Papers

2024

The Rickettsia actin-based motility effectors RickA and Sca2 contribute differently to cell-to-cell spread and pathogenicity.
Tran CJ, Zubair-Nizami ZB, Langohr IM, Welch MD
bioRxiv doi: https://doi.org/10.1101/2024.08.20.608656 (2024)
bioRxiv Abstract |

 

Cryo-electron tomography of stationary phase Burkholderia thailandensis.
Khanna K, Welch MD
MicroPubl Biol 10.17912/micropub.biology.001178 (2024)
PubMed Abstract | Full Text

 

Critical roles of Rickettsia parkeri outer membrane protein B (OmpB) in the tick host.
Tongluan N, Engström P, Jirakanwisal K, Langohr IM, Welch MD, Macaluso KR
Infect Immun doi: 10.1128/iai.00515-23. Online ahead of print. (2024)
PubMed Abstract | Full Text

 

2023

Manipulation of host cell plasma membranes by intracellular bacterial pathogens.
Kostow N, Welch MD
Curr Opin Microbiol 71: 102241 (2023)
PubMed Abstract | Full Text

 

FAZ assembly in bloodstream form Trypanosoma brucei requires kinesin KIN-E.
Albisetti AC, Douglas RL, Welch MD
Mol Biol Cell 34: ar103 (2023)
bioRxiv Abstract | PubMed Abstract | Full Text

 

2022

Plasma membrane protrusions mediate host cell-cell fusion induced by Burkholderia thailandensis.
Kostow N, Welch MD
Mol Biol Cell 33: ar70 (2022)
bioRxiv Abstract | PubMed Abstract | Full Text

 

A patatin-like phospholipase mediates Rickettsia parkeri escape from host membranes.
Borgo GM, Burke TP, Tran CJ, Lo NTN, Engström P, Welch MD
Nat Commun 13: 3656 (2022)
bioRxiv Abstract | PubMed Abstract | Full Text

 

A glycine-rich PE_PGRS protein governs mycobacterial actin-based motility.
Hill NS, Welch MD
Nat Commun 13: 3608 (2022)
bioRxiv Abstract | PubMed Abstract | Full Text

 

2021

Interferon receptor-deficient mice are susceptible to eschar-associated rickettsiosis.
Burke TP, Engström P, Tran CJ, Langohr IM, Glasner DR, Espinosa DA, Harris E, Welch MD
eLife 10: e67029 (2021)
bioRxiv Abstract | PubMed Abstract | Full Text

 

Baculovirus actin-rearrangement-inducing factor ARIF-1 induces the formation of dynamic invadosome clusters.
Lauko DI, Ohkawa T, Mares SE, Welch MD
Mol Biol Cell 32: 1433-1445 (2021)
bioRxiv Abstract | PubMed Abstract | Full Text

 

Lysine methylation shields an intracellular pathogen from ubiquitylation and autophagy.
Engström P, Burke TP, Tran CJ, Iavarone AT, Welch MD
Sci Adv 7: eabg2517 (2021)
bioRxiv Abstract | PubMed Abstract | Full Text

 

Mechanical competition triggered by innate immune signaling drives the collective extrusion of bacterially infected epithelial cells.
Bastounis EE, Serrano-Alcalde F, Radhakrishnan P, Engström P, Gómez-Benito MJ, Oswald MS, Yeh YT, Smith JG, Welch MD, García-Aznar JM, Theriot JA
Dev Cell 56: 443-460.e11 (2021)
PubMed Abstract | Full Text

 

2020

Trypanosomes have divergent kinesin-2 proteins that function differentially in flagellum biosynthesis and cell viability.
Douglas RL, Haltiwanger BM, Albisetti AA, Wu H, Jeng RL, Mancuso J, Cande WZ, Welch MD
J Cell Sci 133: jcs129213 (2020)
bioRxiv Abstract | PubMed Abstract | Full Text

 

Inflammasome-mediated antagonism of type I interferon enhances Rickettsia pathogenesis.
Burke TP, Engström P, Chavez RA, Fonbuena JA, Vance RE, Welch MD
Nat Microbiol 5: 688-696 (2020)
bioRxiv Abstract | PubMed Abstract | Full Text

 

2019

A metabolic dependency for host isoprenoids in the obligate intracellular pathogen Rickettsia parkeri underlies a sensitivity for the statin class of host-targeted therapeutics.
Ahyong V, Berdan CA, Burke TP, Nomura DK, Welch MD
mSphere 4: (6). pii: e00536-19. doi: 10.1128/mSphere.00536-19 (2019)
bioRxiv Abstract | PubMed Abstract | Full Text

