Laboratory  of  John G. Forte  

Department of Cell & Molecular Biology,  University of California, Berkeley
 
Selected publications:

Nakada SL, Crothers JM Jr, Machen TE, Forte JG.  Apical vacuole formation by gastric parietal cells in primary culture: effect of low extracellular Ca2+.  Am J Physiol Cell Physiol. 2012 Oct 24. [Epub ahead of print] {PubMed Link}

He W, Liu W, Chew CS, Baker SS, Baker RD, Forte JG, Zhu L.  Acid secretion-associated translocation of KCNJ15 in gastric parietal cells.  Am J Physiol Gastrointest Liver Physiol. 2011 Oct;301(4):G591-600 {PubMed Link}

Zhu L, Crothers J Jr, Zhou RH, Forte JG.  A Possible Mechanism for Ezrin to Establish Epithelial Cell Polarity.  Am J Physiol Cell Physiol. 2010 Aug;299(2):C431-43. {PubMed Link}

Forte JG, Zhu L.  Apical recycling of the gastric parietal cell H,K-ATPase.  Annu Rev Physiol. 2010 Mar 17;72:273-96. {PubMed Link}

Zhu L, Hatakeyama J, Zhang B, Makdisi J, Ender C, Forte JG.  Novel insights of the gastric gland organization revealed by chief cell specific expression of moesin.  Am J Physiol Gastrointest Liver Physiol. 2008 Dec 12; {PubMed Link}

Zhu L, Hatakeyama J, Chen C, Shastri A, Poon K, Forte JG.  Comparative study of ezrin phosphorylation among different tissues: more is good; too much is bad.  Am J Physiol Cell Physiol. 2008 Jul;295(1):C192-202. {PubMed Link}

Reenstra WW, Crothers J Jr, Forte JG.  The Conformation of H,K-ATPase Determines the Nucleoside Triphosphate (NTP) Selectivity for Active Proton Transport.  Biochemistry. 2007 Sep 4;46(35):10145-52. {PubMed Link}

Mettler SE, Ghayouri S, Christensen GP, Forte JG.  Modulatory Role of Phosphoinositide 3-Kinase (PI3K) in Gastric Acid Secretion.  Am J Physiol Gastrointest Liver Physiol. 2007 Jun 14; {PubMed Link}

Zhu L, Zhou R, Mettler S, Wu T, Abbas A, Delaney J, Forte JG.  High turnover of ezrin T567 phosphorylation: conformation, activity and cellular function.  Am J Physiol Cell Physiol. 2007 Jun 6; {PubMed Link}

Zhou R, Zhu L, Kodani A, Hauser P, Yao X, Forte JG.  Phosphorylation of ezrin on threonine 567 produces a change in secretory phenotype and repolarizes the gastric parietal cell.  J Cell Sci. 2005 Oct 1;118(Pt 19):4381-91. {PubMed Link}

Karvar S, Zhu L, Crothers J Jr, Wong W, Turkoz M, Forte JG.  Cellular localization and stimulation-associated distribution dynamics of syntaxin-1 and syntaxin-3 in gastric parietal cells.  Traffic. 2005 Aug;6(8):654-66. {PubMed Link}

Zhu L, Liu Y, Forte JG.  Ezrin oligomers are the membrane-bound dormant form in gastric parietal cells.  Am J Physiol Cell Physiol. 2005 Jun;288(6):C1242-54. {PubMed Link}

Crothers JM Jr, Asano S, Kimura T, Yoshida A, Wong A, Kang JW, Forte JG.  Contribution of oligosaccharides to protection of the H,K-ATPase β-subunit against trypsinolysis.  Electrophoresis. 2004 Aug;25(15):2586-92. {PubMed Link}

Duman JG, Lee E, Lee GY, Singh G, Forte JG.  Membrane fusion correlates with surface charge in exocytic vesicles.  Biochemistry. 2004 Jun 22;43(24):7924-39. {PubMed Link}

Sawaguchi A, McDonald KL, Forte JG.  High-pressure freezing of isolated gastric glands provides new insight into the fine structure and subcellular localization of H+/K+-ATPase in gastric parietal cells.  J Histochem Cytochem. 2004 Jan;52(1):77-86. {PubMed Link}

Zhou R, Cao X, Watson C, Miao Y, Guo Z, Forte JG, Yao X.  Characterization of protein kinase A-mediated phosphorylation of ezrin in gastric parietal cell activation.  J Biol Chem. 2003 Sep 12;278(37):35651-9. {PubMed Link}

Karvar S, Yao X, Crothers JM Jr, Liu Y, Forte JG.  Localization and function of soluble N-ethylmaleimide-sensitive factor attachment protein-25 and vesicle-associated membrane protein-2 in functioning gastric parietal cells.  J Biol Chem. 2002 Dec 20;277(51):50030-5. {PubMed Link}

