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Older Papers from the Schekman Lab (1990-2000)


Todorow Z, Spang A, Carmack E, Yates J, Schekman R.
Active recycling of yeast golgi mannosyltransferase complexes through the endoplasmic reticulum. PNAS 97, 13643-8 (2000).

Shimoni Y, Kurihara T, Ravazzola M, Amherdt M, Orci L, Schekman R.
Lst1p and sec24p cooperate in sorting of the plasma membrane ATPase into COPII vesicles in saccharomyces cerevisiae. J Cell Biol 151, 973-84 (2000).

Springer, S., Chen, E., Duden, R., Marzioch, M., Rowley, A., Hamamoto, S., Merchant, S., and Schekman, R
The p24 proteins are not essential for vesicular transport in Saccharomyces cerevisiae. PNAS 97, 4034-4039 (2000).

Matsuoka, K., and Schekman, R
The use of liposomes to study COPII and COPI coated vesicle formation and membrane sorting. Meth. Enzymol. 20, 417-428 (2000).

Kurihara, T., Hamamoto, S., Gimeno, R. E., Kaiser, C. A., Schekman, R., and Yoshihisa, T.
Sec24p and Iss1p function interchangeably in transport vesicle formation from the endoplasmic reticulum in Saccharomyces cerevisiae. Mol. Biol. Cell 11, 983-998 (2000).

Lau, W.-T. W., Howson, R. W., Malkus, P., Schekman, R., and O'Shea, E. K.
Pho86p, an ER resident protein in Saccharomyces cerevisiae, is required for ER exit of the high affinity phosphate transporter Pho84p. PNAS 97, 1107-1112 (2000).


Zhou, M. and Schekman, R.
The engagement of Sec61p in the ER dislocation process. Mol Cell 4, 925-934 (1999).

Pilon, M and Schekman, R.
Protein translocation : How Hsp70 pulls it off. Cell 97, 679-682 (1999).

Sebastian Springer, Anne Spang and Randy Schekman
A Primer on Vesicle Budding. Cell 97, 145 - 148. (1999).

Johannes Hermann, Per Malkus, and Randy Schekman
Out of the ER - outfitters, escorts and guides. Trends in Cell Biology 9, 5-7. (1999).


Anne Spang and Randy Schekman
Reconstitution of Retrograde Transport from the Golgi to the ER In Vitro. JCB 143, pp589-599. (1998).

Marinus Pilon, Karin Romisch, Dong Quach and Randy Schekman
Sec61p Serves Multiple Roles in Secretory Precursor Binding and Translocation into the Endoplasmic Reticulum Membrane. Molecular Biology of the Cell 9 p. 3455 - 3473 (1998).

Ken Matsuoka, Yasujiro Morimitsu, Koji Uchida, and Randy Schekman
Coat Assembly Directs v-SNARE Concentration into Synthetic COPII Vesicles. Molecular Cell 2 pp703-708 (1998).

Anne Spang, Ken Matsuoka, Susan Hamamoto, Randy Schekman, and Lelio Orci
Coatomer, Arf1p, and nucleotide are required to bud coat protein complexI-coated vesicles from large synthetic liposomes. PNAS 95pp11199-11204 (1998).

Meta J. Kuehn, Johannes Herrmann and Randy Schekman
COPII-cargo interactions direct protein sorting into ER-derived transport vesicles. Nature. 391: 187 - 190 (Jan 8, 1998).

Sebastian Springer and Randy Schekman (1998)
Nucleation of COPII Vesicular Coat Complex by ER to Golgi v-SNAREs. : Science 281p. 698-700.

Ziman M, Chuang JS, Tsung M, Hamamoto S, Schekman R
Chs6p-dependent anterograde transport of Chs3p from the chitosome to the plasma membrane in Saccharomyces cerevisiae. Mol Biol Cell 1998 Jun;9(6):1565-1576.

Matsuoka K, Orci L, Amherdt M, Bednarek SY, Hamamoto S, Schekman R, Yeung T
COPII-coated vesicle formation reconstituted with purified coat proteins and chemically defined liposomes. Cell 1998 Apr 17;93(2):263-275.

