Oligo Calculator

Enter Oligo Sequence in Box
Length Melting Temperature (Tm) °C
%GC content Molecular Weight: daltons (g/M)
OD of 1 is equal to nanoMolar.

Required Tm= Known Tm + 16.6log (required salt concentration / 0.195M), where the Known Tm is calculated from 0.195M using the OligoCalculator.