| VECTOR
STOCKS: in -80 archive freezer |
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| NAME |
FROM |
DATE |
DILUTED IN |
USED FOR |
#TUBES |
CAME FROM |
MORE INFO |
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| VECTOR
STOCK BOX 1 |
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| C115
Rae1D |
TJ |
05/11/05 |
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1 |
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| gagpol |
TJ |
01/31/08 |
1.87ug/ul |
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1 |
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| MCMV-Thy1.1-OVA |
TJ |
08/31/07 |
0.33ug/ul |
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1 |
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| MCSV-CMV
Rae1E GFP |
TJ |
02/29/04 |
0.70ug/ul |
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1 |
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clone 1 |
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| MCSV-D
Rae1B-GMCSF |
TJ |
09/10/05 |
0.50ug/ul |
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1 |
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clone 7 |
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| MCV CMV
Rae1E |
TJ |
03/03/07 |
1.10ug/ul |
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1 |
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| MFG Rae1
B |
TJ |
01/31/08 |
1.10ug/ul |
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1 |
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| MFG
Vector |
TJ |
01/31/08 |
1.18ug/ul |
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1 |
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| MFG-H60 |
TJ |
01/31/08 |
1.22ug/ul |
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1 |
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| MFG-Rae1D |
TJ |
09/02/05 |
0.80ug/ul |
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1 |
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| MSCV 2.2 |
TJ |
10/06/04 |
1.30ug/ul |
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1 |
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| MSCV CMV
D GFP |
TJ |
03/03/04 |
0.90ug/ul |
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1 |
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| MSCV
Thy1.1 |
TJ |
08/31/07 |
1.25ug/ul |
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1 |
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| MSCV
Thy1.1 |
SML |
09/28/07 |
H2O |
miniprep DNA |
1 |
Sha Lab |
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| MSCV
Thy1.1 OVA |
SML |
09/28/07 |
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miniprep DNA |
1 |
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| MSCV2.2-Rae1E |
TJ |
01/31/08 |
1.00ug/ul |
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1 |
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| MSCV22-Rae1E |
TJ |
03/12/05 |
4.10ug/ul |
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1 |
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| MSCV-CMV-GFP |
TJ |
03/03/04 |
0.75ug/ul |
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1 |
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| MSCV-CMV-Rae1D
IRES GMCSF |
TJ |
07/06/05 |
1.30ug/ul |
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1 |
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| MSCV-CMV-Rae1E
IRES GMCSF |
TJ |
03/08/05 |
0.90ug/ul |
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1 |
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| MSCV-CMV-RaeD |
TJ |
05/18/05 |
1.10ug/ul |
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1 |
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| MSCVD
