Untitled Document

1. Mix:

200 ml 2X -N media (200 ml H₂O + 3.5 g of YNB w/ AmSO₄ w/o AA)
25 mg of uracil
0.5 g of 5 FOA
50 ml of Dextrose
0.7 gm of minus ura powder or appropriate supplements

2. Stir at 50^oC. FOA will take awhile to go into solution.
3. Add 250 ml of melted 4 % agar.

Koshland Web Site/Methods
5-FOA PLATES
1. Mix: 200 ml 2X -N media (200 ml H₂O + 3.5 g of YNB w/ AmSO₄ w/o AA) 25 mg of uracil 0.5 g of 5 FOA 50 ml of Dextrose 0.7 gm of minus ura powder or appropriate supplements 2. Stir at 50^oC. FOA will take awhile to go into solution. 3. Add 250 ml of melted 4 % agar.
Comments:
Koshland Lab Home Page \| Berkeley Faculty Page