34
Blastocyst
Injection
Injection
¥Start
with correctly targeted ES cell
¥Hold
blastocyst with suction pipette
¥Inject
ES cells into blastocyst
¥Injected ES cells
become part of host blastocyst¥Implant
embryo into foster mother
¥Embryo
develops into chimeric mouse
¥Breed
to achieve germline transmission