Rae1B GMCSF |
TJ |
09/10/05 |
0.55ug/ul |
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1 |
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clone 13 |
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| MSCV-D
Rae1D |
TJ |
07/25/05 |
0.84ug/ul |
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1 |
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| MSCV-H60 |
TJ |
07/13/06 |
0.72ug/ul |
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1 |
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clone 3 |
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| MSCV-H60 |
TJ |
07/13/06 |
0.57ug/ul |
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1 |
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clone 5 |
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| PACXIX-Rae1D |
TJ |
09/12/05 |
0.40ug/ul |
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1 |
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clone 20 |
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| pBluescript
OVA |
SML |
09/28/07 |
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to make MSCV-Thy1.1 OVA |
1 |
Shastri Lab |
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| pBs -
IRES |
TJ |
01/10/04 |
0.73ug/ul |
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1 |
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(meth-) |
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| pBs
IRES-GMCSF-Adaptor |
TJ |
05/21/05 |
0.97ug/ul |
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1 |
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| pBs-IRES |
TJ |
01/31/08 |
2.00ug/ul |
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1 |
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| pcDNA
OVA |
SML |
09/28/07 |
PBS |
to make MSCV-Thy1.1 OVA |
1 |
Shastri Lab |
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| pCMK
gagpd |
TJ/DS |
01/31/08 |
3.347ug/ul |
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1 |
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| Pellet
Paint Co-Precipitant |
TJ |
01/31/08 |
125ug/ul |
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1 |
Novagen |
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| pGEM-GMCSF |
TJ |
07/01/03 |
2.00ug/ul |
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1 |
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pGEM T Easy GMCSF |
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| pGEMT-FMCSF |
TJ |
01/10/04 |
0.60ug/ul |
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1 |
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(meth-) |
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| pHCMV-11
VSV |
TJ |
01/31/08 |
1.78ug/ul |
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1 |
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| pHSCV
H60 |
TJ |
01/31/08 |
1.10ug/ul |
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1 |
|
A1 |
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| PKS - |
SML |
09/28/07 |
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1 |
Invitrogen |
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| pMFG-GMCSF |
TJ |
01/31/08 |
1.00ug/ul |
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1 |
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| pMFG-Mike |
TJ |
01/31/08 |
0.5ug/ul |
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1 |
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| pMG-GMCSF
Ly+2 |
TJ |
01/31/08 |
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1 |
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| pMG-GMCSF
Ly+2 |
TJ |
01/31/08 |
0.90ug/ul |
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1 |
