Cathy Pickle - Research Technician

131 Koshland Hall
(510) 643-5583
cpickle@berkeley.edu

Mailing Address:
University of California Berkeley
16 Barker Hall, MC 3204
Berkeley, CA 94720-3204

 

 

 

 

 

 

 

 

Research Summary
Assist in developing and implementing investigative procedures to accomplish the lab's research goals. Independently, plan and conduct detailed molecular biology experiments, perform complex analyses, and interpret experimental results. Study mechanism by which number of X chromosomes is counted in C. elegans to determine sex. Specifically studying how one X chromosome signal element, an RNA-binding protein, controls the alternate splicing of the master sex determination switch gene, xol-1. Utilize radioisotopes to make radio-labeled RNA sequences, and perform RNA/protein cross-linking experiments to determine where the protein binds within a specified region of the intron. Design and clone gfp- and lac-Z constructs, with and without the specified intronic sequence, to put into C. elegans and test for efficacy. Collaborate with the primary investigator in the design of future experiments.

Organize, update and maintain the lab's antisera collection computer database, which includes keeping track of current live animals, recording all animal bleeds and any subsequent purifications and their uses, and keeping an organized freezer inventory of all antisera stocks. Interact with and lend technical support to students, participate in technical discussions, and present research progress reports at lab meetings. Improve existing laboratory protocols. Function as the lab's Safety Officer, which requires maintaining a safe work environment and training new personnel in lab safety procedures. Member of Building Emergency Staff: Floor Monitor-Floor EL. Will be supervising an undergraduate research assistant.
Publications/Presentations

(Note: C. S. Pickle a.k.a. C. S. Baden)

Jans, J. Gladden, J. M., Ralston, E. J., Pickle, C. S., Michel, A. H., Pferdehirt, R. R., Eisen, M. B., Meyer, B. J. 2009.  A condensin-like dosage compensation complex acts at a distance to control expression throughout the genome.   Genes & Development 23 (5), 602-618.

John Gately Luz, Christian A. Hassig, Catherine Pickle, Adam Godzik, Barbara J. Meyer, and Ian A. Wilson (2003): XOL-1, primary determinant of sexual fate in C. elegans, is a GHMP kinase family member and a structural prototype for a class of developmental regulators. Genes & Dev. 17: 977 - 990.

Kathleen Y. Lee, Catherine S. Baden, William J. Howie, John Bedbrook and Pamela Dunsmuir (1997): Post-transcriptional gene silencing of ACC synthase in tomato results from cytoplasmic RNA degradation. The Plant Journal 12(5), 1127-1137.

Robin Hightower, Catherine Baden, Eva Penzes, and Pamela Dunsmuir (1994): The expression of cecropin peptide in transgenic tobacco does not confer resistance to Pseudomonas syringae pv tabaci. Plant Cell Reports 13: 295-299.

Robin Hightower, Cathy Baden, Eva Penzes, Peter Lund and Pamela Dunsmuir (1991): Expression of antifreeze proteins in transgenic plants. Plant Molecular Biology 17: 1013-1021.

Comai L, Baden CS, Harada JJ (1989): Deduced Sequence of a Malate Synthase Polypeptide Encoded by a Subclass of the Gene Family. J Biol Chem 264(5): 2778-2782.

Comai L, Dietrich RA, Maslyar DJ, Baden CS, Harada JJ (1989): Coordinate Expression of Transcriptionally Regulated Isocitrate Lyase and Malate Synthase Genes in Brassica napus L. The Plant Cell 1: 293-300.

Harada JJ, DeLisle AJ, Baden CS, Crouch ML (1989): Unusual Sequence of an Abscisic Acid-Inducible mRNA which Accumulates Late in Brassica napus Seed Development. Plant Molecular Biology 12: 395-401.

Harada JJ, Dietrich RA, Comai L, Baden CS (1988): Regulation of Gene Expression During Seed Germination and Postgerminative Development. In: Verma DPS, Goldberg RB (ed) Plant Gene Research, Temporal and Spatial Regulation of Plant Genes. Springer-Verlag Wien New York, pp 27-39.

Harada JJ, Baden CS, Comai L (1988): Spatially Regulated Genes Expressed During Seed Germination and Postgerminative Development are Activated During Embryogeny. Mol Gen Genet 212: 466-473.
Education
1979 - 1983
University of California, Davis
B.S. Genetics