[GdmCl] and [Urea] Calculator
Buffer 
Refractive Index
 Denaturant 
Refractive Index
 Warn me if above solubility (25oC)

           

[GdmCl]           [Urea] 
Property Urea GdmCl
Molecular Weight 60.056 g/mol 95.533 g/mol
Solubility, 25oC 10.49 M 8.54 M
d/doa 1 + 0.2658W + 0.0330 W2 1 + 0.2710W + 0.0330 W2
Molarityb 117.66(Dh) + 29.753(Dh)2 + 185.56(Dh)3 57.147(Dh) + 38.68(Dh)2 - 91.60(Dh)3
Grams of Denaturant/Gram of H2O    
6 M 0.495 g 1.009 g
8 M 0.755 g 1.816 g
10 M 1.103 g --
aW is the weight fraction of denaturant in the solution, d is the density of the solution and do is the density of H2O.
bDh is the difference in refractive index between the denaturant solution and water (or buffer) at the sodium D line. The equation for urea solutions is based on data from Warren and Gordon, and the equation for GdmCl is from Nozaki.
 
Weight % Mol/L Mol/kg H2O
4.7200.500--
5.693 0.603 0.632
10.299 1.105 1.202
20.052 2.209 2.625
23.767 2.645 3.263
29.377 3.318 4.354
34.688 3.975 5.559
38.197 4.418 6.469
42.247 4.940 7.656
42.700 5.000 --
46.741 5.531 9.186
50.040 5.973 10.484
50.236 6.000 --
52.791 6.348 11.704
54.759 6.619 12.669
54.847 6.632 12.714
57.528 7.005 14.177
60.340 7.399 15.925

Original tables of data for making denaturant solutions. [PDF]