Sawaguchi A, McDonald KL, Karvar S, Forte JG.  A new approach for high-pressure freezing of primary culture cells: the fine structure and stimulation-associated transformation of cultured rabbit gastric parietal cells.  J Microsc. 2002 Dec;208(Pt 3):158-66. {PubMed Link}

Yao X, Forte JG.  Cell biology of acid secretion by the parietal cell.  Annu Rev Physiol. 2003;65:103-31. Review. {PubMed Link}

Rong Q, Bastaki SM, Forte JG.  An improved method to evaluate secretory activity of isolated gastric glands and cells.  Dig Dis Sci. 2002 May;47(5):1001-7. {PubMed Link}

Duman JG, Singh G, Lee GY, Machen TE, Forte JG.  Ca2+ and Mg2+/ATP independently trigger homotypic membrane fusion in gastric secretory membranes.  Traffic. 2002 Mar;3(3):203-17. {PubMed Link}

Duman JG, Pathak NJ, Ladinsky MS, McDonald KL, Forte JG.  Three-dimensional reconstruction of cytoplasmic membrane networks in parietal cells. J Cell Sci. 2002 Mar 15;115(Pt 6):1251-8. {PubMed Link}

Ammar DA, Zhou R, Forte JG, Yao X.  Syntaxin 3 is required for cAMP-induced acid secretion: streptolysin O-permeabilized gastric gland model. Am J Physiol Gastrointest Liver Physiol. 2002 Jan; 282(Pt 1):G23-33. {PubMed Link}

Okamoto CT, Duman JG, Tyagarajan K, McDonald KL, Jeng YY, McKinney J, Forte TM, Forte JG.  Clathrin in gastric acid secretory (parietal) cells: biochemical characterization and subcellular localization. Am J Physiol Cell Physiol. 2000 Sep;279(3):C833-51. {PubMed Link}

Duman JG, Tyagarajan K, Kolsi MS, Moore HP, Forte JG.  Expression of rab11a N124I in gastric parietal cells inhibits stimulatory recruitment of the H+-K+-ATPase. Am J Physiol. 1999 Sep;277(3 Pt 1):C361-72. {PubMed Link}

Rong Q, Utevskaya O, Ramilo M, Chow DC, Forte JG.  Nucleotide metabolism by gastric glands and H+-K+-ATPase-enriched membranes. Am J Physiol. 1998 Jan;274(1 Pt 1):G103-10. {PubMed Link}

Forte JG, Ly B, Rong Q, Ogihara S, Ramilo M, Agnew B, Yao X.  State of actin in gastric parietal cells. Am J Physiol. 1998 Jan;274(1 Pt 1):C97-104. {PubMed Link}

Peng XR, Yao X, Chow DC, Forte JG, Bennett MK.  Association of syntaxin 3 and vesicle-associated membrane protein (VAMP) with H+/K+-ATPase-containing tubulovesicles in gastric parietal cells. Mol Biol Cell. 1997 Mar;8(3):399-407. {PubMed Link}

Yao X, Cheng L, Forte JG.  Biochemical characterization of ezrin-actin interaction. J Biol Chem. 1996 Mar 22;271(12):7224-9. {PubMed Link}

Tyagarajan K, Townsend RR, Forte JG.  The Beta-subunit of the rabbit H,K-ATPase: a glycoprotein with all terminal lactosamine units capped with alpha-linked galactose residues. Biochemistry. 1996 Mar 12;35(10):3238-46. {PubMed Link}

Forte JG, Yao X.  The membrane-recruitment-and-recycling hypothesis of gastric HCl secretion. Trends Cell Biol. 1996 Feb;6(2):45-8. {PubMed Link}

Crothers JM Jr, Chow DC, Scalley ML, Forte JG.  In vivo trafficking of nascent H+-K+-ATPase in rabbit parietal cells. Am J Physiol. 1995 Dec;269(6 Pt 1):G883-91. {PubMed Link}

Tyagarajan K, Chow DC, Smolka A, Forte JG.  Structural interactions between alpha- and beta-subunits of the gastric H,K-ATPase. Biochim Biophys Acta. 1995 May 24;1236(1):105-13. {PubMed Link}

Thibodeau A, Kuo RC, Crothers JM Jr, Yao X, Owicki JC, Forte JG.  Direct measurement of extracellular proton flux from isolated gastric glands.  Am J Physiol Cell Physiol. 1994 Nov;267(5 pt 1):C1473-82. {PubMed Link}

Karam SM, Forte JG.  Inhibiting gastric H+-K+-ATPase activity by omeprazole promotes degeneration and production of parietal cells. Am J Physiol. 1994 Apr;266(4 Pt 1):G745-58. {PubMed Link}

