PCR Clean Up

PCR Clean Up:

By Plate:  $36/plate

NOTE: We elute PCR products in 40ul of H2O.   Enough for 6~7 sequencing reactions.

1.  For PCR reactions that consist of a single product and PCR products over 100bp.

2.  Up to 95 samples (we need well A1 for control).

3.  Please indicate on your order form the volumes of your PCR reactions.

4.  If you want us to sequence the samples after cleanup, please provide your sequencing primers at 1 or 10 μM concentration and indicate on your order form where each primer should be placed.  If you have to use more than 3 or 4 primers per plate, it is best to provide us with a separate plate with primers to avoid pipetting errors.  

5.  To submit your PCR reactions, you will need to use a 96 well half skirted or semi skirted PCR plate.

  • We prefer samples to be submitted in a specific type of plate:
  • Catalog#: 4ti-0770/c
  • Description: FrameStar 96, semi-skirted
  • To order, please contact Dennis Harte @ 510-882-0640 or www.BasicScientific.com
  • If you are on campus users, please stop by 310 Barker and we are happy to provide the plates for free of charge.

6.  Please fill out "PCR Clean-up order form", submit the form with your samples and e-mail the form to dnaseq@berkeley.edu.

 

By Tube:  $1/sample


1.   For PCR reactions that consist of a single product and PCR products over 100bp.

2.  Please indicate on your order from the volume of your PCR reactions.

3.  If you want us to sequence the samples after cleanup, please provide your sequencing primers at 1 or 10 μM concentration and indicate on your order form where each primer should be placed.

4.  Please fill out "PCR Clean-up by tube order form" and submit the form with your samples.