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| pMSCV -
MULT |
TJ |
08/08/04 |
1.00ug/ul |
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1 |
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clone 12 |
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| pMSCV
CMV GFP |
TJ |
01/31/08 |
1.10ug/ul |
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1 |
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| pMSCV D
MULT |
TJ |
08/08/04 |
1.00ug/ul |
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1 |
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clone 1 |
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| pMX GFP |
TJ |
01/31/08 |
0.50ug/ul |
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1 |
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| pQC-XIP-Rae1D |
TJ |
08/29/05 |
0.40ug/ul |
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1 |
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| PQC-XIX |
TJ |
08/31/05 |
0.40ug/ul |
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1 |
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| VpR |
FD |
11/15/08 |
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31 |
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| VpR |
FD |
11/16/08 |
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2 |
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| VECTOR
STOCK BOX 2 |
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| NAME |
FROM |
DATE |
DILUTED IN |
USED FOR |
#TUBES |
CAME FROM |
MORE INFO |
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| 1C181-BAC
DNA |
FD |
06/04 |
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1 |
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| HIV HAS+
VA- E- VpR |
FD |
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1 |
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| HIV HS+
VpR+ E- |
FD |
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1 |
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| NL43
VpR- |
FD |
03/03/08 |
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1 |
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| NL43
VpR+ |
FD |
03/03/08 |
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1 |
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| P161-GFP |
FD |
06/22/07 |
1 mg/ml |
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1 |
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| P161-GFP |
FD |
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1.5 ug/10ul TE |
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1 |
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| PCVSVg |
FD |
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0.5 mg/ml |
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1 |
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| PDR9 |
FD |
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1.3 ug/10ul TE |
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1 |
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| PEGFP-C1 |
FD |
11/05/05 |
1 ug/ml |
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1 |
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| PG 1 |
FD |
06/22/07 |
1 ug/ml |
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1 |
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| PHR-VpR |
FD |
06/22/07 |
1 mg/ml |
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1 |
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| PHR-VPRIRES |
FD |
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1.78 ug/10ul TE |
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1 |
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| PVSV-G |
