CARBONATE WESTERN TRANSFER SOLUTIONS
Dunn, Anal. Biochem. 1986: 157

	1.5L		1.0L
10mM NaHCO3	1.26g	chill to 4oC	0.84g
3mM Na2CO3	0.477g	pH ~9.9	0.318g
in 20% MeOH	300mL		200mL

1. Blot: no need to presoak gell.
   Transblot: 1-2H @ 1A keep T<35^oC
   Miniblot: 1H @ 250mA
2. Assemble wet sandwich on black side:
   black side holder
   brillo pad
   3MM paper
   gel
   nitrocellulose
   3MM
   brillo
   white side holder