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Clonat PLATES (500ml) | ||
Clonat plates (final conc.Clonat - 100 µg/ml)
1) 200ml 2X YEP 2) 50 ml 20% dextros 3) 250 µl of 200 mg/ml clonat stock solution* 4) Mix 2, 3 and 4 5) Melt 1 bottle of 4% agar (250ml) in microwave 6) Mix 4 and 5 and pour. |
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Comments: stock solution 200 mg/ml in H20. Store frozen. Clonat from Werner BioAgents. | ||
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