University of California, Berkeley | Department of Molecular & Cell Biology
Fragment Analysis
Fragment Analysis:
- Before you start your project, please make sure that you use the dye sets compatible with our Applied Biosystems 3730XL DNA Analyzers.
- The dye set best suited for our 3730XL is DS-33, which includes these dyes: 6-FAM, VIC, NED, PET, and LIZ (LIZ Size Standard). Please call us if you must use other dye sets.
- To submit your samples, you will need to use a 96 well half skirted or semi skirted PCR plate.
We prefer samples to be submitted in a specific type of plate:
- Catalog#: 4ti-0770/c
- Description: FrameStar 96, semi-skirted
- To order, please contact Dennis Harte @ 510-882-0640 or www.BasicScientific.com
- If you are on campus, please stop by 310 Barker and we are happy to provide the plates for free of charge.
- Please provide a minimum of 10 μl of PCR products in HiDi Formamide. Each well should have size standard.
- The Applied Biosystems web site has free software available to download. Click on the link below to down load Peak ScannerTM software v1.0. https://products.appliedbiosystems.com/ab/en/US/adirect/ab?cmd=catNavigate2&catID=603624
- Please fill out "the genotyping order form" and submit the form with your samples. Also please e-mail the form to dnaseq@berkeley.edu.
- Sample names may not have spaces and only include the following punctuation: -+.(){}_