Ryo Higuchi-Sanabria

Post-doctoral fellow

Research

My experience is considerably different from the typical researcher – I come from a background in restaurant and retail management, having worked as an assistant store manager, general store manager, and district manager in well-known companies including Burger King and Starbucks, as well as independent restaurants like Menchanko-Tei. These seemingly disconnected employments have given me the complex ability to multi-task and maximize my time, which have been instrumental in my undergraduate and graduate studies.

While juggling multiple responsibilities in my numerous jobs, I completed my BA and MA degree in Biotechnology at Hunter College, working with Dr. Paul Feinstein in characterization of odorant receptors in mouse olfactory neurons. Subsequently, I completed my PhD at Columbia University with Dr. Liza Pon working on identification of novel actin-mitochondrial interactions in regulation of cellular health and lifespan in the yeast model organism, S. cerevisiae. Throughout my research career, I also invested my time in developing my pedagogy, working as a teaching assistant and adjunct professor at several high schools, Kaplan, Hunter College, Columbia University, and College of Mount Saint Vincent.

In the Dillin lab, I plan to characterize actin cytoskeletal decline during advanced aging and to identify key components dedicated to protect cytoskeletal integrity during stress and aging.

Publications

Higuchi-Sanabria R, Vevea JD, Charalel JC, Sapar ML, Pon LA (2016). The transcriptional repressor Sum1p counteracts Sir2p in regulation of the actin cytoskeleton, mitochondrial quality control, and replicative lifespan in Saccharomyces Cerevisiae. Microbial Cell. In press.

Srivastava P, Alessi Wolken DM, Garcia-Rodriguez LJ, Higuchi-Sanabria R, Pon LA (2016). Organelle inheritance in yeast and other fungi. The Mycota. In press.

Higuchi-Sanabria R, Charalel JK, Viana M, Garcia EJ, Koenigsberg A, Swayne  TC,  Vevea JD, Boldogh I, Rafelski S, Pon LA (2016). Mitochondrial anchorage and fusion contribute to mitochondrial inheritance and quality control in the budding yeast, Saccharomyces cerevisiae. MBoC. PMID:26764088.

Higuchi-Sanabria R, Garcia EJ, Munteanu EL, Tomoiaga D, Feinstein P, Pon LA (2016). Characterization of fluorescent proteins for three- and four-color live-cell imaging in S. cerevisiae. PLoS One. PMID: 26727004.

Higuchi-Sanabria R, Swayne TC, Pon LA. Live-cell imaging of mitochondria and the actin cytoskeleton in budding yeast (2015). Methods Mol Biol. PMID: 26498779.

Higuchi-Sanabria R, Swayne TC, Boldogh IR, Pon LA (2015). Imaging of the actin cytoskeleton and mitochondria in fixed budding yeast cells. Methods Mol Biol.PMID: 26498779.

Higuchi-Sanabria R, Pernice WM, Vevea JD, Alessi Wolken DM, Boldogh IR, and Pon LA (2014). Damage sequestration and organelle rejuvenation promote cellular fitness and function. FEMS Yeast. 14 (8), 1133-1146. PMCID: PMC4270926.

Higuchi R, Vevea JD, Swayne TC, Chojnowski R, Hill V, Boldogh IR, and Pon LA (2013). Actin dynamics affects mitochondrial quality control and aging in budding yeast. Curr. Biol. 23, 2417-2422. PMCID: PMC3932488.