Duden R, Kajikawa L, Wuestehube L, Schekman R
epsilon-COP is a structural component of coatomer that functions to stabilize alpha-COP. EMBO J 1998 Feb 16;17(4):985-995.

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Marinus Pilon, Randy Schekman, and Karin Romisch
Sec61p-dependent export of misfolded secretory proteins from the ER to the cytosol. EMBO J. 16: 4540-4548 (1997).

Schekman, R., and Mellman, I.
Does COPI Go Both Ways? Cell 90:197-200 (1997).

Meta Kuehn and Randy Schekman
COPII and secretory cargo capture into transport vesicles. Curr. Op. in Cell Bio. 9:477-483 (1997).

Ann K. Corsi and Randy Schekman
The Lumenal Domain of Sec63p Stimulates the ATPase Activity of BiP and Mediates BiP Recruitment to the Translocon in S. cerevisiae. JCB, Vol. 137, 1483-1493 (1997).

Nina Salama, John Chuang, and Randy Schekman.
SEC31 encodes an essential component of the COPII coat required for transport vesicle budding from the endoplasmic reticulum. Mol. Bio. Cell 8:205-217 (1997).

Joseph Campbell and Randy Schekman
Selective packaging of cargo molecules into endoplasmic reticulum-derived COPII vesicles. PNAS 94:837-842 (1997).


S. K. Lyman and Randy Schekman
Polypeptide translocation machinery of the yeast endoplasmic reticulum. Experientia 52:1042-1049 (1996).

Sebastian Y. Bednarek, Lelio Orci and Randy Schekman
Traffic COPs and the formation of vesicle coats. Trends in Cell Biology, Vol. 6, Dec., 1996.

Randy Schekman
Polypeptide Translocation: A Pretty Picture Is Worth a Thousand Words. Cell, Vol. 87, 593-595.

Michael Ziman, John S. Chuang and Randy Schekman
Chs1p and Chs3p, Two Proteins Involved in Chitin Synthesis, Populate a Compartment of the Saccharomyces cerevisiae Endocytic Pathway. Mol. Bio. of the Cell, Vol. 7, 1909-1919 .

Ann K. Corsi and Randy Schekman
Mechanism of Polypeptide Translocation into the Endplasmic Reticulum. J. Bio. Chem., Vol. 271, 30299-30302.

John S. Chuang and Randy Schekman
Differential Trafficking and Timed Localization of Two Chitin Synthase Proteins, Chs2p and Chs3p. J. of Cell Biology, Vol. 135, 597-610.

Randy Schekman
Regulation of membrane traffic in the secretory pathway. Harvey Lectures, Academic Press, (1996).

Meta J. Kuehn, Randy Schekman and Per O. Ljungdahl
Amino Acid Permeases Require COPII Components and ER Resident Membrane Protein Shr3p for Packaging into Transport Vesicles In Vitro. J. Cell Bio., Vol. 135, 585-595.

Tamara Doering and Randy Schekman
GPI-anchor attachment is required for Gas1p transport from the endoplasmic reticulum in COPII vesicles. EMBO Journal, Vol. 15, 182-191 (1996).

Wuestehube, L. J., Duden, R., Eun, A., Hamamoto, S., Korn, P., Ram, R., and Schekman, R.
New mutants of Saccharomyces cerevisiae affected in the transport of proteins from the endoplasmic reticulum to the Golgi complex. Genetics, Vol. 142, 393-406 (1996).

Sebastian Y. Bednarek, Mariella Ravazzola, Midori Hosobuchi, Mylene Amherdt, Alain Perrelet, Randy Schekman, and Lelio Orci.
COPI- and COPII-coated vesicles bud directly from the endoplasmic reticulum in yeast. Cell Vol 83, 1183-1196.

Lupashin, V. V., Hamamoto, S., and Schekman, R. (1996)
Biochemical requirements for the targeting and fusion of ER-derived transport vesicles with purified yeast Golgi membranes. Journal of Cell Biology, Vol. 132, 277-289.

Randy Schekman and Lelio Orci
Coat Proteins and Vesicle Budding. Science, Vol. 271, 1526-1533 (March 15,1996).

