• Yeast
  1. Chromatin IP's (Meluh)
  2. Chromatin IP's (Megee)
  3. Chromatin IP's (Mechanical Lysis)
  4. Chromatin IP's (9/2009)
  5. Flow Cytometry for Yeast: Cell Preparation
  6. Arresting Yeast
  7. High Efficiency Yeast Transformation (Gietz Long Protocol)
  8. Quick Yeast Tranformation (Gietz Short Protocol)
  9. Yeast Gene Knockouts
  10. Met15
  11. Plasmid Isolation from Yeast
  12. Sporulation
  13. Frogging
  14. Frozen Permanents
  15. Sending Strains
  16. YAC Transfer by KAR1
  17. In Vitro Cohesin Binding Assay
      
• Microscopy
  1. DAPI Staining of Yeast Cells
  2. FISH for Yeast
  3. Quick Fixation of Yeast Cells for GFP Fluorescence
  4. Indirect Immunofluorescence for Yeast I
  5. Indirect Immunofluorescence for Yeast II: modifications for GFP fix.
  6. Yeast Chromatin Spreads
  7. Yeast Chromatin Spreads II (Guacci)
  8. Meiotic Spreads
  9. Meiotic Synchrony
     
     
• DNA/RNA

1. Best PCR
2. DNA Fragment Purification: Low Melting Agarose
3. DNA Fragment Purification: Glass Wool
4. Isolation of High MW Yeast DNA
5. SDS Hybridization Solutions for Southerns
6. Yeast RNA Isolation
7. Northern Blots
8. Pulse Field Electrophoresis

• Protein
  1. Yeast Protein Prep (No Boil)
  2. Yeast Protein Prep (Boil)
  3. SDS Page (Biorad)
  4. Western
  5. Western Blot with BSA
  6. Carbonate Solution For Western Blot
  7. Silver Stain
     
     
• Reagents and Equipment
  1. Lab Antibodies: Conc. for Western & IF
  2. ChIP Primers for Yeast Chromosomes
  3. Alpha Factor
  4. Ampicillin
  5. Oxalyticase
  6. Prep of Protein A Sepharose Beads
  7. Prep of Glass Beads for Yeast Lysis
  8. RNAse
  9. Salmon Sperm DNA
  10. SCE
  11. Spectrophotometers: Instructions for Lab and Upstairs
  12. Zymolyase
  13. 10X TBE
  14. 20X SSPE
  15. EDTA
      
      
• Media
  1. YPD Plates
  2. YPD Media (1L)
  3. YP Media (Large Batch)
  4. YPG Plates
  5. 2X N
  6. 2X YP Media
  7. Adenine Stock
  8. 2X Agar for bacteria and yeast
  9. B plates
  10. Supplements
  11. 20% Dextrose
  12. 30% glycerol
  13. Dropout Plates (500 ml)
  14. Dropout Plates (3L)
  15. 0.3% KOAc
  16. L-Broth
  17. L-Broth + Ampicillin
  18. L-Plates
  19. M9 Pro Thr Plates (Bacterial Media for Growing pDK plasmids)
  20. Benomyl Plates
  21. 5-FOA Plates
  22. G418 YEP plates
  23. G418 Dropout plates
  24. Clonat Plates
  25. Lead Plates
      
      
• Computer

1. Quantitation of EtBr Gels (Image Quant & Excel)

• Bacteria

1. Frozen competent E. coli cells
2. Electroporating E. coli
3. Site-directed mutagenesis with oligos
4. STET preps for rapid recovery of plasmid DNA
5. Frozen Permanents
6. Sending Strains

• Microtubules
  1. Minichromosome-Microtubule Binding Assay
  2. Making Microtubule Seeds
  3. Cycling Tubulin
  4. Mt Staining by Indirect Immunofluorescence
  5. Ron Vale Mt Polymerization Buffer