FD |
02/10/05 |
1 ug/ml |
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1 |
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| PVSV-G |
FD |
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2.7 ug/10ul TE |
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1 |
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| VpR |
FD |
11/16/08 |
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22 |
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| VSVG |
FD |
03/03/08 |
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1 |
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| pSpax2 |
MW |
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1.6 |
transduction pFG12 |
1 |
Schlissel lab |
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| pFG12
HIF-1a 3mutations |
MW |
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1 |
inducible hif-1a |
1 |
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| pcDNA3
HIF-1a dPA |
MW |
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1.3 |
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1 |
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| pVEGF-Luc |
MW |
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1.54 |
HRE |
1 |
Winoto lab |
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| pGL3-PF1-ER3
#4 |
MW |
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0.0995 |
h60c promoter luciferase |
1 |
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| pGL3-PF1-ER3
#2 |
MW |
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0.744 |
h60c promoter luciferase |
1 |
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| pGL3-PF1-ER3
#3 |
MW |
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0.21 |
h60c promoter luciferase |
1 |
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| pcDNA3
HIF-1a 3mutation |
MW |
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1.16 |
inducible hif-1a |
1 |
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| pGL3-PF1-ER3
#5 |
MW |
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0.14 |
h60c promoter luciferase |
1 |
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| pFG12
HIF-2a dPA |
MW |
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1.56 |
inducible hif-2a |
1 |
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| pFG12-TRE-UbC-rtTA-Thy1.1 |
MW |
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2 |
dox inducible vector |
1 |
Schlissel lab |
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| pFG12
HIF-1a dPA |
MW |
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0.22 |
inducible hif-1a |
1 |
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| pcDNA3
HIF-2a dPA |
MW |
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0.23 |
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1 |
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| pGL3-PF1-ER5
#8 |
MW |
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0.119 |
h60c promoter luciferase |
1 |
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| pcDNA3-CD4 |
MW |
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2.1 |
may be human CD4, not sure |
1 |
Coscoy lab |
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| pGL3
control vector |
MW |
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0.6 |
firefly luciferase |
1 |
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| pGL3
basic vector |
MW |
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0.8 |
firefly luciferase |
1 |
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| pGL3
promoter vector |
MW |
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1.1 |
firefly luciferase |
1 |
