Erica Moehle, Ph.D.

Post-doctoral Fellow

 

Research

Between college and graduate school, I worked at Sangamo BioSciences, Inc. and helped develop what is now known as genome editing. I used zinc finger nucleases to determine conditions for integrating up to 8kb of DNA at an endogenous locus. In addition, I helped establish AAVS1 for use as a safe-harbor locus in the human genome.  For my PhD, I worked in Christine Guthrie’s lab at UCSF studying how pre-mRNA splicing is regulated in budding yeast. Budding yeast was thought to have relatively simple splicing regulation, as most intron-containing genes have only a single intron, but it was then discovered that most splicing occurs co-transcriptionally. I found that the efficiency of the splicing reaction is dependent on the chromatin structure of the DNA template and on how quickly the pre-mRNA is transcribed.  In the Dillin lab, I am studying a remarkable pathway called the mitochondrial unfolded protein response, which allows communication between mitochondria and the nucleus. I am interested in mitochondrial function and dysfunction in healthy human cells and in disease contexts.

 

Publications

Moehle EA, Braberg H, Krogan NJ, Guthrie C. 2014. Adventures in time and space: Splicing efficiency and RNA polymerase II elongation rate. RNA Biology 11:313–319.

Hérissant L*, Moehle EA*, Bertaccini D, Van Dorsselaer A, Schaeffer-Reiss C, Guthrie C, Dargemont C. 2014. H2B Ubiquitylation Modulates Spliceosome Assembly and Function in Budding Yeast. Biol. Cell 10.1111–boc.201400003.

Braberg H, Moehle EA, Shales M, Guthrie C, Krogan NJ. 2014. Genetic interaction analysis of point mutations enables interrogation of gene function at a residue-level resolution. Bioessays 36:706–713.

Braberg H, Jin H*, Moehle EA*, Chan YA, Wang S, Shales M, Benschop JJ, Morris JH, Qiu C, Hu F, Tang LK, Fraser JS, Holstege FCP, Hieter P, Guthrie C, Kaplan CD, Krogan NJ. 2013. From Structure to Systems: High-Resolution, Quantitative Genetic Analysis of RNA Polymerase II. Cell 154:775–788.

Moehle EA, Ryan CJ, Krogan NJ, Kress TL, Guthrie C. 2012. The Yeast SR-Like Protein Npl3 Links Chromatin Modification to mRNA Processing. PLoS Genet 8:e1003101.

DeKelver RC, Choi VM, Moehle EA, Paschon DE, Hockemeyer D, Meijsing SH, Sancak Y, Cui X, Steine EJ, Miller JC, Tam P, Bartsevich VV, Meng X, Rupniewski I, Gopalan SM, Sun HC, Pitz KJ, Rock JM, Zhang L, Davis GD, Rebar EJ, Cheeseman IM, Yamamoto KR, Sabatini DM, Jaenisch R, Gregory PD, Urnov FD. 2010. Functional genomics, proteomics, and regulatory DNA analysis in isogenic settings using zinc finger nuclease-driven transgenesis into a safe harbor locus in the human genome. Genome Res. 20:1133–1142.

Orlando SJ, Santiago Y, DeKelver RC, Freyvert Y, Boydston EA, Moehle EA, Choi VM, Gopalan SM, Lou JF, Li J, Miller JC, Holmes MC, Gregory PD, Urnov FD, Cost GJ. 2010. Zinc-finger nuclease-driven targeted integration into mammalian genomes using donors with limited chromosomal homology. Nucleic Acids Res. 38:e152.

Shukla VK, Doyon Y, Miller JC, DeKelver RC, Moehle EA, Worden SE, Mitchell JC, Arnold NL, Gopalan S, Meng X, Choi VM, Rock JM, Wu Y-Y, Katibah GE, Zhifang G, McCaskill D, Simpson MA, Blakeslee B, Greenwalt SA, Butler HJ, Hinkley SJ, Zhang L, Rebar EJ, Gregory PD, Urnov FD. 2009. Precise genome modification in the crop species Zea mays using zinc-finger nucleases. Nature 459:437–441.

Cai CQ, Doyon Y, Ainley WM, Miller JC, DeKelver RC, Moehle EA, Rock JM, Lee Y-L, Garrison R, Schulenberg L, Blue R, Worden A, Baker L, Faraji F, Zhang L, Holmes MC, Rebar EJ, Collingwood TN, Rubin-Wilson B, Gregory PD, Urnov FD, Petolino JF. 2008. Targeted transgene integration in plant cells using designed zinc finger nucleases. Plant Mol. Biol. 69:699–709.

Moehle EA*, Rock JM*, Lee Y-L, Jouvenot Y, DeKelver RC, Gregory PD, Urnov FD, Holmes MC. 2007. Targeted gene addition into a specified location in the human genome using designed zinc finger nucleases. Proc. Natl. Acad. Sci. 104:3055–3060.