300 ml 2% agar
100 ml 4X M9
4 ml 20% dextrose
4 ml 0.1M MgSO₄
0.1 ml B1 (0.1%)
4 ml Threonine (100X Stock 4 mg/ml)
4 ml Proline (100X Stock 4 mg/ml)

4XM9

56 gm. Na₂HPO₄ (Anhydrous)
24 gm. KH₂PO₄ (Anhydrous)
8 gm. NH₄Cl
4 gm. NaCl
Bring to 2 liters with dH₂O.
Aliquot 400 ml / bottle and autoclave.