PCR Clean Up

PCR Clean Up

We use magnetic SPRI bead technology to purify PCR products >100 bp, and elute in 40μl H2O (enough for 6-7 sequencing reactions) 

Submit PCR products in 10μl total volume according to these guidelines:

1000bp     80~160ng

600bp       50~100ng

400bp       30~60ng

200bp       15~30ng

Please ensure that all of your samples are normalized to the same volume. If you need more than 10 μl cleaned up, indicate this on your order form. 

 

By Plate:  $66/plate

1.  For PCR reactions that consist of a single product and PCR products over 100bp.

2.  Up to 95 samples (we need well A1 for sequencing control).

3.  Please indicate on your order form the volumes of your PCR reactions.

4.  If you want to sequence your clean PCR products, please provide primers at 10 μM concentration and indicate on your order form where each primer should be placed. If you have more than 3 or 4 primers per plate, it is best to provide us with a corresponding primer plate (aliquot 5ul of 1μM primer per well).  

5.  To submit your PCR reactions, you will need to use a 96 well half skirted or semi skirted PCR plate.

  • We prefer samples to be submitted in a specific type of plate:
  • Catalog#: 4ti-0770/c
  • Description: FrameStar 96, semi-skirted
  • To order, please contact Dennis Harte @ 510-882-0640 or www.BasicScientific.com
  • Come to 310 Barker - we'll give you plates free of charge.

6.  Please fill out "PCR Clean-up order form", submit the form with your samples and e-mail the form to dnaseq@berkeley.edu.

 

By Tube:  $1.50/sample


1.  For PCR reactions that consist of a single product over 100bp.

2.  Please indicate on your order from the volume of your PCR reactions.

3.  If you want us to sequence the samples after cleanup, please provide your sequencing primers at 1 or 10 μM concentration and indicate on your order form where each primer should be placed.

4.  Please fill out "PCR Clean-up by tube order form" and submit the form with your samples.