CARBONATE WESTERN TRANSFER SOLUTIONS
Dunn, Anal. Biochem. 1986: 157
|
1.5L |
|
1.0L
|
10mM NaHCO3 |
1.26g |
chill to 4oC |
0.84g
|
3mM Na2CO3 |
0.477g |
pH ~9.9 |
0.318g
|
in 20% MeOH |
300mL |
|
200mL
|
- Blot: no need to presoak gell.
Transblot: 1-2H @ 1A keep T<35oC
Miniblot: 1H @ 250mA
- Assemble wet sandwich on black side:
black side holder
brillo pad
3MM paper
gel
nitrocellulose
3MM
brillo
white side holder
|