Single Tube Submission

Please follow these guidelines when preparing your samples:

Each sample tube (1.5ml tubes preferred) should contain one primer and one template at the quantities specified below:

Please note that the values listed for the primer are quantities, not concentrations. 

  • ssDNA plasmid:  0.25μg DNA/5kb    + 2 pmol primer in 13 µl
  • dsDNA plasmid:  0.5μg DNA/5kb      + 8 pmol primer in 13 µl
  • PCR products:    2-4ng DNA/100bp  + 8 pmol primer in 13 µl

 

If requesting one of our stock primers, put a large note in red on your order form, and submit samples in 10uL total volume.

Template: Please be sure that you do not have SALTS, PROTEIN, DETERGENTS, RNA, CHROMOSOMAL DNA, ETHANOL, or EDTA.

Quantify your products on an agarose gel or use a NanoDrop. Please let us know if your products are at a low concentration and/or are larger than 10kb.

Primers: Our default annealing temperature is 50oC.  Please let us know if this is not compatible with your primer(s).

Order forms can be filled out and printed from this web site by following the Order Form link