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WEEK 1: Transport through the
nuclear pore complex (L1/2)
|
1
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Moore, M. S., and Blobel,
G. (1993). The GTP-binding
protein Ran/TC4 is required for protein import into the nucleus. Nature
365, 661-3. |
|
2
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Moore, M. S., and Blobel, G. (1992). The
two steps
of nuclear import, targeting to the nuclear envelope and translocation
through the nuclear pore, require different cytosolic factors. Cell 69,
939-50. |
3
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Isolation
of a protein
that is essential for the first step of nuclear protein import.
(Cell
79, 767-778). |
|
4
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The yeast nuclear
pore: composition and architecture (JCB 148: 635) |
|
5
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The NPC may
operate by hydrophobic exclusion (EMBO J, 21: 2664) |
|
6
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Transport
into and out of the Nucleus (Review) |
|
7
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Regulating access to the genome:
nucleocytoplasmic transport during the cell cycle (Review)
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WEEK 2: RNA transport, maturation
& localization (L1/2)
|
|
1
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RNA
export (Review) |
2
|
RNA
transport and local
control of translation (Review) |
3
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Gene
gating? (Casolari et al, Cell 2004 117:427)
|
4
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Specificity
and regulation of mRNA decay (PNAS 2002 99:5860)
|
| 5 |
mRNA
turnover in cytoplasmic p bodies (Science 2003 300:805)
|
6
|
Definition
of nonsense
codons for NMD (EMBO J 1998 17:3484) |
7**
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FG-repeats
can form a hydrogel (Science 5800:815-817) |
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WEEK 3: Translocation into the ER
and vesicular transport (L1,
L2)
|
1
|
Characterization of the secretory
pathway by Novick, Field and Schekman (classic paper) |
2
|
In vitro assay to monitor Golgi
transport by Rothman and colleagues (classic paper) |
3
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Signal sequence
recognition and protein targeting. (Review) |
4
|
Structure of the protein
conducting channel. |
5
|
Vesicle
budding and fusion. (Review) |
6
|
Structure
of the COPII coat. |
7**
|
BiP acts as a molecular ratchet
during posttranslational transport across the ER membrane. |
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WEEK 4: Secretion & cell
polarity (LI, L2)
|
1
|
The
Exocyst (Review)
|
2
|
Analysis
of exocytosis in epithelial cells (Nat Cell Biol 2003 2:126-36)
|
3
|
Polarized
targeting of the LDL receptor (Cell 2001 105:575-85)
|
| 4 |
The Golgi matrix during
mitosis.
|
5
|
Procollagen
transport:
evidence for cisternal maturation. |
6**
|
Complexin
and synaptotagmin regulate synaptic exocytosis (Science 2006
313:676 - 680)
|
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WEEK 5: Endocytosis, organelle
inheritance and transport into mitochondria (L1/L2)
|
1
|
Distribution of Golgi membranes
during mitosis
|
2
|
The
Golgi matrix during
mitosis. |
3
|
Procollagen
transport:
evidence for cisternal maturation. |
4
|
Protein
import into mitochondria (Review)
|
5**
|
In
vitro assay to monitor mitochondrial fusion (Science 2004
305:1747-1752)
|
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*** Key for Exam ***
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