L-BROTH

30g tryptone
15g yeast extract
15g NaCl (sodium chloride)

1. bring H₂O up to ~2700ml and stir
   
2. adjust pH to 7.4 with NaOH (sodium hydroxide)
   
3. bring H₂O up to 3l; stir to mix
   
4. pour into 1l bottles and autoclave 20minutes
   

L-BROTH Amp plates

1. Make up solution as above
   
2. after adjusting pH add 45g agar
   
3. bring H₂O to 3l
   
4. autoclave 30 minutes
   
5. cool to about 65^oC and add 0.15g of ampicillin
   
6. mix and pour plates
   

note:
kanamycin sulfate
1ml 50mg/mL per Liter