Koshland Web Site/Methods
M9 PRO THR PLATES
300 ml 2% agar
100 ml 4X M9
4 ml 20% dextrose
4 ml 0.1M MgSO4
0.1 ml B1 (0.1%)
4 ml Threonine (100X Stock 4 mg/ml)
4 ml Proline (100X Stock 4 mg/ml)

4XM9

56 gm. Na2HPO4 (Anhydrous)
24 gm. KH2PO4 (Anhydrous)
8 gm. NH4Cl
4 gm. NaCl
Bring to 2 liters with dH2O.
Aliquot 400 ml / bottle and autoclave.

Comments:
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