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| pGL3-PF1-ER5
#2 |
MW |
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1.2 |
h60c promoter luciferase |
1 |
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| pGL3-PF1-ER5
Reverse |
MW |
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0.77 |
h60c promoter luciferase |
1 |
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| pEFRL |
MW |
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1.4 |
renilla luciferase |
1 |
Winoto lab |
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| pCI |
MW |
|
2 |
mammalian expression vector |
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Winoto lab |
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| pGL3
enhancer vector |
MW |
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0.12 |
firefly luciferase |
1 |
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| pGL3-PF1-ER5
#1 |
MW |
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h60c promoter luciferase |
1 |
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| pGL3-PF1-ER5
#5 |
MW |
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0.529 |
h60c promoter luciferase |
1 |
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| pSport6-YY1 |
MW |
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0.54 |
YY1 |
1 |
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| pMSCV-HIF-1a-dPA-GFP |
MW |
|
0.16 |
HIF-1a dPA |
1 |
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| pMSCV-HIF-1a-dPA-Thy1.1 |
MW |
|
1.3 |
HIF-1a dPA |
1 |
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| pMSCV-H60A1-GFP |
MW |
|
1.1 |
also known as H60c |
1 |
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| pMSCV-H60A1-GFP |
MW |
|
0.5 |
also known as H60c |
1 |
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| pMSCV-H60A1
delta GFP |
MW |
|
2.1 |
also known as H60c |
1 |
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| pMSCV-Rae1e-GFP |
MW |
|
0.7 |
|
1 |
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| pMSCV-CD8-FLAG-MULT1-GFP |
MW |
|
0.183 |
CD8 leader sequence followed by FLAG epitope followed by MULT1 |
1 |
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| pcDNA4-myc-his-H60A1-Ig
6X |
MW |
|
1.5 |
H60c-Fc with 6 His |
1 |
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| pCI-H60A1
w/ H60 leader |
MW |
|
1.8 |
H60c expressed with H60a leader sequence |
1 |
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| pCI-H60 |
MW |
|
0.942 |
|
1 |
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| pMSCV-H60A1
w/ CD8 leader |
MW |
|
0.361 |
H60c expressed with CD8 leader |
1 |
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| pcDNA4-Mult1-Ig |
MW |
|
0.75 |
Mult1-Fc |
1 |
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| pBS II
KS+ |
MW |
|
1.5 |
|
1 |
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| pMSCV-H60A1
w/ CD8 leader |
MW |
|
0.523 |
H60c expressed with CD8 leader |
1 |
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| pCI-GFP |
MW |
|
2.2 |
|
1 |
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| pCI-H60A1 |
MW |
|
0.68 |
H60c in pCI expression vector |
1 |
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| VECTOR
STOCK BOX 3 |
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| NAME |
FROM |
DATE |
DILUTION |
USED FOR |
#TUBES |
CAME FROM |
MORE INFO |
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| pcDNA4-myc-his-B/cL
H60A1-Ig |
|
|
0.5 |
H60c-Fc fused to H60a leader |
1 |
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| H60A1ch CD8FLAG |
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1.7 |
CD8FLAG leader fused to leaderless H60A1 |
1 |
