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Frozen Permanents

YEAST
1) grow culture to stationary in YEPD
2) Add 0.8ml of culture to 0.8ml of 30% glycerol in labelled freezer vials
3) vortex and place at -80^oC
4) for strains with plasmids, pick a colony from a selective plate, grow to stationary in YEPD and freeze in glycerol. streak out on selective plates

BACTERIA
1) grow cells overnight in LB or LB plus appropriate drug
2) add 0.8ml of culture to 0.8ml of 30% glycerol in labelled freezer vial.
3) vortex and place at -80^oC

Koshland Web Site/Methods
Frozen Permanents
Frozen Permanents YEAST 1) grow culture to stationary in YEPD 2) Add 0.8ml of culture to 0.8ml of 30% glycerol in labelled freezer vials 3) vortex and place at -80^oC 4) for strains with plasmids, pick a colony from a selective plate, grow to stationary in YEPD and freeze in glycerol. streak out on selective plates BACTERIA 1) grow cells overnight in LB or LB plus appropriate drug 2) add 0.8ml of culture to 0.8ml of 30% glycerol in labelled freezer vial. 3) vortex and place at -80^oC
Comments:
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