1) 200ml 2X YEP 2) 50 ml 20% dextrose 3) 100 mg Geneticin (G418 sulfate, Invitrogen Gibco 11811-031 4) Mix 2, 3 and 4 5) Melt 1 bottle of 4% agar (250ml) in microwave 6) Mix 4 and 5 and pour.