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| H60A1ch CD8FLAG |
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1.8 |
CD8FLAG leader fused to leaderless H60A1 |
1 |
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| H60B/c chim pCI |
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0.5 |
H60B/c leader fused to leaderless H60c |
1 |
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| pMSCV-IG CD8FLAG H60A1 |
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|
0.116 |
CD8FLAG leader fused to leaderless h60A1 |
1 |
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| pMSCV-IG CD8FLAG H60A1 |
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0.325 |
CD8FLAG leader fused to leaderless h60A1 |
1 |
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| pMSCV-IRES
GFP Emp2 |
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|
0.11 ug/ul |
|
1 |
|
|
|
|
|
|
|
|
|
| pMSCV-IRES
GFP YY1 |
|
|
0.120 ug/ul |
|
|
|
|
|
|
|
|
|
|
|
| pcDNA4-Rae1-Ig |
|
|
1.34 ug/ul |
|
|
|
|
|
|
|
|
|
|
|
| pcDNA4-H60-Ig |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| pcDNA4-H60A1-Ig |
|
|
4.2 ug/ul |
|
|
|
|
|
|
|
|
|
|
|
| pMSCV-CMVpro-IRES-GFP
CD8-FLAG-Mult1 (pMCIG-CFM) |
Tim Nice |
03/08/10 |
1.25 mg/ml |
Retroviral transduction of WT Mult1encodes CD8 leader FLAG Mult1 |
1 |
|
|
|
|
|
|
|
|
|
| pMCIG-CFM
D239 |
Tim Nice |
03/08/10 |
0.8 mg/ml |
Retroviral transduction of Mult1 lacking its entire cytoplasmic domain |
1 |
|
|
|
|
|
|
|
|
|
| pMCIG-CFM
D244 |
Tim Nice |
03/08/10 |
0.3 mg/ml |
|
1 |
|
|
|
|
|
|
|
|
|
| pMCIG-CFM
D253 |
Tim Nice |
03/08/10 |
0.7 mg/ml |
|
1 |
|
|
|
|
|
|
|
|
|
| pMCIG-CFM
D253 P248A |
Tim Nice |
03/08/10 |
1 mg/ml |
|
1 |
|
|
|
|
|
|
|
|
|
| pMCIG-CFM
D253 S247A |
Tim Nice |
03/08/10 |
0.8 mg/ml |
|
1 |
|
|
|
|
|
|
|
|
|
| pMCIG-CFM
D253 S247D |
Tim Nice |
03/08/10 |
1.1 mg/ml |
|
1 |
|
|
|
|
|
|
|
|
|
| pMCIG-CFM
D253 KK240RR |
Tim Nice |
03/08/10 |
1.6 mg/ml |
|
1 |
|
|
|
|
|
|
|
|
|
| pMCIG-CFM
D298 |
Tim Nice |
03/08/10 |
1 mg/ml |
|
1 |
|
|
|
|
|
|
|
|
|
| pMCIG-CFM
KK240RR |
Tim Nice |
03/08/10 |
1.3 mg/ml |
|
1 |
|
|
|
|
|
|
|
|
|
| pMCIG-CFM
KK240RR, KLK278RLR |
Tim Nice |
03/08/10 |
0.7 mg/ml |
|
1 |
|
|
|
|
|
|
|
|
|
| pMCIG-CFM
KK240RR, KLK278RLR, KK310RR |
Tim Nice |
03/08/10 |
1.3 mg/ml |
Retroviral transduction of lysine-less (stable) Mult1 |
1 |
|
|
|
|
|
|
|
|
|
| pMCIG-CFM
SS246AA |
Tim Nice |
03/08/10 |
0.75 mg/ml |
|
1 |
|
|
|
|
|
|
|
|
|
| pMCIG-H60a |
Tim Nice |
03/08/10 |
0.8 mg/ml |
Retroviral transduction of H60a |
1 |
|
|
|
|
|
|
|
|
|
| pMCIG-H60a(ec+tm)-Mult1(ic) |
Tim Nice |
03/08/10 |
0.15 mg/ml |
Retroviral transduction of H60a-Mult1 (HMIC) |
1 |
|
|
|
|
|
|
|
|
|
| pMCIG-H60a(ec)-Mult1(tm+ic) |
Tim Nice |
03/08/10 |
0.81 mg/ml |
Retroviral transduction of H60a-Mult1 (HMTM) |
1 |
|
|
|
|
|
|
|
|
|
| pMCIG-NNL |
Tim Nice |
03/08/10 |
1.8 mg/ml |
Unknown gene with similarity to NKG2D ligands (NCBI accession: AK136660) cloned from B6 skin cDNA |
1 |
|
|
|
|
|
|
|
|
|
| pMCIG-1D*2A |
Tim Nice |
03/08/10 |
0.75 mg/ml |
poliovirus gene 2A cleaves eIF4G inhibiting cap-dependent translation |
1 |
|
|
|
|
|
|
|
|
|
| pMCIG-CFM-HA |
Tim Nice |
03/08/10 |
1 mg/ml |
CFM with c-terminal HA tag |
1 |
|
|
|
|
|
|
|
|
|
| pMCIG-CD8-HA-Mult1 |
Tim Nice |
03/08/10 |
0.5 mg/ml |
CD8 leader-HA tag-WT Mult1 |
1 |
|
|
|
|
|
|
|
|
|
| pMCIG-HuR |
Tim Nice |
03/08/10 |
1.5 mg/ml |
HuR is an RNA binding protein regulating stability |
1 |
|
|
|
|
|
|
|
|
|
| pMCIG-RPL26 |
Tim Nice |
03/08/10 |
1.5 mg/ml |
RPL26 is a ribosomal protein shown to regulate translation of p53 |
1 |
|
|
|
|
|
|
|
|
|
| pMSCV-IRES-Thy1.1
(pMIT) MYC-MARCH4 |
Tim Nice |
03/08/10 |
1.9 mg/ml |
Retroviral transduction of MARCH4 |