 

Evasion of autophagy mediated by Rickettsia surface protein OmpB is critical for virulence.
Engström P, Burke TP, Mitchell G, Ingabire N, Mark KG, Golovkine G, Iavarone AT, Rape M, Cox JS, Welch MD
Nat Microbiol 4: 2538–2551 (2019)
PubMed Abstract | Full Text

 

2018

Baculovirus actin-based motility drives nuclear envelope disruption and nuclear egress.
Ohkawa T, Welch MD.
Curr Biol 28: 2153-2159.e4 (2018)
PubMed Abstract | Full Text

 

RECON-dependent inflammation in hepatocytes enhances Listeria monocytogenes cell-to-cell spread.
McFarland AP, Burke TP, Carletti AA, Glover RC, Tabakh H, Welch MD, Woodward JJ.
mBio 9: e00526-18 (2018)
PubMed Abstract | Full Text

 

The role of Sca2 and RickA in the dissemination of Rickettsia parkeri in Amblyomma maculatum.
Harris EK, Jirakanwisal K, Verhoeve VI, Fongsaran C, Welch MD, Macaluso KR.
Infect Immun 86: e00123-18 (2018)
PubMed Abstract | Full Text

 

A streamlined method for transposon mutagenesis of Rickettsia parkeri yields numerous mutations that impact infection.
Lamason RL, Kafai NM, Welch MD.
PLoS One 13: e0197012 (2018)
bioRxiv Abstract | PubMed Abstract | Full Text

 

Baculovirus AC102 is a nucleocapsid protein that is crucial for nuclear actin polymerization and nucleocapsid morphogenesis.
Hepp SE, Borgo GM, Ticau S, Ohkawa T, Welch MD.
J Virol 92: e00111-18 (2018)
bioRxiv Abstract | PubMed Abstract | Full Text

 

2017

Actin-based motility and cell-to-cell spread of bacterial pathogens.
Lamason RL, Welch MD.
Curr Opin Microbiol 38: 45-57 (2017)
PubMed Abstract | Full Text

 

2016

Actin-based motility of bacterial pathogens: mechanistic diversity and its impact on virulence.
Choe JE, Welch MD.
Pathog Dis 74: ftw099 (2016)
PubMed Abstract | Full Text

 

Rickettsia Sca4 reduces vinculin-mediated intercellular tension to promote spread.
Lamason RL, Bastounis E, Kafai NM, Serrano R, Del Álamo JC, Theriot JA, Welch MD.
Cell 167: 670-683.e10 (2016)
PubMed Abstract | Full Text

 

Rab1 recruits WHAMM during membrane remodeling but limits actin nucleation.
Russo AJ, Mathiowetz AJ, Hong S, Welch MD, Campellone KG.
Mol Biol Cell 27: 967-978 (2016)
PubMed Abstract | Full Text

 

2015

Why should cell biologists study microbial pathogens?
Welch MD
Mol Biol Cell 26: 4295-4301 (2015)
PubMed Abstract | Full Text

 

Virulent Burkholderia species mimic host actin polymerases to drive actin-based motility.
Benanti EL, Nguyen CM, Welch MD.
Cell 161: 348-360 (2015)
PubMed Abstract | Full Text

 

Cell migration, freshly squeezed.
Welch MD
Cell 160: 581-582 (2015)
PubMed Abstract | Full Text

 

2014

Rickettsia actin-based motility occurs in distinct phases mediated by different actin nucleators.
Reed SC, Lamason RL, Risca VI, Abernathy E, Welch MD.
Curr Biol 24: 98-103 (2014)
PubMed Abstract | Full Text

 

A 2-pyridone-amide inhibitor targets the glucose metabolism pathway of Chlamydia trachomatis.
Engström P, Krishnan KS, Ngyuen BD, Chorell E, Normark J, Silver J, Bastidas RJ, Welch MD, Hultgren SJ, Wolf-Watz H, Valdivia RH, Almqvist F, Bergström S
mBio 6: e02304-14 (2014)
PubMed Abstract | Full Text

 

Editorial overview: Cell architecture: Cellular organization and function.
Biggins S, Welch MD.
Curr Opin Cell Biol 26: v-vii (2014)
PubMed Abstract | Full Text

 

