2X N MEDIA
1600 mls
5.8g yeast nitrogen base; without amino acids or ammonium sulfate
20g ammonium sulfate
bring up to 1600 mls with H₂O
mix
divide into 200ml aliquots
label and autoclave for 20 min

2XN
26.8g Bio-101 YNB
1600 ml H2O
no ammonium sulfate
200ml/bottle

2XN
YNB without ammonium sulfate
use 6.8g YNB and 20g ammonium sulfate