Schekman, R., Barlowe, C., Bednarek, S., Campbell, J., Doering, T., Duden, R., Kuehn, M., Rexach, M., Yeung, T., and Orci, L. (1996)
Coat proteins and selective protein packaging into transport vesicles. In "The Dynamics of Protein Trafficking and Stability" (B.Stillman, ed), Symposium 60, Cold Spring harbor Press, Cold Spring harbor, NY.


Susie Lyman and Randy Schekman
Interaction between BiP and Sec63p is required for the completion of protein translocation into the ER of S. cerevisiae. Journal of Cell biology, Vol 131, 1163-1171 (1995).

Brodsky JL; Goeckeler J; Schekman R.
BiP and Sec63p are required for both co- and posttranslational protein translocation into the yeast endoplasmic reticulum. Proceedings of the National Academy of Sciences of the United States of America, 1995 Oct 10, 92(21):9643-6.

Latterich M; Frohlich KU; Schekman R.
Membrane fusion and the cell cycle: Cdc48p participates in the fusion of ER membranes. Cell, 1995 Sep 22, 82(6):885-93.

Salama NR; Schekman RW.
The role of coat proteins in the biosynthesis of secretory proteins. Current Opinion in Cell Biology, 1995 Aug, 7(4):536-43.

Barlowe, C. and Schekman, R. (1995)
Expression, purification, and assay of Sec12p: A Sar1p-specific GDP dissociation stimulator. Methods in Enzymology, vol 257, Part C (Balch, W. E., Der, C. J., Hall, A., eds.), 97-106

Thomas Yeung, Tohru Yoshihisa and Randy Schekman
Purification of Sec23p/Sec24p complex. Methods in Enzymology, vol 257, Part C (Balch, W. E., Der, C. J., Hall, A., eds.), 145-151.

Yeung, T., Barlowe, C., and Schekman, R.
Uncoupled packaging of targeting and cargo molecules during transport vesicle budding from the endoplasmic reticulum. Journal of Biological Chemistry, Vol. 270, 30567-30570 (1995).

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Orci L; Perrelet A; Ravazzola M; Amherdt M; Rothman JE; Schekman R. Coatomer-rich endoplasmic reticulum. Proceedings of the National Academy of Sciences of the United States of America, 1994 Dec 6, 91(25):11924-8.

Esnault Y; Feldheim D; Blondel MO; Schekman R; Kepes F. SSS1 encodes a stabilizing component of the Sec61 subcomplex of the yeast protein translocation apparatus. Journal of Biological Chemistry, 1994 Nov 4, 269(44):27478-85.

Duden R; Hosobuchi M; Hamamoto S; Winey M; Byers B; Schekman R. Yeast beta- and beta'-coat proteins (COP). Two coatomer subunits essential for endoplasmic reticulum-to-Golgi protein traffic. Journal of Biological Chemistry, 1994 Sep 30, 269(39):24486-95.

Schekman R. Translocation gets a push. Cell, 1994 Sep 23, 78(6):911-3.

Rexach MF; Latterich M; Schekman RW. Characteristics of endoplasmic reticulum-derived transport vesicles. Journal of Cell Biology, 1994 Sep, 126(5):1133-48.

Kurihara LJ; Beh CT; Latterich M; Schekman R; Rose MD. Nuclear congression and membrane fusion: two distinct events in the yeast karyogamy pathway. Journal of Cell Biology, 1994 Aug, 126(4):911-23.

Feldheim D; Schekman R. Sec72p contributes to the selective recognition of signal peptides by the secretory polypeptide translocation complex. Journal of Cell Biology, 1994 Aug, 126(4):935-43.

Latterich M; Schekman R. The karyogamy gene KAR2 and novel proteins are required for ER-membrane fusion. Cell, 1994 Jul 15, 78(1):87-98.

Barlowe C; Orci L; Yeung T; Hosobuchi M; Hamamoto S; Salama N; Rexach MF; Ravazzola M; Amherdt M; Schekman R. COPII: a membrane coat formed by Sec proteins that drive vesicle budding from the endoplasmic reticulum. Cell, 1994 Jun 17, 77(6):895-907.


Orci L; Perrelet A; Ravazzola M; Wieland FT; Schekman R; Rothman JE. "BFA bodies": a subcompartment of the endoplasmic reticulum. Proceedings of the National Academy of Sciences of the United States of America, 1993 Dec 1, 90(23):11089-93.

