The objective of this course is to provide an introduction to
experimental design and basic techniques commonly used in immunology
research laboratories. The course is intended for upper-division
undergraduate students majoring in immunology and those majoring in
other biological sciences who plan to use immunological techniques in
independent-study projects in the research laboratories or in
employment.
The module on immunochemistry and cell culture provides
experience in preparation of cell suspensions from lymphoid organs of
mice, an assay for detection of activated T cells, production of
monoclonal antibodies, enzyme-linked immunosorbent assay,
immunoprecipitation, SDS-PAGE, western blot and staining of cells for
flow cytometry.
The molecular module provides experience in isolation
of DNA, Southern blotting, restriction mapping, subcloning, and DNA
sequencing. Emphasis will be placed on experimental design and the
interpretation of data.
A working knowledge of basic immunology is
required; either previous completion of an upper-division course in
immunology or concurrent enrollment in MCB 150 is sufficient. The
laboratory work will include making and working with cell suspensions
from spleens and thymuses of mice that have been euthanized with
CO2 by the staff immediately before the laboratory period.
It is not possible to immunize cell lines; cells from normal or
immunized mice must be used for experiments that involve production
of antibody responses in tissue culture. Therefore, this course is
not suitable for students who object to the use of animals in
teaching and/or research.