Electron tomography and simulation of baculovirus actin comet tails support a tethered filament model of pathogen propulsion.
Mueller J, Pfanzelter J, Winkler C, Narita A, Le Clainche C, Nemethova M, Carlier MF, Maeda Y, Welch MD, Ohkawa T, Schmeiser C, Resch GP, Small JV.
PLoS Biol 12: e1001765 (2014)
PubMed Abstract | Full Text

 

2013

Arp2/3-mediated actin-based motility: a tail of pathogen abuse.
Welch MD, Way M.
Cell Host Microbe 14: 242-255 (2013)
PubMed Abstract | Full Text

 

2012

Establishing intracellular infection: escape from the phagosome and intracellular colonization (Rickettsiaceae).
Welch MD, Reed SC, Haglund CM.
Chapter 5 of Intracellular Pathogens II: Rickettsiales, edited by G. H. Palmer and A. F. Azad (2012)
[ Request by emailing welch@berkeley.edu ]

 

Structural insights into WHAMM-mediated cytoskeletal coordination during membrane remodeling.
Shen QT, Hsiue PP, Sindelar CV, Welch MD, Campellone KG, Wang HW.
J Cell Biol 199: 111-124 (2012)
PubMed Abstract | Full Text

 

Regulation of integrin trafficking, cell adhesion, and cell migration by WASH and the Arp2/3 complex.
Duleh SN, Welch MD.
Cytoskeleton 69: 1047-1058 (2012)
PubMed Abstract | Full Text

 

Expression of an epitope-tagged virulence protein in Rickettsia parkeri using transposon insertion.
Welch MD, Reed SC, Lamason RL, Serio AW.
PLoS One 7: e37310 (2012)
PubMed Abstract | Full Text

 

The nuclear localization of actin requires AC102 in Autographa californica M nucleopolyhedrovirus-infected cells.
Gandhi KM, Ohkawa T, Welch MD, Volkman LE.
J Gen Virol 93: 1795-1803 (2012)
PubMed Abstract | Full Text

 

Membrane-deforming proteins play distinct roles in actin pedestal biogenesis by enterohemorrhagic E.coli.
Campellone KG, Siripala AD, Leong JM, Welch MD.
J Biol Chem 287: 20613-20624 (2012)
PubMed Abstract | Full Text

 

Rickettsia parkeri invasion of diverse host cells involves an Arp2/3 complex, WAVE complex and Rho-family GTPase-dependent pathway.
Reed SC, Serio AW, Welch MD.
Cell Microbiol 14: 529-545 (2012)
PubMed Abstract | Full Text

 

2011

Antibacterial autophagy occurs at PI(3)P-enriched domains of the endoplasmic reticulum and requires Rab1 GTPase.
Huang J, Birmingham CL, Shahnazari S, Shiu J, Zheng YT, Smith AC, Campellone KG, Heo WD, Gruenheid S, Meyer T, Welch MD, Ktistakis NT, Kim PK, Klionsky DJ, Brumell JH.
Autophagy 7: 17-26 (2011)
PubMed Abstract | Full Text

 

The actin nucleation factor JMY is a negative regulator of neuritogenesis.
Firat-Karalar EN, Hsiue PP, Welch MD.
Mol Biol Cell 22: 4563-4574 (2011)
PubMed Abstract | Full Text

 

Pathogens and polymers: Microbe-host interactions illuminate the cytoskeleton.
Haglund CM, Welch MD.
J Cell Biol 195: 7-17 (2011)
PubMed Abstract | Full Text

 

New mechanisms and functions of actin nucleation.
Firat-Karalar EN, Welch MD.
Curr Opin Cell Biol 23: 4-13 (2011)
PubMed Abstract | Full Text

 

2010

WASH and the Arp2/3 complex regulate endosome shape and trafficking.
Duleh SN, Welch MD.
Cytoskeleton 67: 193-206 (2010)
PubMed Abstract | Full Text

 

Rickettsia Sca2 is a bacterial formin-like mediator of actin-based motility.
Haglund CM, Choe JE, Skau CT, Kovar DR, Welch MD.
Nat Cell Biol 12: 1057-1063 (2010)
PubMed Abstract | Full Text

 

Actin-based motility drives baculovirus transit to the nucleus and cell surface.
Ohkawa T, Volkman LE, Welch MD.
J Cell Biol 190: 187-195 (2010)
PubMed Abstract | Full Text

 

