Professor of the Graduate School Division of Biochemistry, Biophysics and Structural Biology
We are constructing a plasmid vector that will allow insertion of any gene of interest at a defined site on the Bacillus anthracis chromosome. We are characterizing a new class of B. cereus/anthracis phages that may be used for bio-control.
We are constructing an integration vector--a plasmid that can insert in the B. anthracis chromosome--that will allow us to express particular antigens of B. anthracis at a high level. The new phages that we are characterizing are extremely virulent. We intend to determine why this is so.
Listeria monocytogenes tyrosine phosphatases affect wall teichoic acid composition and phage resistance.
Ran Nir-Paz, Marcel R. Eugster, Einat Zeiman, Martin J. Loessner and Richard Calendar.
FEMS Microbiology Letters 326:151-160, 2012.
Complete nucleotide sequence and molecular characterization of Bacillus phage TP21-L and its relatedness to other phages with the same name. Jochen Klumpp, Richard Calendar, and Martin J. Loessner. Viruses 2:961-971, 2010.
Comparative genome analysis of Listeria bacteriophages reveals extensive mosaicism, programmed translational frameshifting, and a novel prophage insertion site. J. Dorscht, J. Klumpp, R. Bielmann, M. Schmelcher, Y. Born, M. Zimmer, R. Calendar and M. J. Loessner. J. Bacteriol 191:7206-7215, 2009.
Killed but metabolically active Bacillus anthracis vaccines induce broad and protective immunity against anthrax. J. Skoble, J. W. Beaber, Y. Gao, J. A. Lovchik, L. E. Sower, W. Liu, W. Luckett, J. W. Peterson, R. Calendar, D. A. Portnoy, C. R. Lyons, and T. W. Dubensky. Infection and Immunity 77:1649-1673, 2009.
Terminally Redundant, non-permuted Genome of Listeria Bacteriophage A511: a Model for the SPO1-like Myoviruses of Gram-Positive Bacteria. J. Klumpp, J. Dorscht, R. Lurz, R. Bielmann, M. Wieland, M. Zimmer, R. Calendar, and M. J. Loessner. J. Bacteriol. 190:5753-5765, 2008.
The Bacteriophages. R. Calendar, Editor. Oxford University Press, New York, 2006.
Bacteriophage Biology. R. Calendar and R. B. Inman. In Phages: Their Role in Bacterial Pathogenesis and Biotechnology," Ed. M. Waldor, D. Friedman and D. Court, American Society for Microbiology, Washington, D.C., 2005.
Construction, Characterization, and Use of two Listeria monocytogenes site-specific phage integration vectors. P. Lauer, M. Chow, M. J. Loessner, D. Portnoy, and R. Calendar. J. Bacteriol. 184: 4177-4186, 2002.
Gene insertion and replacement in Schizosaccharomyces pombe mediated by the Streptomyces bacteriophage phiC31 site-specific recombination system Lynn C. Thomason, Richard Calendar, and David W. Ow. Molecular Genetics and Genomics 265:1031-1038, 2001.
Last Updated 2012-08-07