G418 Dropout (final conc. G418 - 200 µg/ml

1) Dissolve in 200 ml of H₂0 and sterilize
     0.85 g yeast nitrogen base without (NH₄)₂SO₄ or aa
     0.5 g monosodium glutamate
     1.0 g droupout powder
     100 mg G418 (G418 sulfate, Invitrogen Gibco 11811-031)

2) 50 ml 20% dextrose
3) Mix 1 and 2
4) add 250 ml 4% agar