Defining a core set of actin cytoskeletal proteins critical for actin-based motility of Rickettsia.
Serio AW, Jeng RL, Haglund CM, Reed SC, Welch MD.
Cell Host Microbe 7: 388-398 (2010)
PubMed Abstract | Full Text

 

An actin-filament-binding interface on the Arp2/3 complex is critical for nucleation and branch stability.
Goley ED, Rammohan A, Znameroski EA, Firat-Karalar EN, Sept D, Welch MD.
Proc Natl Acad Sci U S A 107: 8159-8164 (2010)
PubMed Abstract | Full Text

 

A nucleator arms race: cellular control of actin assembly.
Campellone KG, Welch MD
Nat Rev Mol Cell Biol 11: 237-251 (2010)
PubMed Abstract | Full Text

 

2008

WHAMM is an Arp2/3 complex activator that binds microtubules and functions in ER to Golgi transport.
Campellone KG, Webb NJ, Znameroski EA, Welch MD.
Cell 134: 148-161 (2008)
PubMed Abstract | Full Text

 

Repetitive N-WASP-binding elements of the enterohemorrhagic Escherichia coli effector EspF(U) synergistically activate actin assembly.
Campellone KG, Cheng HC, Robbins D, Siripala AD, McGhie EJ, Hayward RD, Welch MD, Rosen MK, Koronakis V, Leong JM.
PLoS Pathog 3: e1000191 (2008)
PubMed Abstract | Full Text

 

2007

Snapshot: Actin Regulators I.
Siripala AD, Welch MD.
Cell 128: 626 (2007)
PubMed Abstract | Full Text

 

Snapshot: Actin Regulators II.
Siripala AD, Welch MD.
Cell 128: 1014 (2007)
PubMed Abstract | Full Text

 

2006

A role for cortactin in Listeria monocytogenes invasion of NIH 3T3 cells, but not in its intracellular motility.
Barroso C, Rodenbusch SE, Welch MD, Drubin DG.
Cell Motil Cytoskeleton 63: 231-243 (2006)
PubMed Abstract | Full Text

 

Arp2/3 ATP hydrolysis-catalysed branch dissociation is critical for endocytic force generation.
Martin AC, Welch MD, Drubin DG.
Nat Cell Biol 8: 826-833 (2006)
PubMed Abstract | Full Text

 

The ARP2/3 complex: an actin nucleator comes of age.
Goley ED, Welch MD.
Nat Rev Mol Cell Biol 7: 713-726 (2006)
PubMed Abstract | Full Text

 

Dynamic nuclear actin assembly by Arp2/3 complex and a baculovirus WASP-like protein.
Goley ED, Ohkawa T, Mancuso J, Woodruff JB, D'Alessio JA, Cande WZ, Volkman LE, Welch MD.
Science 314: 464-467 (2006)
PubMed Abstract | Full Text

 

2005

Identification of a bacterial factor required for actin-based motility of Burkholderia pseudomallei.
Stevens MP, Stevens JM, Jeng RL, Taylor LA, Wood MW, Hawes P, Monaghan P, Welch MD, Galyov EE.
Mol Microbiol 56: 40-53 (2005)
PubMed Abstract | Full Text

 

Plasma membrane organization is essential for balancing competing pseudopod- and uropod-promoting signals during neutrophil polarization and migration.
Bodin S, Welch MD.
Mol Biol Cell 16: 5773-5783 (2005)
PubMed Abstract | Full Text

 

NMR analyses of the activation of the Arp2/3 complex by neuronal Wiskott-Aldrich syndrome protein.
Kreishman-Deitrick M, Goley ED, Burdine L, Denison C, Egile C, Li R, Murali N, Kodadek TJ, Welch MD, Rosen MK.
Biochemistry 44: 15247-15256 (2005)
PubMed Abstract | Full Text

 

Effects of Arp2 and Arp3 nucleotide-binding pocket mutations on Arp2/3 complex function.
Martin AC, Xu XP, Rouiller I, Kaksonen M, Sun Y, Belmont L, Volkmann N, Hanein D, Welch M, Drubin DG
J Cell Biol 168: 315-328 (2005)
PubMed Abstract | Full Text

 

Actin-based motility of intracellular pathogens.
Gouin E, Welch MD, Cossart P.
Curr Opin Microbiol 8: 35-45 (2005)
PubMed Abstract | Full Text

 

2004

A Rickettsia WASP-like protein activates the Arp2/3 complex and mediates actin-based motility.
Jeng RL, Goley ED, D'Alessio JA, Chaga OY, Svitkina TM, Borisy GG, Heinzen RA, Welch MD.
Cell Microbiol 6: 761-769 (2004)
PubMed Abstract | Full Text

