Genotyping

Genotyping:

  1. Before starting your project, please ensure that your dye sets are compatible with our Applied Biosystems 3730XL DNA Analyzers.
  2. The dye set best suited for our 3730XL is DS-33, which includes these dyes: 6-FAM, VIC, NED, PET, and LIZ (LIZ Size Standard). Please call us if you must use other dye sets.
  3. To submit your samples, you will need to use a 96 well half skirted or semi skirted PCR plate.  

    We prefer samples to be submitted in a specific type of plate:

    • Catalog#: 4ti-0770/c
    • Description: FrameStar 96, semi-skirted
    • To order, please contact Dennis Harte @ 510-882-0640 or www.BasicScientific.com
    • If you are on campus, please stop by 310 Barker and we are happy to provide the plates for free of charge.
  4. Please provide a minimum of 10 μl of PCR products in HiDi Formamide. Each well should have size standard.
  5. Applied Biosystems has Peak ScannerTM software v1.0 freely available to download  https://products.appliedbiosystems.com/ab/en/US/adirect/ab?cmd=catNavigate2&catID=603624
  6. Please fill out "Genotyping Order Form" and submit the form with your samples. Also please e-mail the form to dnaseq@berkeley.edu.
  7. Sample names cannot have spaces and only include the following punctuation:  -+.(){}_