CATHY'S NO BOIL YEAST EXTRACTS

1. Cells to mid log/ need ~ 5OD. Spin down and wash w/ 1mL H₂O
   
2. Transfer to 1.5mL round bottom screw cap tube
   
3. add 200 ul SDS sample buffer + (5% BME, 50mM NaF, 1mM PMSF)-add to stock just before use; + 200ul glass beads
   
4. Beat 90sec (setting 9) in cold room and spin down 2', 4^oC.
   
   Sample buffer: 4% SDS 5mL+
   80mM Tris Cl 6.8
   Final 10% glycerol
   10mM EDTA
   0.2% BFB