Plasmid Prep

Plasmid Prep:

This service utilizes a magnetic bead technology for high-throughput purificatin fo plasmid DNA from E. Coli.   It works for both high and low copy plasmid.

You will need to contact us the day before submitting your cultures to confirm that someone will be available to process your samples.

To take advantage of this, you will need to submit:

1. Stop by 310 Barker Hall to pick up a 96-well 2.2 ml deep well culture block and a gas permeable seal.
2. Up to 95 samples (reserving well A1 for our control).
3. Cultures grown in 1mL of 2YT medium containing appropriate antibiotic in a deep well culture block.
4. Bacteria can be grown from either a single colony picked from a plate or from a glycerol stock. Cells should be grown with shaking (300rpm) at 37 ^oC until absorbance at  OD600_nm=0.4 to 0.6.
5. We are not able to accept these plates on the weekend.
6. Once the plasmid cleanup is complete, we can perform the sequencing reaction for you.  Please indicate clearly which primer(s) you need us to use, and provide sufficient amount of primer (unless it is one of the following primers: T7F, T7R, M13F, M13R, T3, SP6, which we have in stock).
7. Please email a copy of the Full Plate Sequencing Form to dnaseq@berkeley.edu