Movie 1. Three-dimensional (3D) live imaging of stage 8 to 9 follicle expressing Indy-GFP to mark follicle epithelia plasma membranes, imaged at the basal surface. The follicle epithelium is seen moving perpendicular to the long A-P axis but stops towards the end of the movie when border cell migration has begun. Movie length is 4 hours with 2-min time points.
Movie 2. 4D live imaging of an ovariole expressing His2Av-mRFP and Indy-GFP with 87 um maximal projection. In stage 6 and 7 follicles, large polyploid germline nuclei (red) are seen rotating in concert with the follicle epithelium (green), while stage 4 and stage 9 follicles do not rotate. Note also that the stage 6 and stage 7 follicles are rotating with opposite chirality. Movie length is 3 hours with 5-min time points.
Movie 3. 4D live imaging of stage 8 follicle expressing myristolated mRFP (red) in follicle epithelia and collagen IV–GFP (green) to highlight the fibrillar collagen IV matrix, imaged at the basal surface with 26 µm maximal projection. Follicle cells can be seen moving past static collagen IV fibrils. Note that intracellular collagen IV puncta can be seen moving within follicle cells against static collagen IV fibrils within the basement membrane. Merge is to the left, collagen IV-GFP is in the upper right, myr-mRFP is in the bottom right. Movie length is 38 min with 2-min time points.