20X SSPE BUFFER

1. 3 Liters
   525.9g NaCl sodium chloride
   82.8g NaH₂PO₄ sodium phosphate monobasic
   28.2g EDTA powder FW=372
   
2. bring up to 2400mls with H₂O
   
3. add NaOH to pH 7.4 (~27mls/liter of 10N NaOH)
   
4. autoclave for 20 min

1. 2 Liters
   350.6g NaCl sodium chloride
   55.2g NaH₂PO₄ sodium phosphate monobasic
   18.8g EDTA powder FW=372
   
2. bring up to 1600mls with H₂O
   
3. add NaOH to pH 7.4 (~27mls/liter of 10N NaOH)
   
4. autoclave for 20 min
   

1. 1 Liters
   175.3g NaCl sodium chloride
   27.6g NaH₂PO₄ sodium phosphate monobasic
   9.4g EDTA powder FW=372
   
2. bring up to 800mls with H₂O
   
3. add NaOH to pH 7.4 (~27mls/liter of 10N NaOH)
   
4. autoclave for 20 min