 

Critical conformational changes in the Arp2/3 complex are induced by nucleotide and nucleation promoting factor.
Goley ED, Rodenbusch SE, Martin AC, Welch MD.
Mol Cell 16: 269-279 (2004)
PubMed Abstract | Full Text

 

2003

Mycobacterium marinum escapes from phagosomes and is propelled by actin-based motility.
Stamm LM, Morisaki JH, Gao LY, Jeng RL, McDonald KL, Roth R, Takeshita S, Heuser J, Welch MD, Brown EJ.
J Exp Med 198: 1361-1368 (2003)
PubMed Abstract | Full Text

 

Formation of filopodia-like bundles in vitro from a dendritic network.
Vignjevic D, Yarar D, Welch MD, Peloquin J, Svitkina T, Borisy GG.
J Cell Biol 160: 951-962 (2003)
PubMed Abstract | Full Text

 

Salmonella effectors translocated across the vacuolar membrane interact with the actin cytoskeleton.
Miao EA, Brittnacher M, Haraga A, Jeng RL, Welch MD, Miller SI.
Mol Microbiol 48: 401-415 (2003)
PubMed Abstract | Full Text

 

2002

Turning on the Arp2/3 complex at atomic resolution.
Borths EL, Welch MD.
Structure 10: 131-135 (2002)
PubMed Abstract | Full Text

 

Cellular control of actin nucleation.
Welch MD, Mullins RD.
Annu Rev Cell Dev Biol 18: 247-288 (2002)
PubMed Abstract | Full Text

 

Motility determinants in WASP family proteins.
Yarar D, D'Alessio JA, Jeng RL, Welch MD.
Mol Biol Cell 13: 4045-4059 (2002)
PubMed Abstract | Full Text

 

2001

Systematic mutational analysis of the amino-terminal domain of ActA reveals novel functions in actin-based motility.
Lauer P, Theriot JA, Skoble J, Welch MD, Portnoy DA.
Mol Microbiol 42: 1163-1177 (2001)
PubMed Abstract | Full Text

 

Cytoskeleton: actin and endocytosis -- no longer the weakest link.
Jeng RL, Welch MD.
Curr Biol 9: R691-R694 (2001)
PubMed Abstract | Full Text

 

Pivotal role of VASP in Arp2/3 complex-mediated actin nucleation, actin branch-formation, and Listeria monocytogenes motility.
Skoble J, Auerbuch V, Goley ED, Welch MD, Portnoy DA.
J Cell Biol 155: 89-100 (2001)
PubMed Abstract | Full Text

 

Reconstitution of human Arp2/3 complex reveals critical roles for individual subunits in complex structure and activity.
Gournier H, Goley ED, Niederstrasser H, Trinh T, Welch MD.
Mol Cell 8: 1041-1052 (2001)
PubMed Abstract | Full Text

 

2000

Three regions within ActA promote Arp2/3 complex-mediated actin nucleation and Listeria monocytogenes motility.
Skoble J, Portnoy DA, Welch MD.
J Cell Biol 150: 527-537 (2000)
PubMed Abstract | Full Text

 

1999

The Wiskott-Aldrich syndrome protein directs actin-based motility by stimulating actin nucleation with the Arp2/3 complex.
Yarar D, To W, Abo A, Welch MD.
Curr Biol 9: 555-558 (1999)
PubMed Abstract | Full Text

 

The world according to Arp: regulation of actin nucleation by the Arp2/3 complex.
Welch MD.
Trends Cell Biol 9: 423-427 (1999)
PubMed Abstract | Full Text

 

1998

Interaction of human Arp2/3 complex and the Listeria monocytogenes ActA protein in actin filament nucleation.
Welch MD, Rosenblatt J, Skoble J, Portnoy DA, Mitchison TJ.
Science 281: 105-108 (1998)
PubMed Abstract | Full Text

 

1997

Actin polymerization is induced by Arp2/3 protein complex at the surface of Listeria monocytogenes.
Welch MD, Iwamatsu A, Mitchison TJ.
Nature 385: 265-269 (1997)
PubMed Abstract | Full Text

 

The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly.
Welch MD, DePace AH, Verma S, Iwamatsu A, Mitchison TJ.
J Cell Biol 138: 375-384 (1997)
PubMed Abstract | Full Text