Information

We accept samples in 310 Barker Hall all day from 9am to 8pm, Call 510-642-6383 after 5pm

We collect samples at 11:30 am 6:30 pm at the following locations:

 

  • By Mail: send your samples and order form overnight or second day air (no ice) to: 

             UC Berkeley DNA Sequencing Facility
             310 Barker Hall #3202
             Berkeley, California 94720-3202


             Place samples in a secondary container such as a 50 mL falcon tube to prevent crushing during shipping.

  

Turnaround Time:

  • You should receive your results via e-mail by the following business day if not sooner.  
  • Early bird catches the worm:  The results of the first three plates of the day are e-mailed by 7PM on the same day.
  • Please let us know right away if you do not receive your results by the expected date.

 

Accounting Information:

  • For UC Berkeley accounts: We need your chartstring (e.g. 1-56620-19900-22233-44-none-FN248) or a 10 digit "speed-type" reference to your chartstring. You can use the Chart of Accounts Validation Web Service to check that your on-campus accounting information is current. If it is not, contact your lab manager, your accounting department or your PI.
  • For other UC-system accounts: Use the accounting information you would normally use for any on-campus charge. Labs from CHORI and LBNL are charged the on-campus rate plus the cost of billing (10%) and should have a current purchase order number or IUT number.  Outside labs are charged 58% surcharge and should generate a purchase order and attach it to their order form or send a check with your samples made out to "UC Regents".
  • Call us if you have any questions  (510) 642-9601

 

How to view my chromatogram files:

Each sample generates two files, a text file (.seq) and a chromatogram (.ab1). We strongly recommend visual inspection of your chromatograms. Free software is available for download

Mac users

PC users

 

 

Sequence Ananlysis and Design Software

List of sequence alignment software

FAQ

  • When will I receive my results?

 We will notify you via e-mail.  We should have results for you by the next business day. If you don't receive your results, please contact us right away.

  • How do I get my results?

Results will be sent by e-mail.  You will receive text files (.seq files, 4KB) and electropherogram files (.abi files, ~300KB) for each sample.

  • How long does it take to process sequencing samples?

       It takes roughly 6 hours:

  1. PCR reaction assembly and data entry  30min
  2. PCR  2hours and 20min
  3. Post PCR clean-up  40min 
  4. Sequencing run  2hours
  5. Data analysis/e-mailing data  30min