1 |
|
|
|
|
|
|
|
|
|
| pMIT-MYC-MARCH9 |
Tim Nice |
03/08/10 |
1.9 mg/ml |
Retroviral transduction of MARCH9 |
1 |
|
|
|
|
|
|
|
|
|
| pMIT-CD8-FLAG-Mult1-GFP |
Tim Nice |
03/08/10 |
2 mg/ml |
CFM with a c-terminal GFP fusion (GFP may be cleaved off to some extent) |
1 |
|
|
|
|
|
|
|
|
|
| pMSCV-IRES-hCD4
(pMI4) |
Tim Nice |
03/08/10 |
1.5 mg/ml |
|
1 |
|
|
|
|
|
|
|
|
|
| pMI4-BiP |
Tim Nice |
03/08/10 |
2.2 mg/ml |
ER resident chaperone BiP (GRP78) |
1 |
|
|
|
|
|
|
|
|
|
| pMI4-BiP
T37G |
Tim Nice |
03/08/10 |
0.6 mg/ml |
catalytically inactive BiP |
1 |
|
|
|
|
|
|
|
|
|
| pCMV-TAG3a
(pCT3a) |
Tim Nice |
03/08/10 |
1.2 mg/ml |
expression vector for N-terminal MYC tagging |
1 |
|
|
|
|
|
|
|
|
|
| pCT3a-PHAS1
5A |
Tim Nice |
03/08/10 |
1.3 mg/ml |
PHAS-1 is another name for 4EBP, 5A mutation stabilizes 4EBP (PHAS-1) |
1 |
John C. Lawrence Jr. lab
(UVA) |
|
|
|
|
|
|
|
| pCT3a-PHAS1
F113A |
Tim Nice |
03/08/10 |
1.2 mg/ml |
F113A stabilizes 4EBP (PHAS-1) |
1 |
John C. Lawrence Jr. lab
(UVA) |
|
|
|
|
|
|
|
| pCT3a-COP1 |
Tim Nice |
03/08/10 |
0.3 mg/ml |
encodes the COP1 E3 ubiquitin ligase |
1 |
|
|
|
|
|
|
|
|
|
| pCDNA-WT
Dynamin |
Tim Nice |
03/08/10 |
0.3 mg/ml |
|
1 |
Coscoy lab |
|
|
|
|
|
|
|
|
| pCDNA-DN
Dynamin |
Tim Nice |
03/08/10 |
1 mg/ml |
|
1 |
Coscoy lab |
|
|
|
|
|
|
|
|
| pCDNA3.1-tdTomato |
Tim Nice |
03/08/10 |
0.8 mg/ml |
|
1 |
Schlissel lab |
|
|
|
|
|
|
|
|
| pR/DE/F |
Tim Nice |
03/08/10 |
1.3 mg/ml |
Bi-cistronic dual luciferase to test for IRES activity, (Renilla luciferase-STOP codon-deadEMCV IRES-Firefly
luciferase) Clone 5' UTR into EcoRI and NcoI sites, triple ligation required |
1 |
Sarnow lab (STANFORD) |
|
|
|
|
|
|
|
|
| pR/DE/F-247bp Mult1 5'UTR for. |
Tim Nice |
03/08/10 |
1.5 mg/ml |
|
1 |
|
|
|
|
|
|
|
|
|
| pR/DE/F-247bp Mult1 5'UTR rev |
Tim Nice |
03/08/10 |
0.9 mg/ml |
|
1 |
|
|
|
|
|
|
|
|
|
| pR/DE/F-298bp Mult1 5'UTR for. |
Tim Nice |
03/08/10 |
2.4 mg/ml |
|
1 |
|
|
|
|
|
|
|
|
|
| pR/DE/F-298bp Mult1 5'UTR rev |
Tim Nice |
03/08/10 |
1.5 mg/ml |
|
1 |
|
|
|
|
|
|
|
|
|
| pR/DE/F-600bp Mult1 5'UTR |
Tim Nice |
03/08/10 |
1 mg/ml |
|
1 |
|
|
|
|
|
|
|
|
|
| pR/DE/F-EMCV IRES |
Tim Nice |
03/08/10 |
1.6 mg/ml |
|
1 |
|
|
|
|
|
|
|
|
|
| pGL3-247bp
Mult1 5'UTR |
Tim Nice |
03/08/10 |
1.2 mg/ml |
pGL3 control plasmid with Mult1 UTR cloned adjacent to luciferase |
1 |
|
|
|
|
|
|
|
|
|
| pGL3-298bp
Mult1 5'UTR |
Tim Nice |
03/08/10 |
0.5 mg/ml |
pGL3 control plasmid with Mult1 UTR cloned adjacent to luciferase |
1 |
|
|
|
|
|
|
|
|
|
| pGL3-Mult1
3'UTR |
Tim Nice |
03/08/10 |
0.5 mg/ml |
pGL3 control plasmid with Mult1 UTR cloned adjacent to luciferase |
1 |
|
|
|
|
|
|
|
|
|
| pGL3-298bp
Mult1 5'UTR + Mult1 3'UTR |
Tim Nice |
03/08/10 |
2 mg/ml |
pGL3 control plasmid with Mult1 UTR cloned adjacent to luciferase |
1 |
|
|
|
|
|
|
|
|
|
| pGL3-Mult1
3'UTR D1 |
Tim Nice |
03/08/10 |
1 mg/ml |
first 1kb of Mult1 3'UTR |
1 |
|
|
|
|
|
|
|
|
|
| pGL3-Mult1
3'UTR D2 |
Tim Nice |
03/08/10 |
1.2 mg/ml |
middle 0.5kb of Mult1 3'UTR |
1 |
|
|
|
|
|
|
|
|
|
| pGL3-Mult1
3'UTR D3 |
Tim Nice |
03/08/10 |
1.2 mg/ml |
last 0.5kb of Mult1 3'UTR |
1 |
|
|
|
|
|
|
|
|
|
| pGL3-Mult1
3'UTR D12 |
Tim Nice |
03/08/10 |
1.1 mg/ml |
first 1.5kb of Mult1 3'UTR (D1 and D2
combined) |
1 |
|
|
|
|
|
|
|
|
|
| pGL3-Mult1
3'UTR D23 |
Tim Nice |
03/08/10 |
1 mg/ml |
last 1.5kb of Mult1 3'UTR (D2 and D3
combined) |
1 |
|
|
|
|
|
|
|
|
|
| pCGN-HA-hMARCH1 |
Tim Nice |
03/08/10 |
1.1 mg/ml |
|
1 |
Coscoy lab |
|
|
|
|
|
|
|
|
| pCGN-HA-hMARCH2 |
Tim Nice |
03/08/10 |
0.5 mg/ml |
|
1 |
Coscoy lab |
|
|
|
|
|
|
|
|
| pCGN-HA-hMARCH3 |
Tim Nice |
03/08/10 |
1 mg/ml |
|
1 |