Chow DC, Forte JG.  Characterization of the beta-subunit of the H+-K+-ATPase using an inhibitory monoclonal antibody. Am J Physiol. 1993 Dec;265(6 Pt 1):C1562-70. {PubMed Link}

Crothers JM Jr, Chow DC, Forte JG.  Omeprazole decreases H+-K+-ATPase protein and increases permeability of oxyntic secretory membranes in rabbits. Am J Physiol. 1993 Aug;265(2 Pt 1):G231-41. {PubMed Link}

Chow DC, Browning CM, Forte JG.  Gastric H+-K+-ATPase activity is inhibited by reduction of disulfide bonds in beta-subunit. Am J Physiol. 1992 Jul;263(1 Pt 1):C39-46. {PubMed Link}

Hanzel D, Reggio H, Bretscher A, Forte JG, Mangeat P.  The secretion-stimulated 80K phosphoprotein of parietal cells is ezrin, and has properties of a membrane cytoskeletal linker in the induced apical microvilli. EMBO J. 1991 Sep;10(9):2363-73. {PubMed Link} (Erratum in: EMBO J 1991 Dec;10(12):3978-81)

Crothers JM Jr, Reenstra WW, Forte JG.  Ontogeny of gastric H+-K+-ATPase in suckling rabbits. Am J Physiol. 1990 Dec;259(6 Pt 1):G913-21. {PubMed Link}

Canfield VA, Okamoto CT, Chow D, Dorfman J, Gros P, Forte JG, Levenson R.  Cloning of the H,K-ATPase beta subunit. Tissue-specific expression, chromosomal assignment, and relationship to Na,K-ATPase beta subunits. J Biol Chem. 1990 Nov 15;265(32):19878-84. {PubMed Link}

Reenstra WW, Forte JG.  Characterization of K+ and Cl- conductances in apical membrane vesicles from stimulated rabbit oxyntic cells. Am J Physiol. 1990 Nov;259(5 Pt 1):G850-8. {PubMed Link}

Okamoto CT, Karpilow JM, Smolka A, Forte JG.  Isolation and characterization of gastric microsomal glycoproteins. Evidence for a glycosylated beta-subunit of the H+/K+-ATPase. Biochim Biophys Acta. 1990 Mar 1;1037(3):360-72. {PubMed Link}

Forte JG, Hanzel DK, Urushidani T, Wolosin JM.  Pumps and pathways for gastric HCl secretion. Ann N Y Acad Sci. 1989;574:145-58. {PubMed Link}

Reenstra WW, Bettencourt JD, Forte JG.  Kinetic studies of the gastric H,K-ATPase. Evidence for simultaneous binding of ATP and inorganic phosphate. J Biol Chem. 1988 Dec 25;263(36):19618-25. {PubMed Link}

Urushidani T, Hanzel DK, Forte JG.  Protein phosphorylation associated with stimulation of rabbit gastric glands. Biochim Biophys Acta. 1987 Sep 14;930(2):209-19. {PubMed Link}

Hirst BH, Forte JG.  Redistribution and characterization of H++K+-ATPase membranes from resting and stimulated gastric parietal cells. Biochem J. 1985 Nov 1;231(3):641-9. {PubMed Link}

Wolosin JM, Forte JG.  Stimulation of oxyntic cell triggers K+ and Cl- conductances in apical H+-K+-ATPase membrane. Am J Physiol. 1984 May;246(5 Pt 1):C537-45. {PubMed Link}

Wolosin JM, Okamoto C, Forte TM, Forte JG.  Actin and associated proteins in gastric epithelial cells. Biochim Biophys Acta. 1983 Dec 13;761(2):171-82. {PubMed Link}

Wolosin JM, Forte JG.  Kinetic properties of the KCl transport at the secreting apical membrane of the oxyntic cell. J Membr Biol. 1983;71(3):195-207.

Forte JG, Forte TM, Black JA, Okamoto C, Wolosin JM.  Correlation of parietal cell structure and function. J Clin Gastroenterol. 1983;5 Suppl 1:17-27. Review. {PubMed Link}

Forte JG, Black JA, Forte TM, Machen TE, Wolosin JM.  Ultrastructural changes related to functional activity in gastric oxyntic cells. Am J Physiol. 1981 Nov;241(5):G349-58. {PubMed Link}

Black JA, Forte TM, Forte JG.  Inhibition of HCl secretion and the effects on ultrastructure and electrical resistance in isolated piglet gastric mucosa. Gastroenterology. 1981 Sep;81(3):509-19. {PubMed Link}

Wolosin JM, Forte JG.  Changes in the membrane environment of the K++H+-ATPase following stimulation of the gastric oxyntic cell. J Biol Chem. 1981 Apr 10;256(7):3149-52. {PubMed Link}

Reenstra WW, Forte JG.  H+/ATP stoichiometry for the gastric K++H+-ATPase. J Membr Biol. 1981;61(1):55-60. {PubMed Link}

Wolosin JM, Forte JG.  Isolation of the secreting oxyntic cell apical membrane — identification of an electroneutral KCl symport. Prog Clin Biol Res. 1981;73:189-204.

