Plasmid and Genomic DNA Prep

Plasmid Prep: 96well block format only.  $80/block for UC labs

This service utilizes magnetic bead technology for high-throughput purification of plasmid DNA from E. coli. It works for both high and low copy plasmids.

Contact us the day before submitting your cultures to confirm that someone will be available to process your samples. We cannot accept culture blocks on the weekend.

To take advantage of this, you will need:

  1. 96-well 2.2 ml deep well culture block with gas permeable seal (pick these up in 310 Barker)
  2. Up to 95 samples (please reserve well A1 for our sequencing control).
  3. Cultures grown in 1mL of 2XYT or Terrific broth with antibiotic in deep well culture block.
  4. Start from either a single colony or a glycerol stock, and culture with shaking at 300 rpm, 37 oC to mid or late log phase.
  5. Please bring your culture block with "the Plasmid Prep Order Form" to 310 Barker Hall.
  6. Once the plasmid prep is complete, we can perform the sequencing reaction for you.  Please indicate clearly which primer(s) you need us to use, and provide sufficient amount of 10μM concentration primer (unless it is one of our stock primers).
  7. Please email a copy of "the Full Plate Sequencing Form" to dnaseq@berkeley.edu

 

Genomic DNA Prep: 96well block format only.  $80/block for UC labs

Please contact Hitomi and/or Eileen directly to inquire.