Coscoy lab |
|
|
|
|
|
|
|
|
| pCGN-HA-hMARCH4 |
Tim Nice |
03/08/10 |
1.2 mg/ml |
|
1 |
Coscoy lab |
|
|
|
|
|
|
|
|
| pCGN-HA-hMARCH8 |
Tim Nice |
03/08/10 |
1.1 mg/ml |
|
1 |
Coscoy lab |
|
|
|
|
|
|
|
|
| pCGN-HA-hMARCH9 |
Tim Nice |
03/08/10 |
1 mg/ml |
|
1 |
Coscoy lab |
|
|
|
|
|
|
|
|
| pLMP |
Tim Nice |
03/08/10 |
2 mg/ml |
|
1 |
Barton lab (via open
biosystems) |
|
|
|
|
|
|
|
| pCDNA3.1-GFP |
Manali |
03/08/10 |
0.6 and 1 mg/ml |
pCDNA3.1 with GFP replacing Neomycin selection gene (XmaI
and BstBI sites) |
pCDNA3.1 with GFP
replacing Neomycin selection gene (XmaI and BstBI sites) |
|
|
|
|
|
|
| pCDNA3.1-GFP
Mult1 |
Manali |
03/08/10 |
0.5 mg/ml |
Mult1 cloned into AflII and NotI sites |
2 |
|
|
|
|
|
|
|
|
|
| pCDNA3.1-GFP
Mult1+Mult1 3' UTR |
Manali |
03/08/10 |
0.7 and 0.2 mg/ml |
Mult1 3' UTR cloned into NotI and XhoI sites |
2 |
|
|
|
|
|
|
|
|
|
| pCDNA3.1-GFP
Mult1+GYK 3' UTR |
Manali |
03/08/10 |
0.3 and ? mg/ml |
GYK 3'UTR cloned into NotI and XhoI sites |
2 |
|
|
|
|
|
|
|
|
|
| pSicoR
mDICER shRNA |
Tim (via Nataliya) |
03/28/10 |
0.35 mg/ml |
DICER knockdown |
1 |
? |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| VECTOR
STOCK BOX 4 |
|
|
|
|
|
|
|
| NAME |
FROM |
DATE |
DILUTION |
USED FOR |
#TUBES |
CAME FROM |
MORE INFO |
|
|
|
|
|
|
|
| MSCV-pm
Cancer 1000 |
Tim Nice |
03/28/10 |
2.9 mg/ml |
shRNA screen |
1 |
S. Elledge |
|
|
|
|
|
|
|
|
| MSCV-pm
Human Kinase array I |
Tim Nice |
03/28/10 |
2.1 mg/ml |
shRNA screen |
1 |
S. Elledge |
|
|
|
|
|
|
|
|
| MSCV-pm
Human Kinase array II |
Tim Nice |
03/28/10 |
1.8 mg/ml |
shRNA screen |
1 |
S. Elledge |
|
|
|
|
|
|
|
|
| MSCV-pm
Human Ub array I |
Tim Nice |
03/28/10 |
2.3 mg/ml |
shRNA screen |
1 |
S. Elledge |
|
|
|
|
|
|
|
|
| MSCV-pm
Human UB array II |
Tim Nice |
03/28/10 |
1.8 mg/ml |
shRNA screen |
1 |
S. Elledge |
|
|
|
|
|
|
|
|
| pPGK-Hyg |
Sang-Ho |
11/20/06 |
|
Marker Cassette |
|
Dr. Sohn (Winoto Lab) |
hygromycin resistance
gene cassette containing plasmid, positive selection marker for Tg mouse |
|
| pBS-SK |
Sang-Ho |
|
|
cloning vector |
|
Lily Zhang |
parental vector for Vg
gene KO and targeting |
|
|
|
|
|
| pTKNeoLoxP
5' arm 3' arm |
Sang-Ho |
11/20/06 |
|
Knock in |
|
Lily Zhang |
basal structure of Knock
in construct for Vγ4 Vγ3 bearing upstream of L4 and downstream of
V3 |
|
| pKSTKneoLoxP |
Sang-Ho |
11/21/06 |
|
Marker Cassette |
|
Lily Zhang |
Backbone of pTKNeoLoxP 5'
arm 3' arm |
|
|
|
|
|
| PGKneoF2L2DTA |
Sang-Ho |
11/21/06 |
|
Marker Cassette |
|
Dr. Phil Soriano |
diphtheria toxin A (DTA)
for negative selection marker, not need any drug for the selection, for RMCE |
|
| pCre/FLPe |
Sang-Ho |
04/10/08 |
|
recombinase |
|
Dr. Matthias Lauth |
NLS-Cre and NLS-Flpe
recombinase expression plasmid for Recombinase mediated cassette exchange
(RMCE) |
| pFLPe |
Sang-Ho |
06/25/08 |
|
recombinase |
|
|
made from pCre/FLPe by
deleting Cre gene (PstI-BamHI fragment) |
|
|
|
| pMC-Cre |
Sang-Ho |
04/10/08 |
|
recombinase |
|
Lily Zhang |
|
|
|
|
|
|
|
|
| pTKLoxPNeo
FrtHyg 5' arm 3' arm |
Sang-Ho |
01/11/07 |
|
Knock in |
|
|
Final construct for
Knock-in for pL4 pL3 KO |
|
|
|
|
|
| pBS L4V4 |
Sang-Ho |
11/21/06 |
|
unarranged mouse genome |
|
Lily Zhang |
intermedate construct |
|
|
|
|
|
|
|
| pBS L4V4
L3V3 |
Sang-Ho |
11/21/06 |
|
unarranged mouse genome |
|
Lily Zhang |
Initial clone of Vγ4
Vγ3 from BAC to pBS for subcloning |
|
|
|
|
| pBS V4V3
no arm |
Sang-Ho |
11/21/06 |
|
unarranged mouse genome |
|
Lily Zhang |
intermediate construct,
Lack of 5' arm and 3' arm from pBS L4V4 L3V3 |
|
|
|
| pBS
Vγ2 |
Sang-Ho |
11/21/06 |
|
unarranged mouse genome |
|
Lily Zhang |
intermedate construct |
|
|
|
|
|
|
|