Information

Facility Location: 310 Barker Hall

Sample drop off locations and sample pick up time:

  • 237 Stanley Hall (second floor cold room) 11:10am, on the rear left on the shelf below the bench.
  • 740 Latimer Hall (seventh floor cold room) 11:15am, on the right as you walk in, to the right of the sink.
  • 136 Life Sciences Addition (first floor cold room) at 11:30am, the sample racks are rear left on the shelf below the bench.
  • 183 Li Ka Shing (first floor freezer farm) at 11:30am, the sample racks are on top of a small cabinet on the north corner
  • 317 Barker Hall (third floor cold room) 11:30am, on the right as you walk in.
  • 329 Energy Biosciences Building (third floor cold room) 11:30am, on the rear left on the shelf below the bench.
  • By Mail: mail your samples along with your order form overnight or second day air (no ice) to:

 

             UC Berkeley DNA Sequencing Facility
             310 Barker Hall #3202,
             Berkeley, California 94720-3202.
             Samples need to be put in a secondary container such as a 50 mL falcon tube to prevent from crushing during shipping.

WE ACCEPT SAMPLES ALL DAY.  PLEASE COME TO 310 BARKER HALL WHEN YOU MISS SCHEDULED PICK UP TIME.

 

Turnaround Time:

  • You should receive your results via e-mail by the following business day if not sooner.  
  • Early bird catches the worm:  The results of the first three plates of the day are e-mailed by 7PM on the same day.
  • Please let us know right away if you do not receive your results by the expected date.

 

Accounting Information:

  • For those with UC Berkeley accounts: We need your chartstring (e.g. 1-56620-19900-22233-44-none-FN248) or a 10 digit "speed-type" reference to your chartstring. You can use the Chart of Accounts Validation Web Service to check that your on-campus accounting information is current. If it is not, contact your lab manager, your accounting department or your PI.
  • For those without UC Berkeley accounts:  Labs from other UC campuses should use the accounting information you would normally use for any on-campus charge.  Labs from CHORI and LBNL are charged the on-campus rate plus the cost of billing (7.49%) and should have a current purchase order number or IUT number.  Outside labs should generate a purchase order and attach it to their order form or send a check with your samples made out to "UC Regents".
  •  Call us if you have any questions. (510) 642-9601

 

How do view my chromatogram files:

  1. Each sample generates two files. The file ending .seq is a text file and the file ending .ab1 is a chromatogram. We strongly recommend that you visually inspect the chromatogram files.
  2. There is free software available on the Internet that will allow you to view your results.
  3. PC users can go to these websites:
  4. MAC users can go to these websites:

 FAQ

  • When will I receive my results?

 We will notify you via e-mail.  We should have results for you by the next business day. If you don't receive your results, please contact us right away.

  • How do I get my results?

Results will be sent by e-mail.  You will receive text files (.seq files, 4KB) and electropherogram files (.abi files, ~300KB) for each sample.

  • How long does it take to process sequencing samples?

       It takes roughly 6 hours:

  1. PCR reaction assembly and data entry  30min
  2. PCR  2hours and 20min
  3. Post PCR clean-up  40min 
  4. Sequencing run  2hours
  5. Date analysis/e-mailing data  30min