Two new studies should give scientists who use CRISPR-Cas9 for genome engineering greater confidence that they won’t inadvertently edit the wrong DNA. The two new reports from the Doudna and Tjian Labs show in much greater detail how the Cas9 protein searches through billions of base pairs in a cell to find the right DNA sequence, and how Cas9 determines whether to bind, or bind and cut, thereby initiating gene editing.
Also, in a new video, Professor of Biochemistry, Biophysics and Structural Biology Jennifer Doudna explains how CRISPR-Cas9 works. Watch the video!