10X TBE BUFFER

3 Liters
324 g Tris base
165g Boric acid
120mls 0.5M EDTA (pH 8.0)
autoclave for 20 min

2 Liters
216g Tris base
110g Boric acid
80mls 0.5M EDTA (pH 8.0)
autoclave for 20 min

1 Liters
108g Tris base
55g Boric acid
40mls 0.5M EDTA (pH 8.0)
autoclave for 20 min