SDS Hybridization Solution

	100mls
7 % SDS	7g of Electrophoresis Grade SDS
1 % BSA	3.3 mls of 30% BSA frac. V
1mM EDTA	0.2 mls of 0.5 M EDTA pH 8.0
Phosphate Buffer	50 mls of 2X NaHPO4 pH 7.2
	= 134g of Na2HPO4.7H2O + 4 mls 85% H3PO4 / Liter Don't Always Believe what is Written by Church and Gilbert !!!

SDS Hyb. Wash

Buffer #1, 1 liter

• 50 g of "cheap" SDS
• Add ~700mls H₂O place on hot plate/stirrer, when "in" solution allow to cool while stirring then add :
• 40 mls of 2X NaHPO₄ pH 7.2 = (134g of Na₂HPO₄.7H₂O + 4 mls 85% H₃PO₄ / Liter)
• 2 mls of 0.5 M EDTA
• 16.6 mls 30% BSA; bring up to 1 liter pre-heat at 65^oC
• WASH 2X's 15 minutes each at 65^oC using ~500mls in a medium Tupperware container

Buffer #2, 1 liter

• 50 mls of 20% "cheap" SDS or 10g SDS
• 40 mls of 2X NaHPO₄ pH 7.2 = (134g of Na₂HPO₄.7H₂O + 4 mls 85% H₃PO₄ / Liter)
• 2 mls of 0.5 M EDTA pre-heat at 65^oC
• WASH 2X's ~5-10 minutes each at 65^oC (check by hand monitor)
• seal in Saran Wrap®