Lee HC, Forte JG.  A novel method for measurement of intravesicular pH using fluorescent probes. Biochim Biophys Acta. 1980 Sep 2;601(1):152-66. {PubMed Link}

Black JA, Forte TM, Forte JG.  Structure of oxyntic cell membranes during conditions of rest and secretion of HCl as revealed by freeze-fracture. Anat Rec. 1980 Feb;196(2):163-72. {PubMed Link}

Lee HC, Breitbart H, Forte JG.  The functional role of K+-ATPase in proton transport by gastric microsomal vesicles. Ann N Y Acad Sci. 1980;341:297-311.

Lee HC, Breitbart H, Berman M, Forte JG.  Potassium-stimulated ATPase activity and hydrogen transport in gastric microsomal vesicles. Biochim Biophys Acta. 1979 May 3;553(1):107-31. {PubMed Link}

Lee HC, Forte JG.  A study of H+ transport in gastric microsomal vesicles using fluorescent probes. Biochim Biophys Acta. 1978 Apr 4;508(2):339-56. {PubMed Link}

Forte TM, Machen TE, Forte JG.  Ultrastructural changes in oxyntic cells associated with secretory function: a membrane-recycling hypothesis. Gastroenterology. 1977 Oct;73(4 Pt 2):941-55. {Link}

Forte JG, Lee HC.  Gastric adenosine triphosphatases: a review of their possible role in HCl secretion. Gastroenterology. 1977 Oct;73(4 Pt 2):921-6. Review. {PubMed Link}

Ray TK, Forte JG.  Studies on the phosphorylated intermediates of a K+-stimulated ATPase from rabbit gastric mucosa. Biochim Biophys Acta. 1976 Sep 7;443(3):451-67. {PubMed Link}

Forte TM, Machen TE, Forte JG.  Ultrastructural and physiological changes in piglet oxyntic cells during histamine stimulation and metabolic inhibition. Gastroenterology. 1975 Dec;69(6):1208-22. {PubMed Link}

Forte JG, Ganser A, Beesley R, Forte TM.  Unique enzymes of purified microsomes from pig fundic mucosa. K+-stimulated adenosine triphosphatase and K+-stimulated pNPPase. Gastroenterology. 1975 Jul;69(1):175-89. {PubMed Link}

Forte JG, Forte TM, Machen TE.  Histamine-stimulated hydrogen ion secretion by in vitro piglet gastric mucosa. J Physiol. 1975 Jan;244(1):15-31. {PubMed Link}

Forte JG, Ganser AL, Tanisawa AS.  The K+-stimulated ATPase system of microsomal membranes from gastric oxyntic cells. Ann N Y Acad Sci. 1974;242(0):255-67.

Ganser AL, Forte JG.  Ionophoretic stimulation of K+-ATPase of oxyntic cell microsomes. Biochem Biophys Res Commun. 1973 Sep 18;54(2):690-6.

Ganser AL, Forte JG.  K+-stimulated ATPase in purified microsomes of bullfrog oxyntic cells. Biochim Biophys Acta. 1973 Apr 25;307(1):169-80.

Forte GM, Forte JG, Machen TE.  Active ion transport by isolated piglet gastric mucosa. J Physiol. 1972 Oct;226(2):31P-32P.

Forte T, Forte JG.  A freeze-fracture study of bullfrog gastric oxyntic cells. J Ultrastruct Res. 1971 Nov;37(3):322-34.

Tanisawa AS, Forte JG.  Phosphorylated-intermediate of microsomal ATPase from rabbit gastric mucosa. Arch Biochem Biophys. 1971 Nov;147(1):165-75.

Limlomwongse L, Forte JG.  Developmental changes in ATPase and K+-stimulated phosphatase of tadpole gastric microsomes. Am J Physiol. 1970 Dec;219(6):1717-22.

Forte GM, Limlomwongse L, Forte JG.  The development of intracellular membranes concomitant with the appearance of HCl secretion in oxyntic cells of the metamorphosing bullfrog tadpole. J Cell Sci. 1969 May;4(3):709-27. {PubMed Link}

Forte JG, Forte GM, Saltman P.  K+-stimulated phosphatase of microsomes from gastric mucosa. J Cell Physiol. 1967 Jun;69(3):293-304. {Link}

 

Updated Dec 3, 2012