Koshland Web Site/Methods
Supplements
100X stocks (Gottschling)
Sup [Stock] [Final] /5 ml /30 ml
Ade* 1 mg/ml 10µg/ml 50 µl .3 ml
His 5 mg/ml 50 µg/ml 50 µl .3 ml
Leu 10 mg/ml 100 µg/ml 50 µl .3 ml
Lys 10 mg/ml 100 µg/ml 50 µl .3 ml
Trp* 10 mg/ml 10 mg/ml 50 µl .3 ml
Met 5 mg/ml 50 µg/ml 50 µl 3 ml

Hartwell Drop out supplements

Sup [Stock] [Final] /5 ml /30 ml /40 ml
Sporulaton Media
Ade* 1 mg/ml 20 µg/ml 100 µl 600 µl 800 µl
His 10 mg/ml 20 µg/ml 10 µl 60 µl 20 µl
Leu 20 mgl/ml 80 µg/ml 20 µl 120 µl 40 µl
Lys 10 mg/ml 120 µg/ml 60 µl 360 µl 120 µl
Trp * 10 mg/ml 80 µg/ml 40 µl 240 µl 80 µl
Ura* 1 mg/ml 35 µg/ml 175 µl 1050 µl 350 µl
Comments:

*Filter sterilize and store at room temperature otherwise will ppt. Others can be autoclaved.

For Uracil it may be easier to add 17.5 mg to 500 ml.
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