Brodsky JL; Schekman R. A Sec63p-BiP complex from yeast is required for protein translocation in a reconstituted proteoliposome. Journal of Cell Biology, 1993 Dec, 123(6 Pt 1):1355-63.

Salama NR; Yeung T; Schekman RW. The Sec13p complex and reconstitution of vesicle budding from the ER with purified cytosolic proteins. Embo Journal, 1993 Nov, 12(11):4073-82.

Barlowe C; Schekman R. SEC12 encodes a guanine-nucleotide-exchange factor essential for transport vesicle budding from the ER. Nature, 1993 Sep 23, 365(6444):347-9.

Feldheim D; Yoshimura K; Admon A; Schekman R. Structural and functional characterization of Sec66p, a new subunit of the polypeptide translocation apparatus in the yeast endoplasmic reticulum. Molecular Biology of the Cell, 1993 Sep, 4(9):931-9.

Yoshihisa T; Barlowe C; Schekman R. Requirement for a GTPase-activating protein in vesicle budding from the endoplasmic reticulum. Science, 1993 Mar 5, 259(5100):1466-8.

Pryer NK; Salama NR; Schekman R; Kaiser CA. Cytosolic Sec13p complex is required for vesicle formation from the endoplasmic reticulum in vitro. Journal of Cell Biology, 1993 Feb, 120(4):865-75.

Barlowe C; d'Enfert C; Schekman R. Purification and characterization of SAR1p, a small GTP-binding protein required for transport vesicle formation from the endoplasmic reticulum. Journal of Biological Chemistry, 1993 Jan 15, 268(2):873-9.

Brodsky JL; Hamamoto S; Feldheim D; Schekman R. Reconstitution of protein translocation from solubilized yeast membranes reveals topologically distinct roles for BiP and cytosolic Hsc70. Journal of Cell Biology, 1993 Jan, 120(1):95-102.


Hosobuchi M; Kreis T; Schekman R. SEC21 is a gene required for ER to Golgi protein transport that encodes a subunit of a yeast coatomer. Nature, 1992 Dec 10, 360(6404):603-5.

Romisch K; Schekman R. Distinct processes mediate glycoprotein and glycopeptide export from the endoplasmic reticulum in Saccharomyces cerevisiae. Proceedings of the National Academy of Sciences of the United States of America, 1992 Aug 1, 89(15):7227-31.

Schekman R. Genetic and biochemical analysis of vesicular traffic in yeast. Current Opinion in Cell Biology, 1992 Aug, 4(4):587-92.

Sanders SL; Schekman R. Polypeptide translocation across the endoplasmic reticulum membrane. Journal of Biological Chemistry, 1992 Jul 15, 267(20):13791-4.

Feldheim D; Rothblatt J; Schekman R. Topology and functional domains of Sec63p, an endoplasmic reticulum membrane protein required for secretory protein translocation. Molecular and Cellular Biology, 1992 Jul, 12(7):3288-96.

Griff IC; Schekman R; Rothman JE; Kaiser CA. The yeast SEC17 gene product is functionally equivalent to mammalian alpha-SNAP protein. Journal of Biological Chemistry, 1992 Jun 15, 267(17):12106-15.

Hicke L; Yoshihisa T; Schekman R. Sec23p and a novel 105-kDa protein function as a multimeric complex to promote vesicle budding and protein transport from the endoplasmic reticulum. Molecular Biology of the Cell, 1992 Jun, 3(6):667-76.

Sanders SL; Whitfield KM; Vogel JP; Rose MD; Schekman RW. Sec61p and BiP directly facilitate polypeptide translocation into the ER. Cell, 1992 Apr 17, 69(2):353-65.

Rexach M; d'Enfert C; Wuestehube L; Schekman R. Genes and proteins required for vesicular transport from the endoplasmic reticulum. Antonie Van Leeuwenhoek, 1992 Feb, 61(2):87-92.

Stirling CJ; Rothblatt J; Hosobuchi M; Deshaies R; Schekman R. Protein translocation mutants defective in the insertion of integral membrane proteins into the endoplasmic reticulum. Molecular Biology of the Cell, 1992 Feb, 3(2):129-42.

