 |
Te-Wen Lo - Post-Doctoral Researcher 131 Koshland Hall Mailing Address: |
| Research Summary |
| Dosage compensation is the mechanism by which the expression of X-linked genes are equalized between males with a single X chromosome and females with two. Comparisons between flies, worms, and mammals indicate that dosage compensation occurs differently between these organisms. In C. elegans, the most widely studied nematode species, transcription levels from each of the X chromosomes in the XX hermaphrodite is reduced by half to equal transcription levels of the XO male. This reduction is controlled by a developmental signal that determines the ratio of X chromosomes to sets of autosomes. This X:A signal initiates a cascade of sex determination and dosage compensation processes. To further understand the process of dosage compensation, we hope to examine the dosage compensation pathway in C. briggsae, a sister nematode species. More specifically, we hope to determine whether both the X:A signal elements and the dosage compensation complex are conserved. We are taking a reverse genetic approach to identify mutants in known components of the dosage compensation pathway. Phenotypic and molecular characterization of these mutants will allow us to determine if dosage compensation mechanisms in C. briggsae are similar to those in C. elegans. Our analyses will serve as a first step toward understanding not only the process in C. briggsae, but also provide insight toward the evolution of the X:A signal and dosage compensation mechanisms. |
| Publications/Presentations |
Wood AJ*, Lo TW*, Zeitler B*, Pickle CS, Ralston EJ, Lee AH, Amora R, Miller JC, Leung E, Meng X, Zhang L, Rebar EJ, Gregory PD, Urnov FD, Meyer BJ. (2011) Targeted genome editing across species using ZFNs and TALENs. Science 333, 307. *These authors contributed equally to this work. Lo, T., Bennett, D.C., Sasson., I.E., Goodman, S.J., Stern, M.J. (2010). C. elegans FGF Receptor Signaling Can Occur Independently of the Multi-substrate Adaptor Substrate FRS2. Genetics. 185(2):537-47. Lo, T., Branda, C.S., Huang, P., Sasson., I.E., Goodman, S.J., Stern, M.J. (2008). Different isoforms of the C. elegans FGF receptor are required for attraction and repulsion of the migrating sex myoblasts. Developmental Biology. 318:268-275. Lo, T. , Stern, M.J. (2007, June). C. elegans FGF Receptor Signaling can Occur via Direct Binding of the SEM-5/Grb2 Adaptor Protein. Sixteenth International Worm Meeting, Los Angeles, CA. Lo, T. , Branda, C.S., Huang, P., Sasson, I.E., Goodman, S.J., Stern, M.J. (2004, June). EGL-15 FGF Receptor Isoforms Play Different Roles in SM Migration. East Coast Worm Meeting, New Haven, CT. Pepper, A.S., Lo, T. , Killian, D.J., Hall, D.H., Hubbard, E.J.A. (2003). The establishment of Caenorhabditis elegans germline is controlled by overlapping proximal and distal somatic gonad signals. Developmental Biology. 259: 336-350. Lo, T . & Adolph, K. E. (2001, June). Depth information is privileged: The case of friction. International Conference on Perception and Action, Storrs, CT. Lo, T . & Hubbard, E.J.A. (2000, June). GLP-1 Proximal (Pro) Mutants. East Coast Worm Meeting, Atlanta, GA. Lo, T. , Avolio, A. M., Massop, S. A., & Adolph, K. E. (1999). Why toddlers don't perceive risky ground based on surface friction. In M. A. Grealy & J. A. Thompson (Eds.), Studies in Perception and Action V. NJ: Erlbaum, pp. 231-235. |
| Education |
| 2000 - 2007 Yale University M.Phil. in Genetics Ph.D. in Genetics 1998 - 2000 1994 - 1998 |