathways
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Sako, Y., Minoghchi, S., and Yanagida,
T. (2000).
Single-molecule imaging of EGFR signalling on the surface of living
cells. Nat Cell Biol 2, 168-172. |
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Bishop, A. C., Ubersax,
J. A., Petsch, D. T., Matheos, D. P.,
Gray, N. S., Blethrow, J., Shimizu, E., Tsien, J. Z., Schultz, P. G.,
Rose, M. D., Wood, J. L., Morgan, D. O., and Shokat, K. M., A
chemical switch for inhibitor-sensitive alleles of any protein kinase,
Nature, 407, 395-401. (2000). |
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Zhang X, Gureasko J, Shen K,
Cole PA, Kuriyan J. An allosteric mechanism for
activation of the kinase domain of epidermal growth factor receptor.
Cell. 2006 Jun 16;125(6):1137-49.
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Pawson T, Nash P. Assembly of
cell regulatory systems through protein interaction domains. Science.
2003 Apr 18;300(5618):445-52.
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Week 2A: Ras and Map kinase pathways
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White, M., Nicolette, C.,
Minden, A., Polverino, A., Van Aelst, L., Karin, M., and MH, W.
(1995). Multiple Ras functions can contribute to mammalian cell
transformation. Cell 80, 533-541. |
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Jacobs, D., Glossip, D.,
Xing, H., Muslin, A. J., and Kornfeld,
K. (1999). Multiple docking sites on substrate proteins form a
modular system that mediates recognition by ERK MAP kinase. Genes Dev
13, 163-75. |
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Ferrell, J. E., Jr. (1999).
Building a cellular switch: more lessons from a good egg. Bioessays 21,
866-70. |
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Week 2B: G protein coupled pathways
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Taussig, R., Iniguez-Lluhi,
J., and Gilman, A. (1993). Inhibition of adenylyl cyclase by Gi alpha.
Science 261, 218-221 |
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Iiri, T., Herzmark, P.,
Nakamoto, J., van Dop, C., and Bourne, H. (1994). Rapid GDP release
from Gs alpha in patients with gain and loss of endocrine function.
Nature 371, 164-168. |
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Gudermann, T., Schoneberg, T.,
and Schultz, G. (1997). Functional
and structural complexity of signal transduction via G-protein-coupled
receptors. Ann. Rev. Neurosci. 20, 399-427. |
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Week 3A: Cell shape and motility
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.Honda, A., Nogami, M.,
Yokozeki, T., Yamazaki, M., Nakamura,
H., Watanabe, H., Kawamoto, K., Nakayama, K., Morris, A. J., Frohman,
M. A., and Kanaho, Y. (1999). Phosphatidylinositol 4-phosphate
5-kinase alpha is a downstream effector of the small G protein ARF6 in
membrane ruffle formation. Cell 99, 521-532. |
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Shimada, Y., Gulli, M. P., and
Peter, M. (2000). Nuclear sequestration of the exchange factor Cdc24 by
Far1 regulates cell polarity during yeast mating. Nat Cell Biol 2,
117-24. |
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Kaibuchi, K., Kuroda, S., and
Amano, M., Regulation of the cytoskeleton and cell adhesion by the Rho
family GTPases in mammalian cells, Annu Rev Biochem, 68, 459-86 (1999). |
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Week 3B: Cell death and survival
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Brunet, A., Bonni, A., Zigmond, M.
J., Lin, M. Z., Juo, P., Hu,
L. S., Anderson, M. J., Arden, K. C., Blenis, J., and Greenberg, M. E.
(1999). Akt promotes cell survival by phosphorylating and
inhibiting a Forkhead transcription factor. Cell 96, 857-68. |
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Coleman, M. L., Sahai, E.
A., Yeo, M., Bosch, M., Dewar, A., and Olson, M. F., Membrane blebbing
during apoptosis results from
caspase-mediated activation of ROCK I, Nat Cell Biol, 3, 339-345.
(2001). |
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Jiang
X, Wang X. Cytochrome
C-mediated apoptosis.
Annu Rev Biochem. 2004;73:87-106. Review.
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Week 4A: Turning signals off -- GAP
proteins
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.Watson, N., Linder, M., Druey,
K., Kehrl, J., and Blumer, K. (1996). RGS family members:
GTPase-activating proteins for heterotrimeric G-protein a-subunits.
Nature 383, 172-175. |
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Saitoh, O., Kubo, Y.,
Miyatani, Y., Asano, T., and Nakata, H. (1997). RGS8 accelerates
G-protein-mediated modulation of K+ currents. Nature 390, 525-9. |
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Week 4B: Turning signals off --
Receptor mediated endocytosis
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Cao, T. T., Deacon, H. W., Reczek, D.,
Bretscher, A., and von Zastrow, M. (1999). A kinase-regulated
PDZ-domain interaction controls endocytic sorting of the
beta2-adrenergic receptor. Nature 401, 286-90 |
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Le Roy C, Wrana JL. Clathrin- and
non-clathrin-mediated endocytic regulation of cell signalling.
Nat Rev Mol Cell Biol. 2005 Feb;6(2):112-26.
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Week 5A: Role of Scaffolding
Proteins
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Scott, K., and Zuker, C. S.
(1998). Assembly of the Drosophila phototransduction cascade into a
signalling complex shapes elementary responses. Nature 395, 805-8. |
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Burack WR, Shaw AS.(2000) Signal
transduction: hanging on a scaffold. Curr Opin Cell Biol. 12:211-6.
Review. |
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Week 5B: Maintaining specificity --
Combinatorial codes
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Kretzschmar, M.,
Doody, J., and Massague, J. (1997). Opposing BMP
and EGF signalling pathways converge on the TGF-beta family mediator
Smad1. Nature 389, 618-22. |
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Kobrinsky, E.,
Mirshahi, T., Zhang, H., Jin, T., and Logothetis,
D. E., Receptor-mediated hydrolysis of plasma membrane messenger PIP2
leads to K+-current desensitization, Nat Cell Biol, 2, 507-14. (2000). |
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The exam will be distributed
the last week of class and due in ~10 days
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