How to Submit Samples
-
Please follow these guidelines when preparing your samples:
- Each sample tube (please do not use .2mL tubes) should contain one primer and
one template at the
concentrations indicated on the Order Form
- Template Preparation:
Please be sure that you do not have SALTS, PROTEINS, DETERGENTS,
RNA, CHROMOSOMAL DNA, ETHANOL, PHENOL, or TE in your sample.
- PCR Products:
Quantitate your PCR products on an agarose gel.
- Primers:
Please be sure your primers have a melting temperature (Tm) between 52°C and 60°C.
-
Order forms can be filled out and printed from this web site by following the
Order Form link. Order forms can also be found at all of
the sample drop off locations listed below.
-
Accounting Information for those with UC Berkeley accounts
We need your chartstring (e.g. 1-56620-19900-22233-44-none-FN248) or
a 10 digit
"speedtype" reference to your chartstring. You can use the
Chart of Accounts Validation Web Service.
to check that your on-campus accounting information is current. If it is
not, contact your lab manager, your accounting department or your PI.
Call us if you have any questions. 510.642.6383
- For those without UC Berkeley accounts:
Labs from other UC campuses should use the accounting information
you would normally use for any on-campus charge.
Labs from LBNL are charged the on-campus rate plus 3% and should have a
current purchase order number or IUT number. Call us if you have any questions.
Outside labs should generate a purchase order and attach it to their
order form or just send a check with your samples made out to "UC Regents".
-
Sample drop off locations:
- 237
Stanley Hall (second floor cold room) 11:10am, at the back on the
left on the shelf below the bench.
- 740
Latimer Hall (seventh floor cold room) 11:15am, on the right as you walk in,
to the right of the sink.
- 136
Life Sciences Addition (first floor cold room) at 11:30am, the sample
racks are to the
left on the shelf below the bench.
- 317
Barker Hall (third floor cold room) 11:30am, on the
right as you walk in.
- By Mail: mail your samples along with your order
form overnight or second day air
(no ice) to 303 Barker Hall #3202, Berkeley, California 94720-3202.
If your samples arrive before 12 noon, results will be sent the next
business day. Samples need to be put in a stiff
crush resistant secondary container such as a 50 mL falcon tube to
prevent crushing during shipping.
-
Turn around time: If you drop off your samples on time, or we receive them in
the mail before 12 noon we
will send out your results via e-mail by the
following business day if not sooner. Results can sometimes be delayed by equipment
problems. If you do not receive your results by e-mail or a notification of a delay
in one
business day do not hesitate to e-mail or call. We do not always get a message if
e-mail does not get through.
- Results will be sent by e-mail:
Text files(4KB) and electropherogram (chromatogram)
files(~300KB)
will be sent by e-mail.
- Viewing your results:
There is free software available on the internet that will allow you to view your results.
PC users can go to these websites:
Sequence Scanner from Applied Biosystems:
https://products.appliedbiosystems.com Sequence Scanner works on XP and Windows 2000.
or
Finch TV: http://www.geospiza.com/finchtv/ FinchTV works on OS X and
Windows.
or
Chromas
http://www.technelysium.com.au/chromas14x.html
MAC users can go to these websites:
4Peaks: http://www.mekentosj.com/4peaks/ 4Peaks works on OS X.
or
Finch TV: http://www.geospiza.com/finchtv/ FinchTV works on OS X and Windows.
or
Edit View works on OS 9.
MAC users may also need to use conversion software (before opening files with Edit View) available for free by
clicking on this
web link. Instructions for how to use the conversion software can be downloaded from
the same web link as the software.
If that web link does not work follow these directions:
Go to
www.appliedbiosystems.com.
Click on "Services & Support".
Click on "Software Dowloads".
Under "Select Product Software", use the drop down menu to choose "ABI PRISM 3100 Genetic
Analyzer"
Under "Select Software Type", use the drop down menu to choose "Conversion Utility"
Download "3100 Conversion Utility".
And "3100 Conversion Program Notes"
Let us know right away if you have any trouble viewing your files.
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