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G418 Dropout (final conc. G418 - 200 µg/ml

1) Dissolve in 400 ml of H₂0 and sterilize
     1.7 g yeast nitrogen base without (NH₄)₂SO₄ or aa
     1.0 g monosodium glutamate
     2.0 g droupout powder
     100 mg G418 (G418 sulfate, Invitrogen Gibco 11811-031)

2) 100 ml 20% dextrose
3) Mix 1 and 2
4) add 500 ml 4% agar

Koshland Web Site/Methods
G418 Dropout PLATES (500ml)
G418 Dropout (final conc. G418 - 200 µg/ml 1) Dissolve in 400 ml of H₂0 and sterilize 1.7 g yeast nitrogen base without (NH₄)₂SO₄ or aa 1.0 g monosodium glutamate 2.0 g droupout powder 100 mg G418 (G418 sulfate, Invitrogen Gibco 11811-031) 2) 100 ml 20% dextrose 3) Mix 1 and 2 4) add 500 ml 4% agar
Comments:
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