Pryer NK; Wuestehube LJ; Schekman R. Vesicle-mediated protein sorting. Annual Review of Biochemistry, 1992, 61:471-516.

Wuestehube LJ; Schekman RW. Reconstitution of transport from endoplasmic reticulum to Golgi complex using endoplasmic reticulum-enriched membrane fraction from yeast. Methods in Enzymology, 1992, 219:124-36.

Rexach MF; Schekman RW. Use of sec mutants to define intermediates in protein transport from endoplasmic reticulum. Methods in Enzymology, 1992, 219:267-86.

Hicke L; Yoshihisa T; Schekman RW. Purification of yeast Sec23 protein by complementation of mutant cell lysates deficient in endoplasmic reticulum-to-Golgi transport. Methods in Enzymology, 1992, 219:338-52.


d'Enfert C; Barlowe C; Nishikawa S; Nakano A; Schekman R. Structural and functional dissection of a membrane glycoprotein required for vesicle budding from the endoplasmic reticulum. Molecular and Cellular Biology, 1991 Nov, 11(11):5727-34.

Orci L; Ravazzola M; Meda P; Holcomb C; Moore HP; Hicke L; Schekman R. Mammalian Sec23p homologue is restricted to the endoplasmic reticulum transitional cytoplasm. Proceedings of the National Academy of Sciences of the United States of America, 1991 Oct 1, 88(19):8611-5.

d'Enfert C; Wuestehube LJ; Lila T; Schekman R. Sec12p-dependent membrane binding of the small GTP-binding protein Sar1p promotes formation of transport vesicles from the ER. Journal of Cell Biology, 1991 Aug, 114(4):663-70.

Rexach MF; Schekman RW. Distinct biochemical requirements for the budding, targeting, and fusion of ER-derived transport vesicles. Journal of Cell Biology, 1991 Jul, 114(2):219-29.

Chiang HL; Schekman R. Regulated import and degradation of a cytosolic protein in the yeast vacuole. Nature, 1991 Mar 28, 350(6316):313-8.

Deshaies RJ; Sanders SL; Feldheim DA; Schekman R. Assembly of yeast Sec proteins involved in translocation into the endoplasmic reticulum into a membrane-bound multisubunit complex. Nature, 1991 Feb 28, 349(6312):806-8.

Franzusoff A; Redding K; Crosby J; Fuller RS; Schekman R. Localization of components involved in protein transport and processing through the yeast Golgi apparatus. Journal of Cell Biology, 1991 Jan, 112(1):27-37.

Franzusoff A; Rothblatt J; Schekman R. Analysis of polypeptide transit through yeast secretory pathway. Methods in Enzymology, 1991, 194:662-74.

Deshaies RJ; Schekman R. Structural and functional dissection of Sec62p, a membrane-bound component of the yeast endoplasmic reticulum protein import machinery. Molecular and Cellular Biology, 1990 Nov, 10(11):6024-35.


Silveira LA; Wong DH; Masiarz FR; Schekman R. Yeast clathrin has a distinctive light chain that is important for cell growth. Journal of Cell Biology, 1990 Oct, 111(4):1437-49.

Hicke L; Schekman R. Molecular machinery required for protein transport from the endoplasmic reticulum to the Golgi complex. Bioessays, 1990 Jun, 12(6):253-8.

Kaiser CA; Schekman R. Distinct sets of SEC genes govern transport vesicle formation and fusion early in the secretory pathway. Cell, 1990 May 18, 61(4):723-33.

Sengstag C; Stirling C; Schekman R; Rine J. Genetic and biochemical evaluation of eucaryotic membrane protein topology: multiple transmembrane domains of Saccharomyces cerevisiae 3-hydroxy-3-methylglutaryl coenzyme A reductase. Molecular and Cellular Biology, 1990 Feb, 10(2):672-80.

Baker D; Wuestehube L; Schekman R; Botstein D; Segev N. GTP-binding Ypt1 protein and Ca2+ function independently in a cell-free protein transport reaction. Proceedings of the National Academy of Sciences of the United States of America, 1990 Jan, 87(1):355-9.

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